LOCUS       sequence-430657-        5022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  recombinant AAV vector packaging self-complementary Renilla 
            Luciferase under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Paulk NK, Pekrun K, Charville GW, Maguire-Nguyen K, Wosczyna MN, Xu 
            J, Zhang Y, Lisowski L, Yoo B, Vilches-Moure JG, Lee GK, Shrager JB,
            Rando TA, Kay MA
  TITLE     Bioengineered Viral Platform for Intramuscular Passive Vaccine 
            Delivery to Human Skeletal Muscle.
  JOURNAL   Mol Ther Methods Clin Dev. 2018 Jul 24;10:144-155. doi: 
            10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21.
  PUBMED    30101152
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2018-07-24"; volume: "10:144-155. doi"; 
            pages: " 10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21"
COMMENT     Sequence Label: pscAAV-CAG-RLuc
FEATURES             Location/Qualifiers
     source          1..5022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        417..697
                     /label=chicken beta-actin promoter
     primer_bind     976..998
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1060..1079
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1118..2053
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     2108..2127
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2377..2394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2377..2393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2386..2408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2602..2621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2721..2743
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2781..2799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2867..2971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2972..3040,3041..3832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3190..3209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4003..4591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4492..4511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4879..4900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4915..4920,4921..4938,4939..4945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4953..4969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4958..4980
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4977..4993
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4977..4993
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaagcttccc ggggggatct gggccactcc ctctctgcgc gctcgctcgc tcactgaggc
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc ctggaggggt ggagtcgtga cctaggccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
      421 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      481 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggggg
      541 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      601 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      661 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
      721 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      781 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
      841 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
      901 ccctttgtgc ggggggagcg gctcggggct gtccgcgggg ggacggctgc cttcgggggg
      961 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1021 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     1081 tgtctcatca ttttggcaaa gaattggatc cgccaccatg acttcgaaag tttatgatcc
     1141 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
     1201 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
     1261 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
     1321 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
     1381 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
     1441 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
     1501 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
     1561 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
     1621 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
     1681 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
     1741 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
     1801 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
     1861 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
     1921 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
     1981 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
     2041 aaatgaacaa taattctaga gcggccgctt cgagcagcct cacctgcgat ctcgatgctt
     2101 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2161 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2221 tttttaaact agtccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     2281 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga
     2341 gagggacaga tccgggcccg catgcgtcga caattcactg gccgtcgttt tacaacgtcg
     2401 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2461 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2521 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2581 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     2641 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2701 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2761 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2821 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2881 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2941 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3001 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3061 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3121 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3181 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3241 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3301 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3361 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3421 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3481 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3541 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3601 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3661 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3721 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3781 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3841 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3901 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3961 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4021 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4081 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4141 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4201 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4261 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4321 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4381 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4441 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4501 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4561 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4621 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4681 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4741 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4801 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4861 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4921 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4981 aaacagctat gaccatgatt acgccaagct ctcgagatct ag
//
