LOCUS       sequence-430689-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enables scarless gene deletions in Streptococcus pyogenes using the 
            counterselection marker pheS*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Lautenschlager N, Schmidt K, Schiffer C, Wulff TF, Hahnke K, 
            Finstermeier K, Mansour M, Elsholz AKW, Charpentier E
  TITLE     Expanding the genetic toolbox for the obligate human pathogen 
            Streptococcus pyogenes.
  JOURNAL   Front Bioeng Biotechnol. 2024 Jun 7;12:1395659. doi: 
            10.3389/fbioe.2024.1395659. eCollection 2024.
  PUBMED    38911550
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2024-06-7"; volume: "12:1395659. doi"; 
            pages: " 10.3389/fbioe.2024.1395659. eCollection 2024"
COMMENT     Sequence Label: pEC3135 (pERASE)
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaccttc aagctcaatt ggaagaactt aaaactaaaa ccttggaaac tcttcaaagt
       61 cttactggta accatacaaa agaattgcaa gatttgcgtg ttgcagttct tggtaagaag
      121 ggttcactta ctgatttgct taaaggtctt aaggatttgt caaacgattt gcgtcctgtg
      181 gtaggcaaac aagtaaacga agttcgtgac ttattgacaa aagctttcga agagcaagct
      241 aagatcgttg aagccgctaa aattcaagct caattggatg ctgaatcaat cgacgttact
      301 cttcctggac gtcaaatgac tctggggcat cgtcacgttc ttactcaaac atcagaagaa
      361 atcgaagaca tcttccttgg tatgggtttc caaattgtag atggtttcga agttgaaaaa
      421 gattattata acttcgaacg tatgaacctt ccaaaagacc accctgctcg tgacatgcaa
      481 gacactttct acattactga agagatcttg ttacgtactc acacttcacc agtacaagct
      541 cgtactcttg atcaacacga cttctctaag ggccctttaa aaatggtttc accaggccgt
      601 gtattccgcc gcgacactga cgacgctact cactcacacc aattccacca aatcgaaggt
      661 cttgtagttg gcaaaaatat ttcaatgggt gacttgaaag gcactttgga aatgatcatt
      721 aaaaaaatgt tcggtgacga acgtagcatc cgtcttcgcc cttcttattt ccctttctca
      781 gaaccatcag tagaagttga cgtatcatgt ttcaaatgtg gaggtaaagg ctgtaacgtt
      841 tgtaaaaaaa ctggttggat cgaaatcttg ggtgctggta tggttcaccc aagtgttctt
      901 gaaatgtcag gtgttgatgc aaaagagtac tcaggtttcg gtttcggctt gggacaagag
      961 cgtatcgcta tgttgcgcta cggaattaac gatattcgtg gcttctacca aggcgaccaa
     1021 cgtttctcag aacaatttaa ctaa
//
