LOCUS       sequence-43085-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ351 - MCS(cxTi10882, IV)
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(190..214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagggtac cagagctcac ctaggtatct agaaccggtg acgtcaccat gggaagcttc
       61 gtggatccag atatcctgca ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa
      121 gatctgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
      181 ctgcccgtga cccagctttc ttgtacaaag tggatagaat caagcatgct ccgcgtttgc
      241 tgtactattt ctcgcagagc agtttttttt ctcacacttt attattctgt actgcccctc
      301 cacgctataa ttttttgcat agagggccat aaaggaaaac ggtacggtaa aaataatggg
      361 tcctttttac cgttgtgtag attgtttcta acgggatgca aattttcact aaaactctgg
      421 aaaactgatg gaattctacg ggcagccggc aattttagat atttgaagaa tccttcttga
      481 tcagtgccaa gctttcccat ggaccaaaga tgtggatcca agatcaaatg cacaggatct
      541 gaactccttt gttccaaaaa caaggatttc ttttctatag tttgacctaa atattatagt
      601 agaacatata catttgatga tttcatgaat aatttattgt taaaaaataa tacatgt
//
