LOCUS       sequence-430621-       15261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Variant of lentiCRISPRv2 that confers hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15261)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 15261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-20"; volume: "9(1):4902. doi"; pages: " 
            10.1038/s41598-019-41277-z"
COMMENT     Sequence Label: lentiCRISPRv2 hygro
FEATURES             Location/Qualifiers
     source          1..15261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             569..616
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             617..640
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             650..706
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1703..2291
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1756..1776)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2174..2185)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2363..2596
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2622..2639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2628..2852
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2898..3326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2985..3004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3195..3216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3335..3355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3340..3669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3520..3655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3582..3601
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3717..3764
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3783..4157
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4287..4408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4324..4343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4378..4397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4457..4473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4457..4473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4470..4492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4481..4497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4505..4511,4512..4529,4530..4535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4550..4571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4688..4705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4859..5447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4939..4958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5618..6409,6410..6478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6241..6260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6479..6583)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6658..6677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6853..7232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7234..7432
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7387..7407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7450..7630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7677..7802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8295..8528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8713..8757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9055..9172
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9223..9471
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9223..9243
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9394..9413
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        11353..11428
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        11490..11701
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      11720..11729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11726..15261,1..568)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(11726..15261,1..568)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
       61 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
      121 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
      181 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
      241 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
      301 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
      361 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
      421 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
      481 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
      541 gatcgacctg tctcagctgg gaggcgacaa gcgacctgcc gccacaaaga aggctggaca
      601 ggctaagaag aagaaagatt acaaagacga tgacgataag ggatccggcg caacaaactt
      661 ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgaaaa agcctgaact
      721 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt ccgacctgat
      781 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
      841 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
      901 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
      961 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     1021 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     1081 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     1141 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     1201 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     1261 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     1321 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     1381 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     1441 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     1501 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     1561 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     1621 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtacttcg
     1681 ccgatagacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     1741 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1801 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1861 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1921 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1981 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2041 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2101 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2161 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2221 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2281 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2341 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     2401 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2461 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2521 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2581 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     2641 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2701 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2761 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2821 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     2881 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2941 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3001 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3061 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3121 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3181 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3241 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3301 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3361 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3421 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3481 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3541 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3601 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3661 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     3721 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     3781 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     3841 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     3901 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     3961 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     4021 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     4081 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     4141 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     4201 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4261 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     4321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4381 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     4441 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5281 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6601 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     6661 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     6721 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     6781 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     6841 tacgcgcgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     6901 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     6961 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7021 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7081 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7141 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7201 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7261 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7321 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7381 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
     7441 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7621 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7681 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7741 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7801 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7861 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7921 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7981 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8041 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8101 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     8161 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     8221 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     8281 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     8341 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8401 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8461 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8521 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8581 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8641 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8701 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8761 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8821 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8881 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     8941 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     9001 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     9061 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9121 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     9181 ttacagggac agcagagatc cagtttggtt aattaaggta ccgagggcct atttcccatg
     9241 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     9301 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     9361 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     9421 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacggt
     9481 tgtaaatgag cacacaaaat acacatgcta aaatattata ttctatgacc tttataaaat
     9541 caaccaaaat cttcttttta ataactttag tatcaataat tagaattttt atgttccttt
     9601 ttgcaaactt ttaataaaaa tgagcaaaat aaaaaaacgc tagttttagt aactcgcgtt
     9661 gttttcttca cctttaataa tagctactcc accacttgtt cctaagcggt cagctcctgc
     9721 ttcaatcatt ttttgagcat cttcaaatgt tctaactcca ccagctgctt taactaaagc
     9781 attgtcttta acaactgact tcattagttt aacatcttca aatgttgcac ctgattttga
     9841 aaatcctgtt gatgttttaa caaattctaa tccagcttca acagctattt cacaagcttt
     9901 catgatttct tcttttgtta ataaacaatt ttccataata catttaacaa catgtgatcc
     9961 agctgctttt tttacagctt tcatgtcttc taaaactaat tcataatttt tgtcttttaa
    10021 tgcaccaata tttaatacca tatcaatttc tgttgcacca tctttaattg cttcagaaac
    10081 ttcgaatgct tttgtagctg ttgtgcatgc acctagagga aaacctacaa catttgttat
    10141 tcctacattt gtgcctttta ataattcttt acaatagctt gttcaatatg aattaacaca
    10201 aactgttgca aaatcaaatt caattgcttc atcacataat tgtttaattt cagctttcgt
    10261 agcatcttgt tttaataatg tgtgatctat atatttgttt agtttcattt tttctcctat
    10321 atattcattt ttaattttaa ttctttaata atttcgtcta ctttaacttt agcgttttga
    10381 acagattcac caacacctat aaaataaatt tttagtttag gttcagttcc acttgggcga
    10441 acagcaaatc atgacttatc ttctaaataa aattttagta agtcttgtcc tggcatatta
    10501 tacattccat cgatgtagtc ttcaacatta acaactttaa gtccagcaat ttgagttaag
    10561 ggtgttgctc tcaatgattt cattaatggt tcaattttta atttcttttc ttctggttta
    10621 aaattcaagt ttaaagtgaa agtgtaatat gcacccattt ctttaaataa atcttctaaa
    10681 tagtctacta atgttttatt ttgtttttta taaaatcaag cagcctctgc tattaatata
    10741 gaagcttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc ataactttct
    10801 tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc tctacccatt
    10861 catttaaatc cagttaaagt ttttacaata ttaactccat atttttcatg agcgattcta
    10921 tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg aatgctattt
    10981 aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg atttccatct
    11041 aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc atctgggtca
    11101 ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat ttttcatgca
    11161 ggatcaaatt ctggatttgg atttacaaca tttttaaatg tttcatcttc aaatgcatgc
    11221 tcttcaacct caataacgtt atatcctgat tcacgtaata tttttggggt aaatttagtt
    11281 cctgttccat taactgcgct aaaaataatt tttaaatctt ttttagcttc ttgctctttt
    11341 ttgtacgtct ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    11401 acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc gctagctagg tcttgaaagg
    11461 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
    11521 gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa
    11581 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
    11641 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
    11701 ggaccggttc tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg
    11761 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
    11821 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
    11881 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
    11941 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
    12001 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
    12061 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
    12121 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
    12181 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
    12241 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
    12301 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
    12361 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
    12421 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
    12481 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
    12541 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
    12601 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
    12661 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
    12721 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
    12781 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
    12841 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
    12901 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
    12961 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
    13021 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
    13081 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
    13141 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
    13201 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
    13261 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
    13321 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
    13381 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
    13441 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
    13501 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
    13561 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
    13621 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
    13681 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
    13741 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
    13801 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
    13861 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
    13921 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
    13981 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
    14041 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
    14101 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
    14161 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
    14221 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
    14281 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
    14341 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
    14401 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
    14461 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
    14521 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
    14581 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
    14641 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
    14701 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
    14761 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
    14821 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
    14881 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
    14941 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
    15001 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
    15061 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
    15121 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
    15181 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
    15241 cagcttcgag aagaatccca t
//
