LOCUS       sequence-43055-e        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.1 (FF570)
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(588..840)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(860..1411)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1453..1632)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1869..1891
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1883..1900
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1884..1900
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1910..1929
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1910..1928
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1953..1969
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1954..1970
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2000..2018
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2000..2017
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(2051..2758)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4158..4503
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4446..4466
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4550..4674
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4699..4704,4705..4722,4723..4729)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4783..5442
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4849..4868)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5784..6089
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5968..5987
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6130..6254)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6261..6902
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(6924..6942)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6963..6979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6963..6979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6976..6998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6987..7003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7011..7017,7018..7036,7037..7042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7057..7078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7195..7212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7317..7341
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7432..8020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7512..7531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8191..8979)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(9270..9298,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
      601 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
      661 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
      721 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
      781 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
      841 tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg ggcggcaccg
      901 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
      961 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
     1021 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
     1081 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
     1141 cggtggactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcga
     1201 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
     1261 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
     1321 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
     1381 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga gttgagagtg
     1441 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     1501 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     1561 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca
     1621 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     1681 cataacgtga ctcccttaat tctccgctca tgtatcgata acattaacgt ttacaatttc
     1741 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1801 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1861 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     1921 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
     1981 tatcgaattc ctgcagccca tttaggtgac actatagaat atcactagta agctagcttg
     2041 catgccggtc ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac
     2101 gatttgtcgt cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa
     2161 aaatgctgca taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt
     2221 tgaatggtgc ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc
     2281 acccatgcgc gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct
     2341 cggtgtgtcg tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa
     2401 tcagtctttt aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa
     2461 aacgcaattg tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca
     2521 caatatattg ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt
     2581 gaacggagaa tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga
     2641 accgaaaccg gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa
     2701 cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcaggg
     2761 atcccgcatc agatctatca aacacataga tttacgaaga tctctaacat taaatcatca
     2821 gagagataag tttagggagc aagctctttc gcttacacag agaatcacag aggaaagaga
     2881 ctttaagaaa actacaactt ttttgaccac tgtgatttct ctctgcaagc gaaagtactt
     2941 aatcaccaac acagtagatt gatatatttg ggtttatttg tgagaagaag aattcactag
     3001 tggatcccgc actcgagtgt taatcagaaa aactcagatt aatctacaaa ttcgatcgca
     3061 caaactagaa actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga
     3121 caaaactcaa attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa
     3181 gatgatatct aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa
     3241 accaaagaac atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa
     3301 taaggaacac agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga
     3361 ttttgagaaa ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag
     3421 aagaagaaga agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa
     3481 tagctaataa taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc
     3541 taattgcctt atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact
     3601 cgtgtgtgcc ggctgcaacc actttgacgc cgtttattac tgactcgtcg gggggatcca
     3661 ttcggtcccc agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga
     3721 gaggcttacg cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc
     3781 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     3841 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     3901 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc
     3961 actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
     4021 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
     4081 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
     4141 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     4201 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     4261 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     4321 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     4381 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     4441 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga
     4501 acacggggga cgagctgtga tttttctcta caagcgaatc tagaggatca caagtttgta
     4561 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     4621 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcat attggcggcc
     4681 ccattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     4741 agttaggagc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     4801 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     4861 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     4921 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     4981 atgaatgctc atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     5041 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     5101 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     5161 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     5221 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     5281 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     5341 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     5401 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg agccggctta
     5461 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     5521 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     5581 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     5641 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     5701 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     5761 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     5821 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggc cgacggatgg
     5881 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     5941 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     6001 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     6061 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     6121 cacctcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     6181 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     6241 ttgtacaaag tggtgatcct agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa
     6301 gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg
     6361 cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta
     6421 ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata
     6481 tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt
     6541 tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc
     6601 gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg
     6661 cttattcact aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat
     6721 acaagtatgt cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga
     6781 atccttgtca gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt
     6841 gagtatgaaa atatttttta atgcatttta tgacttgcca attgattgac aacatgcatc
     6901 aatcggagct ccagcttttg ttccctttag tgagggttaa ttccgagctt ggcgtaatca
     6961 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7021 gccggaaghc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatga aggccttgac
     7321 aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc
     7381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7861 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag
     7921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8161 aagtatatat gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc
     8221 tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg
     8281 tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     8341 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     8401 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     8461 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     8521 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     8581 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     8641 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     8701 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     8761 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     8821 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     8881 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     8941 tagttgagtc gatacttcgg cgatcaccgc ttccctcata acaccccttg tattactgtt
     9001 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     9061 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
     9121 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
     9181 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
     9241 gctggatgat ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggct
//
