LOCUS       sequence-430920-       11183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses bicone gas vesicles in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Ling B, Gungoren B, Yao Y, Dutka P, Vassallo R, Nayak R, Smith CAB, 
            Lee J, Swift MB, Shapiro MG
  TITLE     Truly Tiny Acoustic Biomolecules for Ultrasound Imaging and Therapy.
  JOURNAL   Adv Mater. 2024 Feb 26:e2307106. doi: 10.1002/adma.202307106.
  PUBMED    38409678
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Mater. 
            2024 Feb 26:e2307106. doi: 10.1002/adma.202307106."
COMMENT     Sequence Label: A2C-∆N
FEATURES             Location/Qualifiers
     source          1..11183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2016..3098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3060..3079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3099..3176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3382..3401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3485..3504
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3485..3503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3504..3528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5868..5885
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(9602..9620)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9616..9663
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9700..10155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9787..9806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9997..10018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(10247..11062)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10247..11062)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10970..10989
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11054..11073
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattccaa gcttcgccag
     3541 ggttttccca gtcgagctca tgactcatat ttatagttat tctcctcatt tcccaagttt
     3601 ccgacaacaa ataacaagga attaaaaaaa tggcagttga aaaaaccaat tcttcctcca
     3661 gcttggcaga agttattgat agaatcctcg acaaaggtat cgtaattgac gcttgggttc
     3721 gtgtttcctt agttggtatc gaactactag caattgaagc tcggatcgtt atcgcttccg
     3781 ttgaaaccta cttgaagtat gctgaagcag ttggtttgac ccaatcagca gcagtacctg
     3841 cttaatttaa ttaagcaagt taccaagtag gagaataacc atgactcata tttatagtta
     3901 ttctcctcat ttcccaagtt tccgacaaca aataacaagg aattaaaaaa atggcagttg
     3961 aaaaaaccaa ttcttcctcc agcttggcag aagttattga tagaatcctc gacaaaggta
     4021 tcgtaattga cgcttgggtt cgtgtttcct tagttggtat cgaactacta gcaattgaag
     4081 ctcggatcgt tatcgcttcc gttgaaacct acttgaagta tgctgaagca gttggtttga
     4141 cccaatcagc agcagtacct gcttaattta attaagctcc ataatatcag gattaattaa
     4201 tcctgatatt ggttctgctt tttttaatta tctagatatt tggaaatcaa cgcttctcaa
     4261 aggatattac aaaaaagtac gaatccttat aatgtagtac ctggagaagc aatttctgtt
     4321 aggtctggga gctcccatgg gatcgttatc ccgcggccaa gcttgctagc gctaatttac
     4381 tattttttgg agaacttcat gatttcttta atggcaaaaa tccggcaaga acatcagtca
     4441 atagcagaga aagtggctga actatctctt gagaccagag aattcttgtc cgtcacgaca
     4501 gcgaaaagac aagagcaagc tgaaaaacaa gctcaagaac tgcaagcatt ctacaaggat
     4561 cttcaggaaa caagtcagca gtttttatca gaaacagccc aagccagaat tgctcaagct
     4621 gaaaaacaag ctcaagaact gttagcattc cacaaagaac ttcaagaaac aagtcagcag
     4681 tttttatcag caacagccca agccagaatt gctcaagctg aaaaacaagc gcaagaactg
     4741 ttagcatttt atcaagaagt tcgggaaaca agtcagcagt ttttatcagc aacagcccaa
     4801 gcaagaattg ctcaagctga aaaacaagct caagaactgt tagcattcca caaagaactt
     4861 caagaaacaa gtcagcagtt tttatcagca acagccgacg caagaactgc tcaagctaag
     4921 gaacagaagg aatctctcct gaaattccgt caggatttgt ttgtgagtat ctttggttaa
     4981 taaagatcta actattggag gctactaaaa atggaaatta aaaaaattat gcaagccgtg
     5041 aacgactttt tcggtgaaca cgtagctcct cctcataaaa ttacctcggt ggaagctact
     5101 gaagatgaag gttggagagt tattgttgaa gtcattgaag aacgagaata tatgaaaaaa
     5161 tacgccaaag atgaaatgct cggaacgtac gagtgctttg taaataaaga aaaagaagtc
     5221 atttcattca aacgactcga cgtcagatat agaagcgcca ttggcattga agcataaacg
     5281 ataagatggc aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt
     5341 agcgccattc aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa
     5401 gctgaaacat ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg
     5461 aaaatttatc atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt
     5521 atggacaaga acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa
     5581 gacgtaaaag ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc
     5641 aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa
     5701 gtaaacgaaa atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca
     5761 gccggttatt atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg
     5821 caaaaagaag tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa
     5881 gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa
     5941 gatgaagcga agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc
     6001 gattttcatt acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta
     6061 gaagagaaat aacgtgcttc acaaattagt aaccgcaccc attaaccttg tagtgaaaat
     6121 cggcgaaaaa gtacaggaag aagctgataa acagctatat gaccttccga cgattcagca
     6181 aaagctcatt cagcttcaaa tgatgtttga gcttggtgaa attccagaag aagcgtttca
     6241 agaaaaagaa gatgaattgt taatgaggta cgaaattgcg aaacgcagag aaattgaaca
     6301 atgggaagag ctaacacaaa aaagaaatga ggaatcctag atgggagaat tactgtattt
     6361 atacggttta attccaacaa aagaagcagc agccatagag ccgtttccat cttataaggg
     6421 gtttgacgga gaacattcac tgtacccaat tgcgtttgat caggtgacgg ctgtagtttc
     6481 taagctggat gctgacacct attcagaaaa agtgattcaa gaaaaaatgg agcaggatat
     6541 gagctggctg caggaaaaag catttcatca tcacgaaacg gtagccgctt tgtacgaaga
     6601 atttacgatc attccattaa aattttgcac catttataaa ggtgaagaaa gtctgcaggc
     6661 agctattgag attaacaaag aaaagataga gaattcactg acgctgcttc aaggaaatga
     6721 agagtggaat gtgaaaattt actgtgatga tacagagctt aaaaaaggaa tcagcgaaac
     6781 gaatgaaagc gtgaaagcga aaaaacaaga aattagtcac ttatcaccag gaagacagtt
     6841 ttttgaaaag aaaaaaatag atcagctgat tgaaaaagaa ttagagcttc acaaaaacaa
     6901 agtgtgtgaa gagatacatg acaagctaaa agaattatcg ctttatgact ctgttaaaaa
     6961 gaattggagc aaagacgtaa ctggcgcagc tgaacagatg gcgtggaaca gcgtgtttct
     7021 tctcccgtct ctgcagatta ctaagttcgt aaacgaaata gaagagcttc agcaaaggct
     7081 tgaaaataaa ggctggaagt ttgaagtgac gggaccatgg ccgccctatc atttctcgag
     7141 ctttgcgtaa agtgaggaat taacattatg tctcttaaac aatccatgga gaataaagat
     7201 attgctctta ttgatatttt agatgtcatt ttagataaag gagtcgccat taaaggagac
     7261 ttaatcattt ccatagctgg cgtcgattta gtgtatttgg atttgcgggt gcttatttct
     7321 tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca aaccaatcac ttctgaacaa
     7381 tttgataaac aaaaggagga attaatggat gcaaccggtc agccaagcaa atggacgaat
     7441 ccacttggat cctgatcaag ctgaacaagg cttagcgcag cttgtgatga cagttattga
     7501 gctattgagg caaatcgttg aacgtcatgc catgaggcgg gtggagggtg gaacgttgac
     7561 ggacgaacaa attgaaaact taggaattgc actaatgaac ttagaagaaa aaatggacga
     7621 gttgaaagag gtgttcggtc tggatgcaga agatttaaat attgatcttg gaccgctagg
     7681 cagcctgctt taagcggtca gtaggaggaa cagtatggca gtcgaacata atatgcagtc
     7741 aagtacgatt gtagatgtgc tcgaaaagat tttggataaa ggagtcgtta tagcggggga
     7801 catcaccgta ggaattgcag atgtcgagct attaacgata aagatccgct tgattgtggc
     7861 ttcggttgat aaggcaaaag aaatcggcat ggactggtgg gaaaatgatc cgtatctcag
     7921 ttcaaaagga gccaataaca aagcgctcga agaagaaaat aaaatgctgc atgagcggtt
     7981 aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa ctgtacgcta cttaaaaaat
     8041 ggagggattt acaatggcaa ctgaaacaaa attagataac acacaggcag aaaacaagga
     8101 aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt tcaaaagcaa gcaaaacaac
     8161 aagcagcggg ccaatcaaac gagcggtagc aggaggcatc atcggtgcaa cgattggata
     8221 tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc attgatacag acgaattaaa
     8281 aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa tcaaaaagca gcgtggccag
     8341 cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa gataaatcaa aagatgatga
     8401 agaaaacgta aattcttcta gtagcgaaac agaagacgat aacgttcaag agtacgacga
     8461 gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca cagcttgaag aaaaaatgaa
     8521 catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa gaagcggaag atacagattc
     8581 cgacgaagaa gagaacgatg agaacgatga aaacgatgaa aacgagcagg acgatgaaaa
     8641 cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa gaagctgagg aagaagaaag
     8701 tgaaagtgac gaagacagcg aggaagaaga ggaagattct cgctcaaaca aaaaaaataa
     8761 aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa gaagaaaaaa aggaagcgaa
     8821 accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa gcaaagaaaa acacggacga
     8881 agaagatgat gaagcaacat ctctttctag tgaagacgat acaacagcct aagacgtaaa
     8941 ggaggaaaga aagacatgag tacaggccct tctttttcaa ctaaagacaa tacgcttgaa
     9001 tactttgtga aagcttctaa taaacacggc ttttcacttg atatttcatt aaatgtaaac
     9061 ggcgctgtga tttccggtac catgatttca gcaaaagaat actttgatta cttaagcgaa
     9121 acgtttgaag aaggcagtga agtggctcag gcgctaagcg aacaattctc tttagcaagc
     9181 gaagcgagcg aatcaaacgg agaagcagaa gcccatttta ttcatttgaa aaatacaaag
     9241 atttactgtg gagacagtaa atctactcct tctaaaggca aaatcttttg gagagggaaa
     9301 atagcagaag tagacgggtt tttcttagga aagatttctg atgcaaaatc aacgagtaaa
     9361 aagagttcat aaaaaacggc ggggtgattg ccccgccgtt ttttagtgat gtgatgagat
     9421 gtgcagcttc ttttttcgat acatgagcgg agtcatcttc atttcttttc gaaactgatt
     9481 aataaaatag cttgtactat taaaccctac ttgataggcg acttccgtca catttgcttc
     9541 tgcttgctgc aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     9601 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     9661 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
     9721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9841 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9901 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9961 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    10021 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    10081 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    10141 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10201 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
    10261 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
    10321 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
    10381 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
    10441 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
    10501 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
    10561 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
    10621 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
    10681 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
    10741 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
    10801 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
    10861 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
    10921 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
    10981 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
    11041 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
    11101 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11161 gtagaaaaga tcaaaggatc ttc
//
