LOCUS       sequence-430703-       20866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression TadDE-NG in rice cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20866)
  AUTHORS   Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T,
            Qi Y, Zhang Y
  TITLE     High performance TadA-8e derived cytosine and dual base editors with
            undetectable off-target effects in plants.
  JOURNAL   Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
  PUBMED    38877035
REFERENCE   2  (bases 1 to 20866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-14"; volume: "15(1):5103. doi"; pages: " 
            10.1038/s41467-024-49473-w"
COMMENT     Sequence Label: pGEL856
FEATURES             Location/Qualifiers
     source          1..20866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        173..219
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             294..1001
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     707..726
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1023..2048
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             1023..2048
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2089..2263
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2341..2365
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2790..3584
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5009..5597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5498..5517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5751..5768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5783..5923
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5849..5868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6009..6031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6267..6461
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6527..7600)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8029..8658)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9959..9983
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10186..10202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10778..10799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10814..10819,10820..10837,10838..10844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10852..10868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10876..10892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10888..11295
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     10911..10931
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10913..10931
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10978..10997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10979..10997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11007..11023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(12945..13193)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(13209..13229)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13272..13520)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(13551..13799)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(13836..13883)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             13963..13971
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(18009..18029)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(18630..18650)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(18696..20693)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(20740..20756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    20764..20780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(20788..20794,20795..20812,20813..20818))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20833..20854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
      181 cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc
      241 tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga gatatggtga
      301 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      361 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      421 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      481 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      541 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      601 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      661 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      721 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      781 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      841 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      901 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      961 agggccgcca ctccaccggc ggcatggacg agctttacaa gtcgggcgga ggtggctctg
     1021 atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     1081 acagcgtgtc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     1141 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     1201 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     1261 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     1321 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     1381 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     1441 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     1501 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     1561 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     1621 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     1681 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     1741 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     1801 cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     1861 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     1921 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     1981 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     2041 agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt tctccataat
     2101 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
     2161 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     2221 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc
     2281 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     2341 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     2401 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     2461 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     2521 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     2581 cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta
     2641 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
     2701 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
     2761 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
     2821 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
     2881 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
     2941 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
     3001 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
     3061 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
     3121 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
     3181 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
     3241 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
     3301 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     3361 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     3421 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     3481 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     3541 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga
     3601 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgct gatgaatccc ctaatgattt
     3661 ttatcaaaat cattaagtta aggtagatac acatcttgtc atatgatcaa atggtttcgc
     3721 caaaaatcaa taatcagaca acaaaatgtg cgaactcgat attttacacg actctcttta
     3781 ccaattctgc cccgaattac acttaaaacg actcaacagc ttaacgttgg cttgccacgc
     3841 cttacttgac tgtaaaactc tcactcttac cgaacttggc cgtaacctgc caaccaaagc
     3901 gagaacaaaa cataacatca aacgaatcga ccgattgtta ggtaatcgtc acctccacaa
     3961 agagcgactc gctgtatacc gttggcatgc tagctttatc tgttcgggca atacgatgcc
     4021 cattgtactt gttgactggt ctgatatccg tgagcaaaaa cggcttatgg tattgcgagc
     4081 ttcagtcgca ctacacggtc gttctgttac tctttatgag aaagcgttcc cgctttcaga
     4141 gcaatgttca aagaaagctc atgaccaatt tctagccgac cttgcgagca ttctaccgag
     4201 taacaccaca ccgctcattg tcagtgatgc tggctttaaa gtgccatggt ataaatccgt
     4261 tgagaagctg ggttggtact ggttaagtcg agtaagagga aaagtacaat atgcagacct
     4321 aggagcggaa aactggaaac ctatcagcaa cttacatgat atgtcatcta gtcactcaaa
     4381 gactttaggc tataagaggc tgactaaaag caatccaatc tcatgccaaa ttctattgta
     4441 taaatctcgc tctaaaggcc gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc
     4501 gtcacctaaa atctactcag cgtcggcaaa ggagccatgg attctagcaa ctaacttacc
     4561 tgttgaaatt cgaacaccca aacaacttgt taatatctat tcgaagcgaa tgcagattga
     4621 agaaaccttc cgagacttga aaagtcctgc ctacggacta ggcctacgcc atagccgaac
     4681 gagcagctca gagcgttttg atatcatgct gctaatcgcc ctgatgcttc aactaacatg
     4741 ttggcttgcg ggcgttcatg ctcagaaaca aggttgggac aagcacttcc aggctaacac
     4801 agtcagaaat cgaaacgtac tctcaacagt tcgcttaggc atggaagttt tgcggcattc
     4861 tggctacaca ataacaaggg aagacttact cgtggctgca accctactag ctcaaaattt
     4921 attcacacat ggttacgctt tggggaaatt atgaggggat ctctcagcac tgagcgtcag
     4981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     5161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5641 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5701 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5761 tgagcgagga agcggaagag ggcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5821 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5881 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     5941 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6001 gaccgtttcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     6061 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     6121 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     6181 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     6241 cttgggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     6301 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     6361 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     6421 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagagg ccttttcgac
     6481 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     6541 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     6601 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     6661 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     6721 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
     6781 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
     6841 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
     6901 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
     6961 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
     7021 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
     7081 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
     7141 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
     7201 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
     7261 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
     7321 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
     7381 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
     7441 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
     7501 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
     7561 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
     7621 ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc
     7681 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     7741 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     7801 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     7861 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     7921 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     7981 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     8041 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     8101 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     8161 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     8221 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     8281 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     8341 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     8401 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
     8461 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
     8521 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
     8581 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
     8641 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
     8701 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
     8761 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
     8821 aaaccggcca ggatttcatg cagccgcacg gttgagccgt gcgcgggcgg ctcgaacacg
     8881 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
     8941 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
     9001 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
     9061 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
     9121 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
     9181 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
     9241 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
     9301 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
     9361 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
     9421 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
     9481 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
     9541 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
     9601 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
     9661 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
     9721 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
     9781 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
     9841 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
     9901 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
     9961 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa
    10021 tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
    10081 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10141 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10201 gtgccacact gagctcgata tcggcgcgcc ccgggagctc agcggcaaaa cacacctaga
    10261 ctagatttgt tttgctaacc caattgatat taattatata tgattaatat ttatatgtat
    10321 atggaattgg ttaataaaat gcatctggtt catcaaagaa ttataaagac acgtgacatt
    10381 catttaggat aagaaatatg gatgatctct ttctcttatt cagataatta gtaattacac
    10441 ataacacaca actttgatgc ccacattata gtgattagca tgtcactatg tgtgcatcct
    10501 tttatttcat acattaatta acttggccaa tccagaagat ggacaagtct aggctcgagt
    10561 acctgcacca gccgggaatc gaacccgggt ctgtaccgtg gcagggtact attctaccac
    10621 tagaccactg gtgctttgtt gcaccgactc ggtgccactt tgctgtttcc agcaaagttg
    10681 ataacggact agccttattt aaacttgcta tgctgtttcc agcatagctc ttaaactgag
    10741 accaccggtg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10801 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10861 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10921 ctcactaaag ggaacaaaag ctggaggacc tcgagggggg gcccggtacc caattcgccc
    10981 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    11041 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11101 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11161 tgggacgcgc cctgtagcgg cgcccgtctc caggggggca cccgtcagtg cattgcgcga
    11221 tgcatgcact gcctccattg cggtcacgcc gccacgtttt gcaatcttca caagttggcc
    11281 tgtgtccaac tgtaaggtct cctgcaccag ccgggaatcg aacccgggtc tgtaccgtgg
    11341 cagggtacta ttctaccact agaccactgg tgctttgttc ctgcaggctg cacacacaat
    11401 taccgaagca tggtcagcaa ctatgaacat gaaattataa caataccaag caagtacagc
    11461 agaaatcagt caatcaaatt attccaatat gttagataca cgatctaaac cacaataaca
    11521 tcagtgtacc tatcaacacc aacagtgacc taacaaaata gagagtcgca ccgtgctcga
    11581 gttcacaaaa ataatctgca ggtctatgaa ctactagaat tccatcggca aactgattca
    11641 ataactacta gcactatccc gagagcacat aaggcaggtt aattaacata ggctagaatt
    11701 tacaggtgca acagttgata agataggcat aagcaagtaa aatatcctga aagctatgct
    11761 atgtaatcac taatcaacaa gacgcagccc ttagattaaa catgaacaga gtagaatcta
    11821 tgcaactaga gggactgaat caaaaacaac ctaagcaaaa tatacctgaa gctgctactt
    11881 ggatcaacga gaaaaacaga gactaaatcc tactgattga acagatcgga acaaatcaca
    11941 accctagcac agttctactt ctagatccga accaataaat acttggacca cgaacagcat
    12001 cgctcataat cacagatcca gcataccacg cgaaccccat ctaaccctgt tcaaatctac
    12061 cacagatcgc aaaacggaga ctaacaacaa catctcacaa tcacgaacag gccgggaaat
    12121 caaatcaaac gcgaggaacg gagaggatca gaaaatcgca taccttgaga aggcgattcg
    12181 acgcttcgct tcgaggggag attttttttt ctctgcgatt ttggggattc gattgatgga
    12241 ggaagaggcg aggagggggt ggggtattta taggtgggga gggggggact cgtgagtggg
    12301 cccaggggtg cggtgggtgg ggcccagtga gaatatagac aaataaagag agggagaggc
    12361 ggtttgggga gtccgtcccc accacgcaaa cgcgagaagg aaagaaagga acggaaaggg
    12421 ggcgggtcca gacggcgccg cggagatatc gaggaggtgg aaagctgatg ccgtttgctc
    12481 acggcggggt tggtggccgt taacacgcag cgaagcggcg ttactgccgt gtacgttttg
    12541 tgacctttcg aaagagagcc ccagcccaag aaaggctggc caggtttttt tgtactgtgt
    12601 acgattacga cggaagaaaa catactcgtt gttttgtatt ggtagatact gggccgtagt
    12661 aaaattagaa tcagttgtta attggttatt atatatacca tcttaagcta ccaaaattaa
    12721 taaagttgtg atggctatgg tatattttgg tagtgaatta tattgatttg gtatatatta
    12781 gaatcaatcc aaccacaaca caaccaaaaa tacctgaagc tcaatattga tggtgctttt
    12841 cgactatcta gcaaatgtgg tgaatggggc tttataattg aagcagccca ggatgcactt
    12901 tgtgcagaga ctgatgtgct ccggcgcgcc gtccgagctc agcgcttatc tttaatcata
    12961 ttccatagtc cataccatag cacatacagt agttatatgc tgcagaagag atccaacaaa
    13021 acattcacaa tggattatag aaacatttgt ttattcatta taatgtgatc ttacattcat
    13081 ttaatattag aaaaagccac aaattcataa cacaacaagc caagaaaaaa acacaaactt
    13141 aagcacacaa gctttttatt tgacacacca aatatttcat cttcatcttc atatactcga
    13201 gaagctcaca cttttctttt ctttttgggc tcaaactctg aaccatcagc agtacgcttt
    13261 gaacctcccg acagcatctt gattttattc tcgccgtttg aatcttgtat tactaacgcc
    13321 catggcttgt attctggagc atcggaggtc aataacatca cattctcgtc ggtagactcg
    13381 tcataagcag tatgcactag aatgtccgat tcgggcttgt tgccgatcac ctcctccact
    13441 tcttcaggga gcatcaggat gctctcttgg atgaccaact gcttcccagt ctctttctca
    13501 ataatatcac tcaggttcgt cgaaccgcct gacccccccg acccgccgga tagcattttg
    13561 attttgttct caccgttaga gtcctggatg accaacgccc acggcttgta ctcaggtgca
    13621 tcagaagtca aaagcatgac attctcatcc gtgctttcgt cgtacgcggt atgaacaaga
    13681 atgtcagatt caggcttgtt gccgatgacc tcctccacct cctccggaag catgagaatg
    13741 ctctcttgta taaccagctg tttcccagtt tccttctcga ttatatctga gagattagtg
    13801 cttcctccgg agccgccaga gccaccagat cctgccttct tcttcttcgc ctgccccgcc
    13861 ttcttcgtcg ccgctggccg cttgtcgccc ccgagctgag acaggtcgat gcgcgtctcg
    13921 tagaggccgg taatcgactg gtggatgagg gtcgcgtcca ggacctcctt agtgctgcgg
    13981 tagaccttgc gatcgatagt tgtgtcgaag tacttgaaag cgcgaggggc gccgaggttc
    14041 gtcagggtga agagatgaat gatattctca gcctgctccc tgattggctt gtcgcggtgc
    14101 ttgttgtacg cggagaggac cttatccaga ttcgcgtcgg ccaggatcac gcgcttggag
    14161 aactcggaaa tctgctcaat gatctcgtcg aggtaatgct tgtgctgctc gacgaacagc
    14221 tgcttctgct cgttgtcctc ggggctgccc ttgagcttct cgtagtggga ggccaggtag
    14281 aggaagttca catacttgga cggcagagcc agctcgttcc ccttctgcag gaagcgagcg
    14341 gaagccagca tccgcttcct gccgttctcc agctcgaaga gtgagtactt ggggagctta
    14401 atgatcaggt ccttcttcac ctccttgtag cccttcgcct ccaggaaatc gatcgggttc
    14461 ttctcgaagc tggagcgctc cataatcgtg atccccagca gctccttcac gctcttgagc
    14521 ttcttggact tgcccttctc aaccttcgcc acaaccagga ccgagtaggc cacagtgggg
    14581 ctaacgaacc cgccgtactt cttcggatcc cagtccttct tgcgggcgat gagcttgtcg
    14641 ctgttccgct tagggcgaat tgactcctta gagaacccgc cagtctggac ctctgtcttc
    14701 ttgacgatat tcacttgtgg catggagaga accttcctga cggtcgcgaa atccctgccc
    14761 ttgtcccaca cgatctcccc cgtctcgccg ttcgtctcga tgagggggcg cttccggatc
    14821 tcgccattgg ccagagtgat ctctgtcttg aagaaattca taatgttaga gtagaagaag
    14881 tacttggcgg tagccttgcc aatctcctgc tccgacttgg cgatcatctt cctcacatcg
    14941 taaaccttgt agtccccgta cacgaactcg ctctcgagct ttgggtactt cttgatcaga
    15001 gctgtgccga ccaccgcgtt caggtacgcg tcatgggcat ggtggtaatt gttgatctcc
    15061 cgaaccttgt agaactggaa atccttcctg aagtcggaga cgagctttga cttcagggtg
    15121 atgaccttca cctcgcggat cagcttgtca ttctcatcgt acttagtgtt catccgtgag
    15181 tcgagaatct gcgcaacgtg cttagtgatc tgccgtgtct cgaccagctg cctcttgatg
    15241 aagcccgcct tgtccagctc agagagcccg cccctctcag cctttgtgag gttatcgaac
    15301 ttccgctgcg tgatcagctt ggcattcagg agctggcgcc agtagttctt catcttctta
    15361 acgacctcct ctgaaggaac attatcagac ttgccccggt tcttgtccga cctggtgagg
    15421 accttgttgt caatggagtc atccttcagg aatgactgtg gaacgatatg atcgacgtcg
    15481 taatcgctga gcctgttaat atccagctcc tggtccacat acatatcgcg gccattctgg
    15541 aggtagtaca ggtagagctt ctcattctgc agctgcgtgt tctccaccgg gtgctccttg
    15601 aggatctggg accccagctc cttaatgccc tcctcgatcc tcttcatcct ctcgcgtgag
    15661 ttcttctggc ccttctgcgt ggtctgattc tcccgggcca tctcaatgac gatgttctca
    15721 ggcttgtgcc tgcccatgac cttcaccagc tcgtccacaa ccttcacggt ctgcagaatc
    15781 cccttcttga tagctggcga gccagcgagg ttcgcgatat gctcgtgcag cgagtccccc
    15841 tggccgctca cctgagcctt ctggatatcc tccttgaatg tgaggctgtc atcgtgaatc
    15901 agctgcatga aattgcggtt cgcgaagcca tcgctcttca ggaagtcgag gatcgtcttc
    15961 ccggactgct tgtcccgaat gccgttgatg agcttcctgc tcagcctccc ccagccggtg
    16021 tacctcctcc tcttgagctg cttcatgacc ttgtcatcga agagatgggc gtaagtcttc
    16081 aggcgctcct cgatcatctc ccggtcctcg aacagagtga gtgtcagcac aatgtcctcg
    16141 aggatatcct cattctcctc gttgtccagg aagtccttat ccttaatgat cttcaggaga
    16201 tcgtggtagg tccccaggga ggcgttgaag cggtcctcaa cgccagagat ctcgaccgaa
    16261 tcgaagcact caatcttctt gaagtagtcc tccttgagct gcttaaccgt gaccttccgg
    16321 ttggtcttga acaggaggtc cacgatggcc ttcttctgct ccccagacag gaaagccggc
    16381 ttcctcatgc cctcggtcac atacttcacc ttagtcagct cgttgtagac tgtgaagtac
    16441 tcgtacagga gcgagtgctt agggagcacc ttctcatttg gcaggttctt gtcgaaattc
    16501 gtcatcctct cgatgaacga ctgagcgcta gcgcccttgt cgaccacctc ctcgaagttc
    16561 cacggcgtga tcgtctcctc tgacttgcgg gtcatccaag cgaagcggga gttgccccta
    16621 gcgagtgggc cgacgtagta cgggatcctg aaagtcagaa tcttctcgat cttctcgcgg
    16681 ttatccttga ggaaagggta gaagtcctcc tgcctcctca ggatagcgtg cagctccccg
    16741 agatgaatct ggtgtgggat gctgccgtta tcgaatgtcc gctgcttcct caggaggtcc
    16801 tcgcgattga gcttcaccag cagctcctcc gtgccgtcca tcttctccag aatcggcttg
    16861 atgaacttgt agaactcctc ctgagaggcc ccgccgtcaa tgtacccagc gtagccgttc
    16921 ttcgactgat cgaagaagat ctccttgtac ttctcgggga gctgctgcct gaccagcgcc
    16981 ttcaggaggg tcagatcctg atggtgctcg tcgtagcgct tgatcatgga ggctgagagc
    17041 ggagccttcg taatctcggt gttcaccctg agaatatcag acaggaggat ggcgtccgac
    17101 agattcttgg cagcgaggaa caggtccgcg tactgatcgc cgatctgggc caggaggtta
    17161 tccaggtcat cgtcgtatgt gtccttggag agctgcagct tggcgtcctc agcgagatcg
    17221 aaattcgact tgaagttggg cgtgagcccc aggctgagcg caatgagatt cccgaacagg
    17281 ccgttcttct tctcgcccgg cagctgggcg atcaggttct cgaggcgccg agacttcgag
    17341 agcctagcgg acaggatagc cttcgcgtcg acgcctgacg cattaatggg gttctcctcg
    17401 aagagctggt tgtacgtctg cacgagctgg atgaacagct tgtcaacatc gctattgtcc
    17461 gggttgagat ccccctcgat caggaaatgg cccctgaact taatcatgtg ggccagagcg
    17521 aggtagatca ggcggaggtc cgccttatct gtggagtcca cgagcttctt ccgcagatgg
    17581 tagatcgtag ggtacttctc gtggtaggca acctcgtcga caatgttgcc gaagattgga
    17641 tgccgctcgt gcttcttatc ctcctccacg aggaatgact cctccagcct gtggaagaaa
    17701 gaatcgtcaa ccttcgccat ctcgttggag aaaatctcct gcaggtagca gatgcgattc
    17761 ttcctgcgcg tgtaccgcct gcgggcggtg cgcttgagcc gcgtagcctc agccgtctcg
    17821 ccgctgtcga acaggagagc gccaatgaga ttcttcttga tggaatgccg atcggtgttg
    17881 cccaggacct tgaacttctt tgagggcacc ttgtactcgt cggtgatcac ggcccagcca
    17941 acagagttag tcccaatagc gaggccgatc gagtacttct tgtcagcagc tggcaccccg
    18001 tggatgccaa ccttcctctt cttcttcgga gccatcgaac ccccgctgct gcccccgctg
    18061 ctttcaggtg ttgcgctctc gcttgtgcca ggtgtctcag agccagagga gcctccgcta
    18121 gatcctccgg agttgatgga gctctgggcc ttcttctgag cattgaacac ctgtctaggc
    18181 atccgataga aatcgcacag cagggcggca cattcatctg ccaggattcc ctcggtaatt
    18241 tcgacgcggt gattcatgcc ggggtagttc agcacgttca tcagggagcc tgcggcgcct
    18301 ctttttgagt tcctcacgcc aaacaccacg cggccgatcc tagagttgat catggcgccg
    18361 gcgcacatca cgcaaggctc gaatgtcacg tacagggtgg cgtcaatcag tcgtgagttc
    18421 tgcatgacca ggccgccctg tctcagggcc ataatttcgg catgggctgt tgggtcgtgc
    18481 aggccgatgc ggcggttcca gccctcgccg atcactctat tgttcagcac cagcacggct
    18541 cccacaggcg cctccccctc atcccgtgcc ctcttggcca gggtcagggc atgtctcatc
    18601 cagtactcgt gggaaaactc cacctcagag actttccgct tcttctttgg tgactcgaac
    18661 tcgcttccgt cggctgtccg tttcattcct gcaggctgca gaagtaacac caaacaacag
    18721 ggtgagcatc gacaaaagaa acagtaccaa gcaaataaat agcgtatgaa ggcagggcta
    18781 aaaaaatcca catatagctg ctgcatatgc catcatccaa gtatatcaag atcaaaataa
    18841 ttataaaaca tacttgttta ttataataga taggtactca aggttagagc atatgaatag
    18901 atgctgcata tgccatcatg tatatgcatc agtaaaaccc acatcaacat gtatacctat
    18961 cctagatcga tatttccatc catcttaaac tcgtaactat gaagatgtat gacacacaca
    19021 tacagttcca aaattaataa atacaccagg tagtttgaaa cagtattcta ctccgatctt
    19081 gaacgaatga acgaccgccc aaccacacca catcatcaca accaagcgaa caaaaagcat
    19141 ctctgtatat gcatcagtaa aacccgcatc aacatgtata cctatcctag atcgatattt
    19201 ccatccatca tcttcaattc gtaactatga atatgtatgg cacacacata cagatccaaa
    19261 attaataaat ccaccaggta gtttgaaaca gaattaattc tactccgatc ttgaacgacc
    19321 gcccaaccag accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac
    19381 aaacaagtgc acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg
    19441 aaacaaaaaa aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt
    19501 ccccaaagag aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc
    19561 cgtgtacgaa cgctagtagc acggatctaa cacaaacacg gatctaacac aaacatgaac
    19621 agaagtagaa ctaccgggcc ctaaccatgg accggaacgc cgatcttgag aaggtagaga
    19681 gggggggggg ggaggacgag cggcgtacct tgaagcggag gtgccgacgg gtggatttgg
    19741 gggagttctg gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga aagagggtgt
    19801 ggagggggtg tctatttatt acggcgggcg aggaagggaa agcgaaggag cggtgggaaa
    19861 ggaatccccc gtagctgccg gtgccgtgag aggaggagga ggccgcctgc cgtgccggct
    19921 cacgtctgcc gctccgccac gcaatttctg gatgccgaca gcggagcaag tccaacggtg
    19981 gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc gtctgcttcg
    20041 cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc gtcgacggcg
    20101 tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt ttttttaatt
    20161 tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt atatctaaat
    20221 tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc taaaatagaa
    20281 taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa ccctaaatgg
    20341 atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa aaaaaaggag
    20401 aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc
    20461 ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat attattgtag
    20521 tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag atagataaac
    20581 tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac ttagacatgc
    20641 aatgctcacc ttatctcaag agaggggcac gaccgggtca cgctgcactg cagctccggc
    20701 gcgccgatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
    20761 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    20821 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgc
//
