LOCUS       sequence-430709-       12840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes sgRNA that target EGFP with stretch sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Ui-Tei K, Maruyama S, Nakano Y
  TITLE     Enhancement of single guide RNA transcription for efficient 
            CRISPR/Cas-based genomic engineering.
  JOURNAL   Genome. 2017 Jun;60(6):537-545. doi: 10.1139/gen-2016-0127. Epub 
            2017 Jan 26.
  PUBMED    28177825
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1139/gen-2016-0127"; journalName: "Genome"; date: "2017-06"; 
            volume: "60"; issue: "6"; pages: "537-545"
COMMENT     Sequence Label: pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)
FEATURES             Location/Qualifiers
     source          1..12840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    566..907
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             974..1390
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1409..1425
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(1440..1515)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1542..1756)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        1767..2266
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    3043..3384
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3451..3867
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3886..3902
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(3917..3992)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4019..4233)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        4244..4743
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4768..5367
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     protein_bind    5953..5969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5977..5983,5984..6001,6002..6007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6022..6043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6331..6919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7090..7881,7882..7950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7951..8055)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9896..9912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9920..9926,9927..9944,9945..9950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9965..9986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10274..10862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11033..11824,11825..11893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11894..11998)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(12826..12840,1..502)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
       61 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
      121 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
      181 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
      241 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
      301 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
      361 ccagtcctcc gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg
      421 tttgcatccg aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg
      481 cccacctcgg gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg
      541 accaccgacc cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt
      601 ctttgtgcgt gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttagctaa
      661 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
      721 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
      781 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
      841 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
      901 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
      961 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1021 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1081 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1141 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1201 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1261 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1321 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1381 tctaggcgcc ggaattagat cgatctctcg aggtcgacgg tatcgataag cttaaaaaag
     1441 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
     1501 ttctagctct aaaaccggtg aacagctcct cgcccggatc tgtggtctca tacagaactt
     1561 ataagattcc caaatccaaa gacatttcac gtttatggtg atttcccaga acacatagcg
     1621 acatgcaaat attgcagggc gccactcccc tgtccctcac agccatcttc ctgccagggc
     1681 gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc gcgattcctt ggagcgggtt
     1741 gatgacgtca gcgttcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     1801 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     1861 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     1921 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     1981 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     2041 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     2101 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     2161 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     2221 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     2281 ctagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     2341 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     2401 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     2461 cgggtgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     2521 gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga acagagagac
     2581 agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     2641 aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat
     2701 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     2761 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     2821 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtattccc
     2881 aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg ggagggtctc
     2941 ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca ccgagatttg
     3001 gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc cagcggtcgt
     3061 ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt
     3121 ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc
     3181 tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg tttttgtggc
     3241 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
     3301 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
     3361 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
     3421 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
     3481 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
     3541 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
     3601 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
     3661 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
     3721 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
     3781 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
     3841 ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg tcgacggtat
     3901 cgataagctt aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
     3961 ttattttaac ttgctatttc tagctctaaa accggtgaac agctcctcgc ccggatctgt
     4021 ggtctcatac agaacttata agattcccaa atccaaagac atttcacgtt tatggtgatt
     4081 tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt ccctcacagc
     4141 catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac tgggcccgcg
     4201 attccttgga gcgggttgat gacgtcagcg ttcgaattct accgggtagg ggaggcgctt
     4261 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4321 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4381 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4441 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4501 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4561 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4621 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     4681 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     4741 tcgacctgca gcccaagcta gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4801 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4861 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4921 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4981 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5041 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5101 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5161 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5221 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5281 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5341 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     5401 aggagcgcac gaccccatgc atcgataaaa taaaagattt tatttagtct ccagaaaaag
     5461 gggggaatga aagaccccac ctgtaggttt ggcaagctag agaaccatca gatgtttcca
     5521 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     5581 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     5641 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac
     5701 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     5761 gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca
     5821 acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga
     5881 atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg
     5941 agaattgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6001 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6061 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8041 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8101 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     8161 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     8221 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     8281 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8341 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     8401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     8461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     8521 acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     8581 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     8641 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     8701 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt
     8761 aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
     8821 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg
     8881 ggaactcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     8941 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9001 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9061 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9121 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9181 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9241 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9301 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     9361 tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     9421 cacctgtagg tttggcaagc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     9481 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     9541 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     9601 tccgatagac tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     9661 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     9721 cgggggtctt tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga
     9781 gtcgaatatt aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata
     9841 ctcctgaaat agttcattat ggacagcgca gaaagagctg gggagaattg tgaaattgtt
     9901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12001 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    12061 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    12121 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    12181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    12241 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    12301 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    12361 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    12421 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    12481 cggcgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
    12541 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
    12601 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
    12661 ggccacgatg cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt
    12721 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
    12781 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
//
