LOCUS       sequence-430574-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mus musculus histone 1 in Gateway entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Kim RK, Truby NL, Silva GM, Picone JA, Miller CS, Baldwin AN, Neve 
            RL, Cui X, Hamilton PJ
  TITLE     Histone H1x in mouse ventral hippocampus associates with, but does 
            not cause behavioral adaptations to stress.
  JOURNAL   Transl Psychiatry. 2024 Jun 5;14(1):239. doi: 
            10.1038/s41398-024-02931-x.
  PUBMED    38834575
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl 
            Psychiatry."; date: "2024-06-5"; volume: "14(1):239. doi"; pages: " 
            10.1038/s41398-024-02931-x"
COMMENT     Sequence Label: H1x
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1272..1371)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1388..1407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1389..1407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1412..1428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1412..1428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1530..1549)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1541..2350
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1608..1627)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2497..3085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2986..3005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat gagcgtggaa ctggaggaag ctctccctcc
      721 caccagcgct gacggaaccg ctaggaagac cgccaaggct ggaggcagcg ctgctcccac
      781 ccaacccaag aggagaaaga ataggaagaa aaaccagccc ggcaagtaca gccagctggt
      841 ggtggagact attaggaagc tgggcgaaag aggcggatcc agcctcgcta ggatctacgc
      901 cgaggccaga aaggtggctt ggttcgatca gcagaacgga aggacttacc tcaagtacag
      961 catcagagct ctggtccaga acgacactct gctgcaagtg aagggcaccg gcgccaacgg
     1021 cagcttcaaa ctcaatagga agaagctcga gggcggcgct gagagaagag gagctagcgc
     1081 tgctagctcc cccgccccta aggctaggac tgctgctgcc gataggaccc ccgccagacc
     1141 tcagcccgag agaagggccc acaagagcaa aaaggccgcc gctgctgcta gcgccaagaa
     1201 ggtgaagaag gctgccaagc ctagcgtgcc caaagtgcct aagggaagga agtgaaaggg
     1261 tgggcgcgcc gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1321 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1381 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1441 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1501 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1561 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1621 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1681 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1741 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1801 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1861 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1921 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1981 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2041 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2101 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2161 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2221 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2281 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2341 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2401 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2461 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2521 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2581 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2641 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2701 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2761 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2821 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2881 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2941 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3001 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3061 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3121 cttttgctgg ccttttgctc acatgtt
//
