LOCUS       sequence-430583-       10062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Fucci cell cycle indicator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Sato H, Wu B, Delahaye F, Singer RH, Greally JM
  TITLE     Retargeting of macroH2A following mitosis to cytogenetic-scale 
            heterochromatic domains.
  JOURNAL   J Cell Biol. 2019 Jun 3;218(6):1810-1823. doi: 
            10.1083/jcb.201811109. Epub 2019 May 20.
  PUBMED    31110057
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201811109"; journalName: "J Cell Biol"; date: 
            "2019-06-3- 3"; volume: "218"; issue: "6"; pages: "1810-1823"
COMMENT     Sequence Label: pCCL-CellCycle
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(946..1149)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(975..995)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1150..1529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1630..1650)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1630..1648)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1669..1685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1669..1685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1682..1704)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1693..1709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1717..1723,1724..1741,1742..1747))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1762..1783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1900..1917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2071..2659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2151..2170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2830..3621,3622..3690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3453..3472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3691..3795)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3821..4276)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3958..3979)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4170..4189
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4403..4425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4417..4434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4418..4434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4444..4463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4444..4462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4483..4618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4658..4779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4669..4688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4723..4742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4851..5084
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5171..5759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5277..5288
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5686..5706
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5791..6120)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(6151..6174)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6181..6888)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6456..6475)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6725..6743
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(6895..6948)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6955..6978)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6994..7266)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(7303..8001)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(8034..9245)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(8178..8197)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    9302..9419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9686..9730)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9915..10062,1..86)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
       61 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
      121 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
      181 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
      241 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      301 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      361 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      421 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      481 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      541 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      601 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      661 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      721 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      781 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      841 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      901 aggctcagat ctggtctaac cagagagacc ccggttcact aaacgagctc tgcttatata
      961 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     1021 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     1081 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     1141 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     1201 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     1261 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     1321 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     1381 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     1441 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     1501 ctattaataa ctagtcaata atcaatgtca acatggcggt aatgttggac atgagccaat
     1561 ataaatgtac atattatgat atggatacaa cgtatgcaat ggccaagctt gcagctccag
     1621 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     1681 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1741 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1801 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1861 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2701 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2761 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     3841 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3901 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3961 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4021 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     4081 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4141 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4201 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4261 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4321 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4381 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     4441 gcgtaatacg actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc
     4501 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4561 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4621 gcggagttag gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga
     4681 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4741 gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca
     4801 tttctttgca agttataaat actgaataat aagatgacat gaactactac tgctagagat
     4861 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     4921 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     4981 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     5041 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     5101 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtaccgagct
     5161 cgaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     5221 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     5281 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     5341 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     5401 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     5461 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     5521 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     5581 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     5641 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     5701 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     5761 tcgacgttat ctagatccgg tggatcctta cagcgccttt ctccgttttt ctgccacttc
     5821 cttccaatac tgagaggatg gattttcttt aatcataaga tcaaatgact cctgggtgac
     5881 tcctccaaga tttttatttt cactagattc tgggacaata accccagggc tggaagttgt
     5941 agatgttaag tggtcattcc gatgtttcct tttggacaag cctgcggaca gctcattttc
     6001 tcttccaaca agagatccag atgcagaagg ctgaatcatc ttcagagttc ttcttgggac
     6061 agaactattc tttatattct ctttgatttc ttcttgtttc tgcttcatac tgggattcat
     6121 ctcgagcggc cgccagtgtg atggatatcc cttgtcatcg tcatccttgt aatcgccgcc
     6181 cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc
     6241 cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc
     6301 gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg
     6361 gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg
     6421 gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
     6481 ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga
     6541 ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt
     6601 gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta
     6661 ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc
     6721 acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
     6781 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat
     6841 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgg gcccagggcc
     6901 gggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctcga attccttgtc
     6961 atcgtcatcc ttgtaatctc ggtaacctga accgatggtg tcctggtcct gcgcggatgt
     7021 caggtggggc ggctgacctg ctgccggggt ggatttcttt atcttctggc ccggagaagg
     7081 gcaggctggg atgtctgggg cctcgggggt actggggctg gaaacctcgt ccaccgacag
     7141 ccgcagtctc ctgcgggccg gtggcctggc ctggtcgcgt ccgggggcgg cgggcgggcg
     7201 ggcgcgcttg cggctgccac tggtagcgga ggccggggcg cggagtgcgg gcctggcggg
     7261 gctgggctcg agcggccgcc agtgtgatgg atatgtcgac ccattaagct tgtgccccag
     7321 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
     7381 gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt
     7441 gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct
     7501 cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt cagcggggta
     7561 cagcgtctcg gtgaaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga
     7621 tgtgaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt
     7681 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa
     7741 gccctcaggg aaggactgct tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt
     7801 cttgctgccg tagaggaagc tagtagccag gatgtcgaag gcgaagggga gagggccgcc
     7861 ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga
     7921 tgtgcacttg aagtgatggt tgtccacggt gccctccatg tacagcttca tgtgcatgtt
     7981 ctccttaatc agctcgctca taccggtgga tctcgacctg cagcccaagc ttcgtctaac
     8041 aaaaaagcca aaaacggcca gaatttagcg gacaatttac tagtctaaca ctgaaaatta
     8101 catattgacc caaatgatta catttcaaaa ggtgcctaaa aaacttcaca aaacacactc
     8161 gccaaccccg agcgcatagt tcaaaaccgg agcttcagct acttaagaag ataggtacat
     8221 aaaaccgacc aaagaaactg acgcctcact tatccctccc ctcaccagag gtccggcgcc
     8281 tgtcgattca ggagagccta ccctaggccc gaaccctgcg tcctgcgacg gagaaaagcc
     8341 taccgcacac ctaccggcag gtggccccac cctgcattat aagccaacag aacgggtgac
     8401 gtcacgacac gacgagggcg cgcgctccca aaggtacggg tgcactgccc aacggcaccg
     8461 ccataactgc cgcccccgca acagacgaca aaccgagttc tccagtcagt gacaaacttc
     8521 acgtcagggt ccccagatgg tgccccagcc catctcaccc gaataagagc tttcccgcat
     8581 tagcgaaggc ctcaagacct tgggttcttg ccgcccacca tgccccccac cttgtttcaa
     8641 cgacctcaca gcccgcctca caagcgtctt ccattcaaga ctcgggaaca gccgccattt
     8701 tgctgcgctc cccccaaccc ccagttcagg gcaaccttgc tcgcggaccc agactacagc
     8761 ccttggcggt ctctccacac gcttccgtcc caccgagcgg cccggcggcc acgaaagccc
     8821 cggccagccc agcagcccgc tactcaccaa gtgacgatca cagcgatcca caaacaagaa
     8881 ccgcgaccca aatcccggct gcgacggaac tagctgtgcc acacccggcg cgtccttata
     8941 taatcatcgg cgttcaccgc cccacggaga tccctccgca gaatcgccga gaagggacta
     9001 cttttcctcg cctgttccgc tctctggaaa gaaaaccagt gccctagagt cacccaagtc
     9061 ccgtcctaaa atgtccttct gctgatactg gggttctaag gccgagtctt atgagcagcg
     9121 ggccgctgtc ctgagcgtcc gggcggaagg atcaggacgc tcgctgcgcc cttcgtctga
     9181 cgtggcagcg ctcgccgtga ggaggggggc gcccgcggga ggcgccaaaa cccggcgcgg
     9241 aggccagatc tggagccgac acgggttaat caagcttctc gaggctagtc tcgtgatcga
     9301 taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     9361 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
     9421 ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     9481 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     9541 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     9601 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     9661 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     9721 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     9781 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     9841 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     9901 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     9961 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
    10021 ccctcagcaa attgttctgc tgctgcacta taccagacaa ta
//
