LOCUS       sequence-430696-       13299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted removal of m6A on transcript
            at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under
            human Syn1 and U6 promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Madugalle SU, Liau WS, Zhao Q, Li X, Gong H, Marshall PR, 
            Periyakaruppiah A, Zajaczkowski EL, Leighton LJ, Ren H, Musgrove 
            MRB, Davies JWA, Kim G, Rauch S, He C, Dickinson BC, Fulopova B, 
            Fletcher LN, Williams SR, Spitale RC, Bredy TW
  TITLE     Synapse-Enriched m(6)A-Modified Malat1 Interacts with the Novel 
            m(6)A Reader, DPYSL2, and Is Required for Fear-Extinction Memory.
  JOURNAL   J Neurosci. 2023 Oct 25;43(43):7084-7100. doi: 
            10.1523/JNEUROSCI.0943-23.2023. Epub 2023 Sep 5.
  PUBMED    37669863
REFERENCE   2  (bases 1 to 13299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0943-23.2023"; journalName: "J Neurosci"; date: 
            "2023-10-25- 25"; volume: "43"; issue: "43"; pages: "7084-7100"
COMMENT     Sequence Label: CIRTS-FTO-control
FEATURES             Location/Qualifiers
     source          1..13299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3732..4439
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3877..3895)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             4449..4502
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5019..5045
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     RBS             5282..5290
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        7636..7884
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7636..7656
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7807..7826
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7991..8579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8044..8064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8462..8473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8582..8598)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8583..8599)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9104..9284
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9310..9327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9316..9540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9586..10014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9673..9692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9883..9904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10023..10043)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10028..10357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10208..10343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10270..10289
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10405..10452
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10471..10845
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10975..11096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11012..11031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11066..11085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11145..11161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11145..11161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11158..11180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11169..11185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11193..11199,11200..11217,11218..11223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11238..11259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11376..11393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11547..12135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11627..11646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12306..13097,13098..13166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12929..12948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13167..13271)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     3781 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     3841 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     3901 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     3961 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     4021 attgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     4081 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     4141 acggccccgt aatgcagtgt cgtaccatgg gctgggaggc ctccactgag cggatgtacc
     4201 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     4261 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     4321 cctacaacgt cgacatcaag ttggacatcc tttcccacaa cgaggactac accatcgtgg
     4381 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     4441 gctccggcga gggcagggga agtctactaa catgcgggga cgtggaggaa aatcccggcc
     4501 caaccggtat gggaattatc aacacccttc aaaaatacta ctgtcgcgtc cgtgggggtc
     4561 gttgcgcagt cctgtcgtgc ctgcctaaag aggagcagat cggtaaatgc tcaacccgtg
     4621 gacgtaagtg ctgtcgtcgc aagaaaggcg gttctggagg ctcagggggg agcggagggt
     4681 ccggtggatc aggaggctcc atggcagttc ccgagacccg ccctaaccac actatttata
     4741 tcaacaacct caatagcaag atcaagaagg atgagctaaa aaagtccctg tacgccatct
     4801 tctcccagtt tggccagatc ctggatatcc tggtaccgcg gcagcgtacc ccgaggggcc
     4861 aggcctttgt catcttcaag gaggtcagca gcgccaccaa cgccctgcgc tccatgcagg
     4921 gtttcccttt ctatgacaaa cctatgcgta tccagtatgc caagaccgac aaacgtatcc
     4981 cggccaagat gaaaggcacc ttcgtgggat cccttcaact gcctccactt gaaagactga
     5041 cactgggcgg tagtggagga agcgggggga gcggagggag tggtggatcc ggcggctcca
     5101 agcgcgtcca gaccgcggag gaacgagagc gggaagctaa gaaactgagg ctccttgagg
     5161 agcttgaaga cacttggctt ccttacctga cccccaaaga tgatgagttc tatcagcagt
     5221 ggcagctgaa ataccctaaa ctggttttcc gagaggccgg cagcatacca gaggagctgc
     5281 ataaggaggt ccccgaggcc tttctcacac tgcataagca tggctgcttg tttcgggacg
     5341 tggtgaggat ccaaggcaaa gatgtgctca ccccagtgtc tcgcatcctc atcggggacc
     5401 caggctgcac ctacaagtac ttgaacacca gactcttcac ggtgccctgg cccgtgaagg
     5461 gctgcacggt caagtacaca gaggctgaga tcgccgctgc atgtcagacc ttcctaaagc
     5521 tcaatgacta cctccaggtg gagaccatcc aggccttgga agaactggct gtcagagaga
     5581 aggccaatga agacgctgtg ccactgtgca tggcagagtt ccccagggcc ggcgtggggc
     5641 cgtcctgcga tgatgaagtg gaccttaaga gcagagcagc ctacaacgtg actttgctaa
     5701 acttcatgga tcctcagaag atgccctact tgaaagagga gccctatttc ggcatgggga
     5761 agatggcggt gagctggcat cacgatgaga acctggtgga caggtcagcc gtggcagtgt
     5821 acagctatag ctgcgaaggc tctgaggatg aaagtgagga cgagtccagc ttcgaaggca
     5881 gagatcctga tacttggcat gttggtttta agatctcttg ggacatcgag acaccaggat
     5941 taacaatccc tcttcaccag ggagactgct atttcatgct ggatgacctc aatgccaccc
     6001 accagcactg tgttttggct ggctcacagc ctcggtttag ttccactcac cgtgtggcag
     6061 agtgctcaac aggcaccttg gattatatct tagaacgctg tcagttggcg ctgcagaatg
     6121 tcctcaatga ctcagacgat ggcgacgtct cgttgaaatc ctttgatcct gcagttttga
     6181 aacaaggaga ggaaatccat aatgaggtgg agtttgagtg gctgaggcag ttctggtttc
     6241 aaggcaatcg atacaaactt tgcaccgatt ggtggtgtga gcccatgact cacctggagg
     6301 ggctgtggaa gaagatggag agcatgacaa atgcggtgct ccgtgaagtt aaaagagagg
     6361 ggctcccggt ggaacaaagg agtgagattc tgtctgccat cctggtcccg ctcaccgtgc
     6421 gccagaacct gaggaaggag tggcatgcca ggtgccagtc ccgagtcgtc cggactttac
     6481 cagtacagca gaaaccagac tgccggccat attgggagaa ggatgaccct tccatgcctc
     6541 tgccctttga cctcacagac gtggtttccg agctcagagg ccagctgctg gaagcaagat
     6601 cctaggctag cgggagcctc tgccctcctc ctgcagcccg gatggctctc ctctgttttt
     6661 ggtttgcttc cttttgtttg cccttctttt gggtgaccgg ggtggccacc attcctgtcc
     6721 ctgcccagtg gggaggggag caaggccttc tgccaagagg aggagcatgt cccctgccac
     6781 tgttgcatgc accccggccc tgtgactctg tgtgcaagcc cagcagcctg tggggcaggg
     6841 tgccaagagg ggacgggacg gacgttccag gacagaggga gtcgaggatg tgatccagga
     6901 cgggtggggg gtggaagggt gggaaagggc aggaaggcct tggaagctaa catgctttca
     6961 ctactggagg gggacggata gagcggatgg cactcgggtc tcactgacgc tgttctgaaa
     7021 ccatctggct ggctttctga ggtcaggctg ggtagggggc tgtcatcggc catactgctg
     7081 atcacacttg ccctcatccg tccagcgatc tcacgctgtt ctgtaaatac ctggtgctaa
     7141 catcccatgc cgctccccca tggcaccccc agcctcctgc ccagttcagg ctggtccagg
     7201 aatggatgtg ggggatggtc gctgtgtaac gtgctggtga tctttttccc ctaccccttt
     7261 tatgaccctt aaaactttga ttttgtccta aacatgccgg gccaactaaa gggtggggag
     7321 aggagagacg agccccacca tgctcacaag aaccaacctg caataaacac ttctgtgggc
     7381 cgccgcgcag agcacgcagc gggcacctcc tgcccctcca tggcgacttg gcatcgcttt
     7441 ctgcccgcct ctgccctcgc cctgcctgcc ccacccccca gcggagagca tgatccgcgc
     7501 ccttgcttct gactctagcc tctgggacac atcagtcaac gtgggcagtt cagtctgggt
     7561 tcgttccttt ctctgttctc atctggcccc ccagggcgat cttgttgctt tttgctggga
     7621 cctgcccagt ctagagaggg cctatttccc atgattcctt catatttgca tatacgatac
     7681 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     7741 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     7801 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     7861 atcttgtgga aaggacgaaa caccggccag atctgagcct gggagctctc tggccttatt
     7921 gatacatcat ctctgtatta ggctcccaac ttttttgaga cgtctagaat tcgatatcaa
     7981 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     8041 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     8101 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8161 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8221 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8281 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8341 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     8401 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8461 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8521 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     8581 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     8641 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     8701 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     8821 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     8881 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8941 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     9001 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     9061 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     9121 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9181 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9241 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     9301 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9361 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9421 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     9481 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     9541 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9601 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9661 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9721 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9781 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9841 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9961 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
    10021 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10261 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    10321 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    10381 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
    10441 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
    10501 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
    10561 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
    10621 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
    10681 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
    10741 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
    10801 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
    10861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
    10981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    11101 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    11161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    11281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    11341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    11401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    12001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13261 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
