LOCUS       sequence-430572-       11623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative response-polarity hybrid voltage indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11623)
  TITLE     Peng Zou Lab Plasmids 2024
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FCK-CaMKII-Solaris-WPRE
FEATURES             Location/Qualifiers
     source          1..11623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3900..3909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6387..6975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6440..6460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6858..6869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6978..6994)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6979..6995)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7500..7680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7706..7723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7712..7936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7982..8410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8069..8088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8279..8300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8419..8439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8463..8673
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8485..8681
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8532..8667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8594..8613
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8729..8776
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8795..9169
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9299..9420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9336..9355)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9390..9409
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9469..9485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9469..9485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9482..9504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9493..9509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9517..9523,9524..9541,9542..9547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9562..9583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9700..9717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9871..10459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9951..9970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10630..11421,11422..11490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11253..11272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11491..11595)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
     3901 ccaccatggc tgacgtggaa accgagaccg gcatgattgc acagtggatt gtctttgcta
     3961 ttatggctgc tgctgctatt gcttttggag tggctgtgca ctttcggcct tcagagctga
     4021 agagcgcata ctatatcaac attgccatct gcactatcgc cgctaccgct tactatgcaa
     4081 tggccgtgaa ctaccaggac ctgacaatgt ttgcccgcga gaccttccag gccttccgca
     4141 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
     4201 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
     4261 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
     4321 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
     4381 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
     4441 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
     4501 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg
     4561 agatttccgg cgagccaacc actggaggca gcggaggcac aggaggcagc ggaggcacag
     4621 gaggcagcat ggcagaaatc ggtactggct ttccattcga cccccattat gtggaagtcc
     4681 tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc
     4741 tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat gttgcaccga
     4801 cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa ccagacctgg
     4861 gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc ctgggtctgg
     4921 aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac tgggccaagc
     4981 gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct atcccgacct
     5041 gggacgaatg gccagaattt gccgggaatg gtgaaaggca ggtggtctac gcaagatata
     5101 tttcctgggt gctgaccaca ccactgctcc tgctcgatct catcgtcatg accaagatgg
     5161 gcggagtgat gatttcttgg gtcatcggcg cagacatttt catgatcgtg tttggtattc
     5221 tgggcgcctt cgaggatgaa cacaagttca aatgggtgta ctttatcgct ggatgtgtga
     5281 tgcaggcagt cctgacatac gggatgtata acgccacttg gaaagacgat ctgaagaaaa
     5341 gccccgagta ccatagctcc tatgtcagtc tgctcgtctt cctgtcaatc ctctgggtgt
     5401 tttatcctgt cgtgtgggct ttcgggtctg gtagtggcgt gctgtccgtc gacaatgagg
     5461 ccattctcat gggaatcctg gatgtgctcg ctaagccact gtttggaatg gggtgcctca
     5521 ttgcccatga gactatcttc aagaccggtg ccgccgaccg cccggtagta gcagtgagca
     5581 aggcggccgc caagagcagg atcaccagcg agggcgagta catccccctg gaccagatcg
     5641 acatcaacgt gaacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     5701 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5761 gctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5821 tcctgtcccc tcatttcacc tttggctcca aggcctacgt gaagcacccc gccgacatcc
     5881 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
     5941 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     6001 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga
     6061 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggtgcc ctgaagggca
     6121 agatcaagat gaggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca
     6181 cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg
     6241 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     6301 gccactccac cggcggcatg gacgagctgt acaagttctg ctacgagaac gaggtgtaat
     6361 gagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6421 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6481 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6541 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6601 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6661 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6721 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6781 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6841 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6901 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6961 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7021 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7081 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7141 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7201 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7261 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7321 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7381 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7441 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7681 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7741 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7801 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7861 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7921 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     7981 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8401 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8521 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8581 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     8641 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     8701 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     8761 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     8821 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     8881 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     8941 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9001 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9061 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9121 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9181 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9241 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9301 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9361 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9421 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9481 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9541 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9601 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9661 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9781 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9901 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9961 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10021 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10081 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10141 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10201 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10261 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10321 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10381 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10441 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10501 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10561 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10621 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10681 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10741 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10801 agcaataaac cagccagccg gaagggccga tcgcagaagt ggtcctgcaa ctttatccgc
    10861 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10921 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10981 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11041 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11101 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11161 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11221 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11281 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11341 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11401 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11461 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11521 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11581 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
