LOCUS       sequence-430549-       13012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA 
            targeting B. theta amylopectin SusC gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 13012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH504
FEATURES             Location/Qualifiers
     source          1..13012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    201..217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     ncRNA           230..266
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        287..362
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           369..436
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             860..1942
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(879..898)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(2283..2309,2310..2312,2313..4799,4800..4802,
                     4803..6389)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(11428..11496,11497..12288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11646..11665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            12419..12528
                     /note="incP origin of transfer"
     rep_origin      join(12699..13012,1..75)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
       61 acttaacggc tgacatggga attcccctcc accgcggtgt attaatgcgg ctgcccctgt
      121 acaaagaaaa ttcgacaaac tgttattttt ctatctattt atttgaattg tgagcggata
      181 acaattacct ttgtcggcaa ttgtgagcgg ataacaatta tgatgttgtc tgatgagtcc
      241 gtgaggacga aacgagtaag ctcgtcacaa cattggcacc gataacgttt tagagctaga
      301 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      361 gcttttttgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg
      421 gcatggcgaa tgggacgcac tctaatcgtt atcggagtgc ttttagatta ctaatcaaat
      481 tgcttctact aattgcctat cttccagtga tggaacagca tttgaacgca ttggctgcaa
      541 caatcagcct tgatctggaa gaagcaatga aagctgctgt taagtctccg aatcaggtat
      601 tgttcctgac aggtgtattc ccatccggta aacgcggata ctttgcagtt gatctgactc
      661 aggaataaat tataaattaa ggtaagaaga ttgtaggata agctaatgaa atagaaaaag
      721 gatgccgtca cacaacttgt cggcattctt ttttgtttta ttagttgaaa atatagtgaa
      781 aaagttgcct aaatatgtat gttaacaaat tatttgtcgt aactttgcac tccaaatctg
      841 tttttaacat atggcactag tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
      901 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
      961 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     1021 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     1081 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     1141 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaaacggcgg tgcacaatct
     1201 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     1261 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     1321 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     1381 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     1441 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     1501 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     1561 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     1621 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     1681 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     1741 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     1801 gggcaatcag ctgttgcccg tatcactggt gaaaagaaaa accaccctgg cgcccaatac
     1861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     1921 ccgactggaa agcgggcagt gagctttcct cggtaccaaa ttccagaaaa gaggcctccc
     1981 gaaagggggg ccttttttcg ttttggtcct acttgtgcct gttctatttc ctatgcggtt
     2041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2101 ggataaccgt attaccgcct ttgagtgagc tgatacatcc caaatgctgc tcggtaccaa
     2161 agacgaacaa taagacgctg aaaagcgtct tttttcgttt tggtccgata aagtttggaa
     2221 gataaagcta aaagttctta tctttgcagt ccgaaataaa gacatataaa agaaaagaca
     2281 ccatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc ggatgggcgg
     2341 tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc
     2401 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg
     2461 aagcgactcg tcttaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     2521 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     2581 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     2641 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     2701 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     2761 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     2821 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     2881 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     2941 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     3001 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     3061 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     3121 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     3181 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt
     3241 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     3301 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     3361 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     3421 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc
     3481 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     3541 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     3601 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     3661 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     3721 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata
     3781 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     3841 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc
     3901 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     3961 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     4021 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     4081 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     4141 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     4201 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     4261 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     4321 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg
     4381 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     4441 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     4501 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     4561 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc
     4621 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     4681 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     4741 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatg
     4801 ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt
     4861 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga
     4921 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     4981 taacgaaagc tgaacgtggt ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     5041 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga
     5101 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     5161 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     5221 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga
     5281 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     5341 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact
     5401 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac
     5461 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     5521 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     5581 tacagacagg cgaattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta
     5641 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag
     5701 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg
     5761 ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa aatccgattg
     5821 actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta
     5881 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat
     5941 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta
     6001 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg
     6061 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg
     6121 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca
     6181 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag
     6241 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa
     6301 aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca
     6361 ttgatttgag tcagctagga ggtgactgat tctttgtacc cgataattgc ctatcttcca
     6421 gtgatggaac agcatttgtg cattggctgc aacaatcagc cttacttgtg cctgttctat
     6481 ttccgaaccg accgcttgta tgaatgcatc aaaattcgtt ttctctacgt tggattcctt
     6541 gttgctcata ttgtgatgat aatttctaca aatatagtca ttggtaacta tctatgaaac
     6601 tgtttgatac ttttatcagt ccagtagttt tacaaggtct ttcttcattt cttcgtcaat
     6661 atccctgtat cgtctgaaag ctttgcttcc ctccttgtgt cccgacagtg cggaaacaag
     6721 gttcgggtct ttcacttttt tatagatatt gccgataaac gtacgtcttg ccagatggct
     6781 gcttgccact tcataaatag gtcgtttgat ttcgttgtgc gtcaacgggt ctaagattgt
     6841 tacgatgcgg tcaactccag ctaatttgaa tatctttttt atggcatcat tgtacttttg
     6901 ctcggatatg aacggcaaca gttttccctc atattctttg tagcgttcaa ggatttcttt
     6961 cgctttgtcg ttaagtggaa cacgtaccgt aaccggattc ccctctttgg ttttcttggg
     7021 aatatattct atggcttcat tgaccacatt tagtttggtc attcggtaca ggtcgctcac
     7081 cctgcatcct atcagtgtct gaaatatgaa tatatccctc tgtattgcca gttgtggggt
     7141 ggcagaaagg tctgcattaa aaatcctgtc cctttcttcg agtgttatat aataaggtgt
     7201 accatatgta cactcctcta tcggaaactt gtcgaaaggt ctgtttgtgg tgcgtttgtt
     7261 atcgaagcac cacaggaaga atgtgcgtat tcttgaaaaa cagtctatca gcgtgttttt
     7321 gcttctgggc tgtggtgtcc tcttttcggg aatggcttca taaatgctcg ggtaaagttc
     7381 ataatactgg tattcgttct gaaagaaatc ccacatatcc cgaagcgtgt caggtgttac
     7441 caaatccaca tcaaggataa agcccttttg tcctctcttt gtagccctta catatagttc
     7501 ataacgcagt aaggctcttt tgacaacccg gaaatttttc tttcgtactt ccgacaaagg
     7561 gtgcttgttt agaaattcat cgaatagttc tccaatggta ggcttgataa ccacttcctc
     7621 cggcaaaaaa tatttttcag gatggtaaaa tttatcaagt gttgttttta gccattcttt
     7681 gtctattgct tccttttctt gttgatatac cttctcgata taggttttca actggcgtat
     7741 ctcttcattt atatgggtac gcatttcttc attgcagaca gctttcgttt ttacacattc
     7801 gtctttatca tcccatagat tgggattgat ggctaactga gtaggagcaa cggagtctaa
     7861 ctgccttccg tttctaaaac ggacatagat tgtagccatg gattctgtat catatcgttt
     7921 ggctgctttt ttaatgataa aggttacttt catagacttt caggttgaat tttactctgc
     7981 tgcaaatata aatattttcc ccagcatttt ccccacatct gctaaatatt ttgcaattcg
     8041 attaaacttg gattaaaatt taataggatt ataacatatt gaaatacagt gtaattgtgg
     8101 cgtttttccg catttttctt tttacccaca ttttccccac tttaattatt tcaaatgcgg
     8161 ggtctgggta caagaaagaa agctaagtat ttgatagttc aatacttagc tttttctttt
     8221 gcttgaattt tccccacatt ttccccacac gtgcaaaaaa tatagcagta agtcattatt
     8281 tcttttggtt gaacgtagag agtagcgata ttaaaagaat ccgatgagaa aagactaata
     8341 tttatctatc cattcagttt gatttctcag gactttacat cgtcctgaaa gtatttgttg
     8401 gtaccgagga cgcgtaaaca tttacagttg catgtggcct attgtttagg acgcgttatc
     8461 tccttaacgt acgttttcgt tccattggcc ctcaaacccc gttatataca ttcatgtcca
     8521 tttatgtaaa aaatcctgct gaccttgttt atgtcttgtc agtcaccatt tgcaaaacca
     8581 tatttgaccc tcaaagaggc tgaatttgat aagcaacttg ctacatactc ataataagga
     8641 gctaaataga acacgaatgg gaaatactca aatgccaaac taaagaagat attggccaaa
     8701 ataaacgcta taccgagaga gaaacttgat ttttcaactt cctaaaacag tgttgttcaa
     8761 acatttctac ttatttgtac ttaccagttg aacctacgtt tccctaataa aatgtctatg
     8821 gtaaaaagtt aaaaaatcct cctacttttg ttagatatat ttttttgtgt aattttgtaa
     8881 tcgttatgcg gcagtaataa tatacatatt aatacgagtt aggaatcctg tagttctcat
     8941 atgctacgag gaggtattaa aaggtgcgtt tcgacaatgc atctattgta gtatattatt
     9001 gcttaatcca aatgaatatt ataaatttag gaattcttgc tcacattgat gcaggaaaaa
     9061 cttccgtaac cgagaatctg ctgtttgcca gtggagcaac ggaaaagtgc ggctgtgtgg
     9121 ataatggtga caccataacg gactctatgg atatagagaa acgtagagga attactgttc
     9181 gggcttctac gacatctatt atctggaatg gtgtgaaatg caatatcatt gacactccgg
     9241 gacacatgga ttttattgcg gaagtggagc ggacattcaa aatgcttgat ggagcagtcc
     9301 tcatcttatc cgcaaaggaa ggcatacaag cgcagacaaa gttgctgttc aatactttac
     9361 agaagctgca aatcccgaca attatattta tcaataagat tgaccgagcc ggtgtgaatt
     9421 tggagcgttt gtatctggat ataaaagcaa atctgtctca agatgtcctg tttatgcaaa
     9481 atgttgtcga tggatcggtt tatccggttt gctcccaaac atatataaag gaagaataca
     9541 aagaatttgt atgcaaccat gacgacaata tattagaacg atatttggcg gatagcgaaa
     9601 tttcaccggc tgattattgg aatacgataa tcgctcttgt ggcaaaagcc aaagtctatc
     9661 cggtgctaca tggatcagca atgttcaata tcggtatcaa tgagttgttg gacgccatca
     9721 cttcttttat acttcctccg gcatcggtct caaacagact ttcatcttat ctttataaga
     9781 tagagcatga ccccaaagga cataaaagaa gttttctaaa aataattgac ggaagtctga
     9841 gacttcgtga cgttgtaaga atcaacgatt cggaaaaatt catcaagatt aaaaatctaa
     9901 aaactatcaa tcagggcaga gagataaatg ttgatgaagt gggcgccaat gatatcgcga
     9961 ttgtagagga tatggatgat tttcgaatcg gaaattattt aggtgctgaa ccttgtttga
    10021 ttcaaggatt atcgcatcag catcccgctc tcaaatcctc cgtccggcca gacaggcccg
    10081 aagagagaag caaggtgata tccgctctga atacattgtg gattgaagac ccgtctttgt
    10141 ccttttccat aaactcatat agtgatgaat tggaaatctc gttatatggt ttaacccaaa
    10201 aggaaatcat acagacattg ctggaagaac gattttccgt aaaggtccat tttgatgaga
    10261 tcaagactat atacaaagaa cgacctgtaa aaaaggtcaa taagattatt cagatcgaag
    10321 tgccgcccaa cccttattgg gccacaatag ggctgactct tgaaccctta ccgttaggga
    10381 cagggttgca aatcgaaagt gacatctcct atggttatct gaaccattct tttcaaaatg
    10441 ccgtttttga agggattcgt atgtcttgcc aatccgggtt acatggatgg gaagtgactg
    10501 atctgaaagt aacttttact caagccgagt attatagccc ggtaagtaca cctgctgatt
    10561 tcagacagct gaccccttat gtcttcaggc tggccttgca acagtcaggt gtggacattc
    10621 tcgaaccgat gctctatttt gagttgcaga taccccaagc ggcaagttcc aaagctatta
    10681 cagatttgca aaaaatgatg tctgagattg aagacatcag ttgcaataat gagtggtgtc
    10741 atattaaagg gaaagttcca ttaaatacaa gtaaagacta tgcatcagaa gtaagttcat
    10801 acactaaggg cttaggcatt tttatggtta agccatgcgg gtatcaaata acaaaaggcg
    10861 gttattctga taatatccgc atgaacgaaa aagataaact tttattcatg ttccaaaaat
    10921 caatgtcatc aaaataatgg tataacctct ccttaattta ttgcatctct tttcgaatat
    10981 ttatgttttt tgagaaaaga acgtactcat ggttcatccc gatatgcgta tcggtctgta
    11041 tatcagcaac tttctatgtg tttcaactac aatagtcatc tattctcatc tttctgagtc
    11101 caccccctgc aaagcccctc tttacgacat aaaaattcgg tcggaaaagg tatgcaaaag
    11161 atgtttctct ctttaagaga aactcttcgg gatgcaaaaa tatgaaaata actccaattc
    11221 accaaattat atagcgactt ttttacaaaa tgctaaaatt tgttgatttc cgtcaagcaa
    11281 ttgttgagca aaaatgtctt ttacgataaa atgatacctc aatatcaact gtttagcaaa
    11341 acgatatttc tcttaaagag agaaacacct ttttgttcac caatccccga cttttaatcc
    11401 cgcggccatg attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    11461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    11521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    11581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    11641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    11701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    11761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    11821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    11881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    11941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    12001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    12061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    12121 aaatctggag ccggtgagcg tgggtcacgc ggtatcattg cagcactggg gccagatggt
    12181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    12241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    12301 gtttactcat aacgcgtcaa ttcgaggggg atcaattccg tgataggtgg gctgcccttc
    12361 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
    12421 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
    12481 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
    12541 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
    12601 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
    12661 aacggaattg atccggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
    12721 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
    12781 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
    12841 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
    12901 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
    12961 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gg
//
