LOCUS       sequence-430596-        5399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ExSYTE in oocyte vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Park J, Berthoux C, Hoyos-Ramirez E, Shan L, Morimoto-Tomita M, Wang
            Y, Castillo PE, Tomita S
  TITLE     Chemogenetic regulation of the TARP-lipid interaction mimics LTP and
            reversibly modifies behavior.
  JOURNAL   Cell Rep. 2023 Aug 29;42(8):112826. doi: 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19.
  PUBMED    37471228
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-08-29"; volume: "42(8):112826. doi"; pages: " 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19"
COMMENT     Sequence Label: pGEM-HE-ExSYTE
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(65..83)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(66..83)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(101..117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(101..117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(114..136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(149..155,156..173,174..179))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(332..349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(503..1091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(583..602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1262..2053,2054..2122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1885..1904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2123..2227)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2295..2313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2351..2373)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2473..2492
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2686..2708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2701..2717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2724..2743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2724..2742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2774..2816
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     protein_bind    2839..2863
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5210..5234)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     3'UTR           5257..5382
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 aaaaaaaaaa aaaaaccccc cccccccccc ctgcaggcat gcaagctagc accggtttga
       61 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      181 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      241 tccagtcggg aaacttgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2221 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2401 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2461 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     2521 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     2581 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     2641 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     2701 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaatta attcgagctc
     2761 ggtacccagc ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga
     2821 tcaattcccc ggggatctac aagtttgtac aaaaaagcag gctccaagct taaaatggag
     2881 tcattgaaac gctggaatga agagaggggg ttgtggtgcg aaaagggcgt tcaggtacta
     2941 ctgaccacca taggcgcctt tgcagctttt ggcctcatga ccatcgccat cagcactgac
     3001 tactggctct acacaagagc tctcatctgc aacaccacca acctcacagc aggtgatgat
     3061 ggaccacccc atcgtggggg cagtggctcc tcagagaaga aggaccctgg gggcctcaca
     3121 cattcaggcc tctggcggat atgctgcctg gaagggttga aaagaggtgt ctgcgtgaag
     3181 atcaaccact tcccggagga cacggactac gaccacgaca gcgcggagta cctgctccga
     3241 gtagtccggg cctccagcat ctttcctatc ctgagcgcca tcctgctgct gctcgggggc
     3301 gtgtgcgtag ctgcctctcg cgtctacaaa tccaaaagga acatcatcct gggcgcaggg
     3361 atcctgttcg tggcagcagg cctgagcaac atcatcgggg tgattgtgta catatcggcc
     3421 aacgccggcg agcctggccc caagagggac gaggagaaga aaaaccacta ctcgtacggc
     3481 tggtccttct acttcggcgg gctgtccttc atcctggctg aggtgatcgg agtactggcc
     3541 gtcaacatct acatcgagcg cagccgcgag gcacactgcc aatcacgctc ggacctgctc
     3601 aaggccggcg gcggcgcggg cggcagtggc gggagcggcc cctcgagcga gcgcagccgc
     3661 gaggcaatgt ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat
     3721 gaagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     3781 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct ggcaatcgag
     3841 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     3901 ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa
     3961 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     4021 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     4081 cactttacac tgggctgcgt attggaggat caggagcatc aagtagcaaa agaggaaaga
     4141 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     4201 catcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     4261 aaacagctaa agtgcgaaag cggcagcggc aaattttgga agaggctgag aaaattcctc
     4321 cggaagagca tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc
     4381 aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     4441 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctggcaatc
     4501 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     4561 tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg cgacggggct
     4621 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     4681 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     4741 ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc aaaagaggaa
     4801 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     4861 gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     4921 gagaaacagc taaagtgcga aagcggcagc actagtgcgg gttttctcac gctgcacaac
     4981 gccttcccca aggaagcggc gtccggcgtc acggtcacag tcaccggacc gcccgctgca
     5041 cccgcgcccg cgcccgcgcc gcccgctcct gcagcgcccg cgcccgggac cctgtccaaa
     5101 gaggccgcgg cgtccaacac caacacgctc aacaggaaaa ccacgcccgt gtagagatct
     5161 gatgtaaatc actagtgcgg ccgcctgcag gtcgagcatg catctagaca cccagctttc
     5221 ttgtacaaag tggtaagctt gatctggtta ccactaaacc agcctcaaga acacccgaat
     5281 ggagtctcta agctacataa taccaactta cactttacaa aatgttgtcc cccaaaatgt
     5341 agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa aaaaaaaaa
//
