LOCUS       sequence-430584-       13929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic BiFC vector (Gateway compatible) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 13929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-N-GW-P2A:mCherry
FEATURES             Location/Qualifiers
     source          1..13929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1163..1165,1166..1168,1169..1681)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1469..1488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1682..1711
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1730..1854
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2283..2588)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2385..2404)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2908..3591)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     3506..3525
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3592..3694)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3872..3996)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4004..4060
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4061..4081
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4082..4102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4103..4123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4124..4834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4537..4556
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4851..5103
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5136..5152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5136..5152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5149..5171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5160..5176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5184..5190,5191..5208,5209..5214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5229..5250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5286..5538)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5689..6714)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6766..6945)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7451..7475
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7996..8787
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9033..9621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9522..9541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9775..9792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9807..9947
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9873..9892)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10033..10055
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10291..10485
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10551..11624)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12053..12682)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1261 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1621 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1681 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagta caagtttgta
     1741 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1801 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatgaatcaa
     1861 ctacttagat ggtattagtg acctgtagtc gaccgacagc cttccaaatg ttcttcgggt
     1921 gatgctgcca acttagtcga ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg
     1981 tatccagcct actcgctatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa
     2041 aagaggtgcg agcctctttt ttgtgtgaca aaataaaaac atctacctat tcatatacgc
     2101 tagtgtcata gtcctgaaaa tcatctgcat caagaacaat ttcacaactc ttatactttt
     2161 ctcttacaag tcgttcggct tcatctggat tttcagcctc tatacttact aaacgtgata
     2221 aagtttctgt aatttctact gtatcgacct gcagactggc tgtgtataag ggagcctgac
     2281 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
     2341 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
     2401 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
     2461 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
     2521 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
     2581 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
     2641 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
     2701 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
     2761 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
     2821 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
     2881 gcaaatacgc atactgttat ctggctttta gtaagccgga tccacgcggc gtttacgccc
     2941 cccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     3001 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     3061 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     3121 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     3181 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     3241 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     3301 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     3361 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     3421 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     3481 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     3541 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     3601 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     3661 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     3721 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     3781 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tagtcgacta
     3841 caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca
     3901 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3961 ttacgtttct cgttcagctt tcttgtacaa agtggttctc gaggcaacca actttagcct
     4021 gctcaagcaa gcaggagatg ttgaggaaaa tcctggcccc cctaagaaaa agagaaaggt
     4081 tccgaaaaag aaaagaaagg tgccaaaaaa aaagagaaag gtaatggtga gcaagggcga
     4141 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     4201 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4261 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     4321 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     4381 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4441 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     4501 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4561 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4621 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     4681 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     4741 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     4801 ctccaccggc ggcatggacg agctgtacaa gtaagagctc gaatttcccc gatcgttcaa
     4861 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     4921 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     4981 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5041 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5101 atcgggaatt ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     5161 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5221 ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa
     5281 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     5341 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     5401 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     5461 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     5521 ccaaatgttt gaacgatcgg ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa
     5581 ctgtgccacc aagcgtaagg ccgttctctc gcatttgcct tgctaggctc gcgcgagttg
     5641 ctggctgagg cgttctcgaa atcagctctt gttcggtcgg catctactct attcctttgc
     5701 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     5761 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     5821 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     5881 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     5941 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     6001 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     6061 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     6121 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     6181 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     6241 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     6301 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     6361 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     6421 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc
     6481 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     6541 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     6601 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     6661 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctta
     6721 ttgccccccg ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag
     6781 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     6841 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     6901 actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     6961 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     7021 tcatgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     7081 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     7141 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     7201 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     7261 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     7321 ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc
     7381 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     7441 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     7501 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     7561 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     7621 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     7681 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     7741 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     7801 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     7861 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     7921 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     7981 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     8041 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     8101 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     8161 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     8221 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     8281 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     8341 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     8401 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     8461 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     8521 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     8581 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     8641 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     8701 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     8761 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     8821 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     8881 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     8941 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9001 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9061 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     9181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9781 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     9841 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9901 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     9961 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    10021 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10081 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
    10141 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
    10201 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
    10261 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
    10321 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
    10381 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
    10441 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
    10501 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
    10561 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
    10621 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
    10681 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
    10741 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
    10801 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
    10861 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
    10921 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
    10981 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
    11041 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
    11101 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
    11161 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
    11221 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
    11281 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
    11341 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    11401 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    11461 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    11521 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    11581 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    11641 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
    11701 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    11761 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    11821 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    11881 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    11941 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    12001 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    12061 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    12121 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    12181 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    12241 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    12301 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    12361 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    12421 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    12481 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    12541 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    12601 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    12661 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    12721 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    12781 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    12841 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    12901 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    12961 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    13021 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    13081 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    13141 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    13201 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    13261 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    13321 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    13381 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    13441 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    13501 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    13561 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    13621 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    13681 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    13741 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    13801 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    13861 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    13921 gataaacct
//
