LOCUS       sequence-43080-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Mattingly RR, Macara IG
  TITLE     Phosphorylation-dependent activation of the Ras-GRF/CDC25Mm exchange
            factor by muscarinic receptors and G-protein beta gamma subunits.
  JOURNAL   Nature. 1996 Jul 18. 382(6588):268-72.
  PUBMED    8717044
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1996 Jul 18. 382(6588):268-72."
COMMENT     Sequence Label: pKH3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        70..373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        374..577
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     528..548
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     574..598
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        808..826
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     808..825
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     839..858
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
       61 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      121 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      181 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      241 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      301 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      361 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      421 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      481 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      541 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
      601 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      661 ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac
      721 cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata
      781 cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt
      841 gcctttctct ccacaggtgt ccactcccag gtcc
//
