LOCUS       sequence-430580-        1706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mini-mutagenesis plasmid for kappa light chain YSD Fabs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Petersen BM, Kirby MB, Chrispens KM, Irvin OM, Strawn IK, Haas CM, 
            Walker AM, Baumer ZT, Ulmer SA, Ayala E, Rhodes ER, Guthmiller JJ, 
            Steiner PJ, Whitehead TA
  TITLE     An integrated technology for quantitative wide mutational scanning 
            of human antibody Fab libraries.
  JOURNAL   Nat Commun. 2024 May 10;15(1):3974. doi: 10.1038/s41467-024-48072-z.
  PUBMED    38730230
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-10"; volume: "15(1):3974. doi"; pages: " 
            10.1038/s41467-024-48072-z"
COMMENT     Sequence Label: pMMP_kappa
FEATURES             Location/Qualifiers
     source          1..1706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      152..740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     641..660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1019..1678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1593..1612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1679..1706,1..75))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
       61 cttacgtgcc gatcatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg
      841 cggccgctac cagacttcaa ctgttcatca gatggtggaa aaatgaagac ggatggagca
      901 gctacagaga gaccagctac atataaggtc tcacaagggc ccgcaggtgt tccccctggc
      961 ccccagcagc aagagcacca gcggcggcac agcggccgcc ctcagcgcct taaaaaaatt
     1021 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1081 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1141 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1201 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1261 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1321 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1381 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1441 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa
     1501 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1561 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1621 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1681 tagcttcctt agctcctgaa aatctc
//
