LOCUS       sequence-430611-        8231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ExSYTE - EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Park J, Berthoux C, Hoyos-Ramirez E, Shan L, Morimoto-Tomita M, Wang
            Y, Castillo PE, Tomita S
  TITLE     Chemogenetic regulation of the TARP-lipid interaction mimics LTP and
            reversibly modifies behavior.
  JOURNAL   Cell Rep. 2023 Aug 29;42(8):112826. doi: 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19.
  PUBMED    37471228
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-08-29"; volume: "42(8):112826. doi"; pages: " 
            10.1016/j.celrep.2023.112826. Epub 2023 Jul 19"
COMMENT     Sequence Label: pGW1-ExSYTE-EGFP
FEATURES             Location/Qualifiers
     source          1..8231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     161..177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        743..1122
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1123..1326
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1277..1297
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1323..1347
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1462..2288
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     protein_bind    2312..2336
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4676..4678,4679..4681,4682..5395)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4676..4678,4679..4681,4682..5392)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4721..4742)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4982..5001)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5329..5350
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5403..5427)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5561..5695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5598..5617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5652..5671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5718..5734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5718..5734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5731..5753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5742..5758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5766..5772,5773..5790,5791..5796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5948..5965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6119..6707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6199..6218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6878..7669,7670..7738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7501..7520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7948..7966
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8004..8026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8126..8145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaaaaaatt agtcagggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
       61 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      181 cgtgtcaagg acggtgactg cagtgaataa taaaatgtgt gtttgtccga aatacgcgtt
      241 ttgagatttc tgtcgccgac taaattcatg tcgcgcgata gtggtgttta tcgccgatag
      301 agatggcgat attggaaaaa tcgatatttg aaaatatggc atattgaaaa tgtcgccgat
      361 gtgagtttct gtgtaactga tatcgccatt tttccaaaag tgatttttgg gcatacgcga
      421 tatctggcga tagcgcttat atcgtttacg ggggatggcg atagacgact ttggtgactt
      481 gggcgattct gtgtgtcgca aatatcgcag tttcgatata ggtgacagac gatatgaggc
      541 tatatcgccg atagaggcga catcaagctg gcacatggcc aatgcatatc gatctataca
      601 ttgaatcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
      661 ggctattggc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
      721 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      781 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      841 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      901 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      961 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1021 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1081 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1141 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1201 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1261 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1321 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1381 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
     1441 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
     1501 gcttcttatg catgctatac tgtttttggc ttggggtcta tacacccccg cttcctcatg
     1561 ttataggtga tggtatagct tagcctatag gtgtgggtta ttgaccatta ttgaccactc
     1621 ccctattggt gacgatactt tccattacta atccataaca tggctctttg ccacaactct
     1681 ctttattggc tatatgccaa tacactgtcc ttcagagact gacacggact ctgtattttt
     1741 acaggatggg gtctcattta ttatttacaa attcacatat acaacaccac cgtccccagt
     1801 gcccgcagtt tttattaaac ataacgtggg atctccacgc gaatctcggg tacgtgttcc
     1861 ggacatgggc tcttctccgg tagcggcgga gcttctacat ccgagccctg ctcccatgcc
     1921 tccagcgact catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg
     1981 cacagcacga tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg
     2041 tctgaaaatg agctcgggga gcgggcttgc accgctgacg catttggaag acttaaggca
     2101 gcggcagaag aagatgcagg cagctgagtt gttgtgttct gataagagtc agaggtaact
     2161 cccgttgcgg tactgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc
     2221 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt
     2281 ttctgcagtc accgtccttg acacgaagct tacaagtttg tacaaaaaag caggctccaa
     2341 gcttaaaatg gagtcattga aacgctggaa tgaagagagg gggttgtggt gcgaaaaggg
     2401 cgttcaggta ctactgacca ccataggcgc ctttgcagct tttggcctca tgaccatcgc
     2461 catcagcact gactactggc tctacacaag agctctcatc tgcaacacca ccaacctcac
     2521 agcaggtgat gatggaccac cccatcgtgg gggcagtggc tcctcagaga agaaggaccc
     2581 tgggggcctc acacattcag gcctctggcg gatatgctgc ctggaagggt tgaaaagagg
     2641 tgtctgcgtg aagatcaacc acttcccgga ggacacggac tacgaccacg acagcgcgga
     2701 gtacctgctc cgagtagtcc gggcctccag catctttcct atcctgagcg ccatcctgct
     2761 gctgctcggg ggcgtgtgcg tagctgcctc tcgcgtctac aaatccaaaa ggaacatcat
     2821 cctgggcgca gggatcctgt tcgtggcagc aggcctgagc aacatcatcg gggtgattgt
     2881 gtacatatcg gccaacgccg gcgagcctgg ccccaagagg gacgaggaga agaaaaacca
     2941 ctactcgtac ggctggtcct tctacttcgg cgggctgtcc ttcatcctgg ctgaggtgat
     3001 cggagtactg gccgtcaaca tctacatcga gcgcagccgc gaggcacact gccaatcacg
     3061 ctcggacctg ctcaaggccg gcggcggcgc gggcggcagt ggcgggagcg gcccctcgag
     3121 cgagcgcagc cgcgaggcaa tgtctagact ggacaagagc aaagtcataa actctgctct
     3181 ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     3241 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     3301 cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     3361 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     3421 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     3481 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     3541 gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc
     3601 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     3661 tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     3721 atgtggcctg gagaaacagc taaagtgcga aagcggcagc ggcaaatttt ggaagaggct
     3781 gagaaaattc ctccggaaga gcatgtctag actggacaag agcaaagtca taaactctgc
     3841 tctggaatta ctcaatgaag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     3901 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     3961 tgccctggca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     4021 gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc tcctctcaca
     4081 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     4141 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     4201 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggatcagg agcatcaagt
     4261 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     4321 aattgagctg ttcgaccatc agggagccga acctgccttc cttttcggcc tggaactaat
     4381 catatgtggc ctggagaaac agctaaagtg cgaaagcggc agcactagtg cgggttttct
     4441 cacgctgcac aacgccttcc ccaaggaagc ggcgtccggc gtcacggtca cagtcaccgg
     4501 accgcccgct gcacccgcgc ccgcgcccgc gccgcccgct cctgcagcgc ccgcgcccgg
     4561 gaccctgtcc aaagaggccg cggcgtccaa caccaacacg ctcaacagga aaaccacgcc
     4621 cgtgggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggagatggt
     4681 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4741 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4801 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4861 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     4921 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4981 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5041 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5101 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     5161 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5221 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5281 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5341 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagtctag
     5401 acacccagct ttcttgtaca aagtggtctc gacctgcagg atatcgaatt cattgatcat
     5461 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5521 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5581 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5641 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgtac
     5701 cgagctcgcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5761 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5821 taactacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5881 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5941 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6001 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6061 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6121 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6181 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6241 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6301 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6361 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6421 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6481 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6541 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6601 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6661 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6721 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6781 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6841 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6901 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6961 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7021 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7081 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7141 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7201 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7261 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7321 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7381 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7441 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7501 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7561 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7621 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7681 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7741 tcttcctttt tcaataagcg gccgcggcca tgccggccac tagtctcgag ttattattga
     7801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     7921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     7981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8161 atgaagacgt cgcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     8221 tctgcataaa t
//
