LOCUS       sequence-430592-        7739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under 
            synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Park J, Berthoux C, Hoyos-Ramirez E, Shan L, Morimoto-Tomita M, Wang
            Y, Castillo PE, Tomita S
  TITLE     Chemogenetic regulation of the TARP-lipid interaction mimics LTP and
            reversibly modifies behavior.
  JOURNAL   Cell Rep. 2023 Aug 29;42(8):112826. doi: 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19.
  PUBMED    37471228
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-08-29"; volume: "42(8):112826. doi"; pages: " 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19"
COMMENT     Sequence Label: pAAV-Syn-ExSYTE.H64A
FEATURES             Location/Qualifiers
     source          1..7739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     131..150
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    386..410
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1087..1800,1801..1803,1804..1806))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1090..1800,1801..1803,1804..1806))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1132..1153)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1481..1500
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1740..1761
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1807..1863)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4161..4185)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4207..4654)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   4684..4813
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(4875..5463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4955..4974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5634..6425,6426..6494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6257..6276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6495..6599)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6667..6685
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6723..6745)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6845..6864
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6881..7336)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7018..7039)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7230..7249
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   7411..7551
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7422..7551
                     /label=AAV2 ITR
     polyA_signal    join(7612..7739,1..349)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
ORIGIN
        1 aaaaaaacaa aaattagctg agcctggtca tgcatgcctg gaatcccaac aactcgggag
       61 gctgaggcag gagaatcgct tgaacccagg aggcggagat tgcagtgagc caagattgtg
      121 ccactgcact ccagcttggt tcccaataga ccccgcaggc cctacaggtt gtcttcccaa
      181 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
      241 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      301 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg tagatctctc
      361 gagcagcgct gctcgaggca agcttaccac tttgtacaag aaagctgggt gtctagaaga
      421 cacgagcagt taccaacacg aggtggttgg tgctggggtg tggatgaggt tccagctggg
      481 caatcacccg gcaggtcagg gaagtgcctg gaacagccta ggagccaagg catggggtct
      541 cctgggactg gacttgacca gcaggcaggg cacagcaggt aaggtcaggc tgggctaggg
      601 cccgaacctt aaggctcctg caaggttctg gcgggggcca aaggccacgc agcgaactgg
      661 gctctgtgct gagagggcac agaccctgct caagctgcag tggcttgtgg caggcagctg
      721 gtgagtggcc agcggagcgg gcattcgtgg cagacccggg gtttcacgct ggcttgtctt
      781 caccttcagc tctgaggctg agctgggtgc tgccctctgc caggcacctc ctctttgtaa
      841 tcctattttc tctgccttga aagtgtcttg gaacctctgt gcaggcagct tcaagagagg
      901 aggggacggt gctggtgctg gggtggtacc ccttccagac atggccggaa agacttctca
      961 gcagcttgag acctggtgtc attctcctgg ctctgaagac tccagctgct gcctcagctg
     1021 gggctgagga ctcagcccct ctgggaccct ccgcctggca tggctgagtc atggagccga
     1081 tctagactac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     1141 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
     1201 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1261 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     1321 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     1381 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     1441 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     1501 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1561 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     1621 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     1681 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     1741 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     1801 caccataggt ccagggttct cctccacgtc tccagcctgc ttcagcaggc tgaagttagt
     1861 agctccgctt cccacgggcg tggttttcct gttgagcgtg ttggtgttgg acgccgcggc
     1921 ctctttggac agggtcccgg gcgcgggcgc tgcaggagcg ggcggcgcgg gcgcgggcgc
     1981 gggtgcagcg ggcggtccgg tgactgtgac cgtgacgccg gacgccgctt ccttggggaa
     2041 ggcgttgtgc agcgtgagaa aacccgcact agtgctgccg ctttcgcact ttagctgttt
     2101 ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg ctccctgatg
     2161 gtcgaacagc tcaattgctt gtctcagaag tgggggcata gaatcggtgg taggtgtctc
     2221 tctttcctct tttgctactt gatgctcctg atcctccaat acgcagccca gtgtaaagtg
     2281 gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct gacacaggaa
     2341 cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc cgagatgcac
     2401 tttagccccg tcgcgatgtg agaggagagc acagcggaat gacttggcgt tgttccgcag
     2461 aaagtcttgc catgactcgc cttccagggg gcagaagtgg gtagcatgcc tgtccagcat
     2521 ctcgattgcc agggcatcga gcagggcccg cttgttcttc acgtgccagt acagggtagg
     2581 ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga taccgacttc
     2641 attgagtaat tccagagcag agtttatgac tttgctcttg tccagtctag acatgctctt
     2701 ccggaggaat tttctcagcc tcttccaaaa tttgccgctg ccgctttcgc actttagctg
     2761 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg
     2821 atggtcgaac agctcaattg cttgtctcag aagtgggggc atagaatcgg tggtaggtgt
     2881 ctctctttcc tcttttgcta cttgatgctc ctgatcctcc aatacgcagc ccagtgtaaa
     2941 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag
     3001 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg
     3061 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg aatgacttgg cgttgttccg
     3121 cagaaagtct tgccatgact cgccttccag ggggcagaag tgggtagcat gcctgtccag
     3181 catctcgatt gccagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt
     3241 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac
     3301 ttcattgagt aattccagag cagagtttat gactttgctc ttgtccagtc tagacattgc
     3361 ctcgcggctg cgctcgctcg aggggccgct cccgccactg ccgcccgcgc cgccgccggc
     3421 cttgagcagg tccgagcgtg attggcagtg tgcctcgcgg ctgcgctcga tgtagatgtt
     3481 gacggccagt actccgatca cctcagccag gatgaaggac agcccgccga agtagaagga
     3541 ccagccgtac gagtagtggt ttttcttctc ctcgtccctc ttggggccag gctcgccggc
     3601 gttggccgat atgtacacaa tcaccccgat gatgttgctc aggcctgctg ccacgaacag
     3661 gatccctgcg cccaggatga tgttcctttt ggatttgtag acgcgagagg cagctacgca
     3721 cacgcccccg agcagcagca ggatggcgct caggatagga aagatgctgg aggcccggac
     3781 tactcggagc aggtactccg cgctgtcgtg gtcgtagtcc gtgtcctccg ggaagtggtt
     3841 gatcttcacg cagacacctc ttttcaaccc ttccaggcag catatccgcc agaggcctga
     3901 atgtgtgagg cccccagggt ccttcttctc tgaggagcca ctgcccccac gatggggtgg
     3961 tccatcatca cctgctgtga ggttggtggt gttgcagatg agagctcttg tgtagagcca
     4021 gtagtcagtg ctgatggcga tggtcatgag gccaaaagct gcaaaggcgc ctatggtggt
     4081 cagtagtacc tgaacgccct tttcgcacca caaccccctc tcttcattcc agcgtttcaa
     4141 tgactccatt ttaagcttgg agcctgcttt tttgtacaaa cttgtagatc cccggggaat
     4201 tctcgactgc gctctcaggc acgacacgac tcctccgctg cccaccgcag actgaggcag
     4261 cgctgagtcg ccggcgccgc agcgcagatg gtcgcgcccg tgccccccta tctcgcgcct
     4321 cgcgtggtgc ggtccggctg ggccggcggc ggcgcggacg cgaccaaggt ggccgggaag
     4381 gggagtttgc gggggaccgg cgagtgacgt cagcgcgcct tcagtgctga ggcggcggtg
     4441 gcgcgcgccg ccaggcgggg gcgaaggcac tgtccgcggt gctgaagctg gcagtgcgca
     4501 cgcgcctcgc cgcatcctgt ttcccctccc cctctctgat aggggatgcg caatttgggg
     4561 aatgggggtt gggtgcttgt ccagtgggtc ggggtcggtc gtcaggtagg cacccccacc
     4621 ccgcctcatc ctggtcctaa aacccacttg cactcatacg cagggccctc tgcaggcggc
     4681 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4741 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
     4801 agctgcctgc aggacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4861 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4921 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4981 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5041 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5101 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5161 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5221 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5281 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5341 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5401 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5461 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5521 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5581 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5641 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5701 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5761 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5821 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5881 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5941 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6001 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6061 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6121 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6181 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6241 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6301 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6361 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6421 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6481 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6541 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6601 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     6661 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6721 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6781 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6841 tatgcggcat cagagcagat tgtactgaga gtgcaccata aaattgtaaa cgttaatatt
     6901 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca atagaccgaa
     6961 atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagagttgag tgttgttcca
     7021 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     7081 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
     7141 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     7201 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctaag
     7261 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7321 ccgctacagg gcgcgtacta tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat
     7381 gcgtaaggag aaaataccgc atcaggcgcc cctgcaggca gctgcgcgct cgctcgctca
     7441 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
     7501 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc tgcgtccgct
     7561 cggtgggttc ctgcgtccgc tcggtccgca cgtggttacc tacaaaatca gaaggacagg
     7621 gaagggagca gtggttcacg cctgtaatcc cagcaatttg ggaggccaag gtgggtagat
     7681 cacctgagat taggagttgg agaccagcct ggccaatatg gtgaaacccc gtctctacc
//
