LOCUS       sequence-430951-       21266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression TadCBEa in rice cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T,
            Qi Y, Zhang Y
  TITLE     High performance TadA-8e derived cytosine and dual base editors with
            undetectable off-target effects in plants.
  JOURNAL   Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
  PUBMED    38877035
REFERENCE   2  (bases 1 to 21266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-14"; volume: "15(1):5103. doi"; pages: " 
            10.1038/s41467-024-49473-w"
COMMENT     Sequence Label: pGEL850
FEATURES             Location/Qualifiers
     source          1..21266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        178..523
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     466..486
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             596..1303
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1009..1028
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1325..2350
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             1325..2350
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2391..2565
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2643..2667
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3092..3886
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5311..5899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5800..5819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6053..6070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6085..6225
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6151..6170)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6311..6333
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6569..6763
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6829..7902)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8331..8960)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10261..10285
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10585..10601
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11177..11198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11213..11218,11219..11236,11237..11243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11251..11267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11275..11291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11287..11694
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     11310..11330
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11312..11330
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11377..11396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11378..11396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11406..11422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(13344..13592)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(13608..13628)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13671..13919)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(13950..14198)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(14235..14282)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(14283..18356,18357..18359,18360..18383))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14362..14370
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(18408..18428)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(19029..19049)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(19095..21092)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(21139..21155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    21163..21179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(21187..21193,21194..21211,21212..21217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    21232..21253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
       61 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
      121 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      181 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      241 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      301 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      361 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      421 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      481 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
      541 tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat gagatatggt
      601 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      661 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      721 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      781 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      841 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      901 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      961 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1021 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1081 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1141 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1201 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1261 cgagggccgc cactccaccg gcggcatgga cgagctttac aagtcgggcg gaggtggctc
     1321 tgatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     1381 cgacagcgtg tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     1441 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     1501 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     1561 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     1621 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     1681 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     1741 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     1801 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     1861 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     1921 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     1981 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     2041 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     2101 gccacgactc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     2161 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     2221 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     2281 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     2341 aaagaaatag agtagatgcc gaccgggatc tgtcgatcga caagctcgag tttctccata
     2401 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     2461 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     2521 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
     2581 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     2641 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     2701 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     2761 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     2821 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     2881 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
     2941 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     3001 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     3061 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     3121 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     3181 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     3241 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     3301 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     3361 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     3421 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     3481 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     3541 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     3601 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     3661 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     3721 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     3781 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     3841 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
     3901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg ctgatgaatc ccctaatgat
     3961 ttttatcaaa atcattaagt taaggtagat acacatcttg tcatatgatc aaatggtttc
     4021 gccaaaaatc aataatcaga caacaaaatg tgcgaactcg atattttaca cgactctctt
     4081 taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac
     4141 gccttacttg actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa
     4201 gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac
     4261 aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg
     4321 cccattgtac ttgttgactg gtctgatatc cgtgagcaaa aacggcttat ggtattgcga
     4381 gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca
     4441 gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg
     4501 agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc
     4561 gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac
     4621 ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca
     4681 aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca aattctattg
     4741 tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac
     4801 ccgtcaccta aaatctactc agcgtcggca aaggagccat ggattctagc aactaactta
     4861 cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt
     4921 gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga
     4981 acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca
     5041 tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac
     5101 acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat
     5161 tctggctaca caataacaag ggaagactta ctcgtggctg caaccctact agctcaaaat
     5221 ttattcacac atggttacgc tttggggaaa ttatgagggg atctctcagc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6061 agtgagcgag gaagcggaag agggcctgat gcggtatttt ctccttacgc atctgtgcgg
     6121 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6181 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6241 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6301 gtgaccgttt ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6361 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     6421 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     6481 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     6541 ctcttgggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     6601 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     6661 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     6721 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga ggccttttcg
     6781 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     6841 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     6901 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     6961 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7021 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7081 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7141 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7201 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7261 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     7321 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     7381 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     7441 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     7501 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     7561 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     7621 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     7681 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     7741 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     7801 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     7861 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     7921 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
     7981 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     8041 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     8101 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     8161 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     8221 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     8281 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     8341 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     8401 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     8461 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     8521 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     8581 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     8641 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     8701 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     8761 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     8821 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     8881 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     8941 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     9001 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     9061 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     9121 acaaaccggc caggatttca tgcagccgca cggttgagcc gtgcgcgggc ggctcgaaca
     9181 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     9241 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
     9301 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
     9361 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
     9421 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
     9481 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
     9541 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
     9601 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
     9661 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
     9721 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
     9781 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
     9841 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
     9901 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
     9961 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    10021 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    10081 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    10141 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    10201 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    10261 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    10321 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    10381 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10441 aaggcgatta agttgggtaa cgccagggyt ktyssrrwsr ssrmkykgtr mrrssmysky
    10501 crstrttacr ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10561 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccacactg agctcgatat
    10621 cggcgcgccc cgggagctca gcggcaaaac acacctagac tagatttgtt ttgctaaccc
    10681 aattgatatt aattatatat gattaatatt tatatgtata tggaattggt taataaaatg
    10741 catctggttc atcaaagaat tataaagaca cgtgacattc atttaggata agaaatatgg
    10801 atgatctctt tctcttattc agataattag taattacaca taacacacaa ctttgatgcc
    10861 cacattatag tgattagcat gtcactatgt gtgcatcctt ttatttcata cattaattaa
    10921 cttggccaat ccagaagatg gacaagtcta ggctcgagta cctgcaccag ccgggaatcg
    10981 aacccgggtc tgtaccgtgg cagggtacta ttctaccact agaccactgg tgctttgttg
    11041 caccgactcg gtgccacttt gctgtttcca gcaaagttga taacggacta gccttattta
    11101 aacttgctat gctgtttcca gcatagctct taaactgaga ccaccggtgg aaagcgggca
    11161 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11221 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11281 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    11341 tggaggacct cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg
    11401 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11461 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11521 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    11581 gcccgtctcc aggggggcac ccgtcagtgc attgcgcgat gcatgcactg cctccattgc
    11641 ggtcacgccg ccacgttttg caatcttcac aagttggcct gtgtccaact gtaaggtctc
    11701 ctgcaccagc cgggaatcga acccgggtct gtaccgtggc agggtactat tctaccacta
    11761 gaccactggt gctttgttcc tgcaggctgc acacacaatt accgaagcat ggtcagcaac
    11821 tatgaacatg aaattataac aataccaagc aagtacagca gaaatcagtc aatcaaatta
    11881 ttccaatatg ttagatacac gatctaaacc acaataacat cagtgtacct atcaacacca
    11941 acagtgacct aacaaaatag agagtcgcac cgtgctcgag ttcacaaaaa taatctgcag
    12001 gtctatgaac tactagaatt ccatcggcaa actgattcaa taactactag cactatcccg
    12061 agagcacata aggcaggtta attaacatag gctagaattt acaggtgcaa cagttgataa
    12121 gataggcata agcaagtaaa atatcctgaa agctatgcta tgtaatcact aatcaacaag
    12181 acgcagccct tagattaaac atgaacagag tagaatctat gcaactagag ggactgaatc
    12241 aaaaacaacc taagcaaaat atacctgaag ctgctacttg gatcaacgag aaaaacagag
    12301 actaaatcct actgattgaa cagatcggaa caaatcacaa ccctagcaca gttctacttc
    12361 tagatccgaa ccaataaata cttggaccac gaacagcatc gctcataatc acagatccag
    12421 cataccacgc gaaccccatc taaccctgtt caaatctacc acagatcgca aaacggagac
    12481 taacaacaac atctcacaat cacgaacagg ccgggaaatc aaatcaaacg cgaggaacgg
    12541 agaggatcag aaaatcgcat accttgagaa ggcgattcga cgcttcgctt cgaggggaga
    12601 tttttttttc tctgcgattt tggggattcg attgatggag gaagaggcga ggagggggtg
    12661 gggtatttat aggtggggag ggggggactc gtgagtgggc ccaggggtgc ggtgggtggg
    12721 gcccagtgag aatatagaca aataaagaga gggagaggcg gtttggggag tccgtcccca
    12781 ccacgcaaac gcgagaagga aagaaaggaa cggaaagggg gcgggtccag acggcgccgc
    12841 ggagatatcg aggaggtgga aagctgatgc cgtttgctca cggcggggtt ggtggccgtt
    12901 aacacgcagc gaagcggcgt tactgccgtg tacgttttgt gacctttcga aagagagccc
    12961 cagcccaaga aaggctggcc aggttttttt gtactgtgta cgattacgac ggaagaaaac
    13021 atactcgttg ttttgtattg gtagatactg ggccgtagta aaattagaat cagttgttaa
    13081 ttggttatta tatataccat cttaagctac caaaattaat aaagttgtga tggctatggt
    13141 atattttggt agtgaattat attgatttgg tatatattag aatcaatcca accacaacac
    13201 aaccaaaaat acctgaagct caatattgat ggtgcttttc gactatctag caaatgtggt
    13261 gaatggggct ttataattga agcagcccag gatgcacttt gtgcagagac tgatgtgctc
    13321 cggcgcgccg tccgagctca gcgcttatct ttaatcatat tccatagtcc ataccatagc
    13381 acatacagta gttatatgct gcagaagaga tccaacaaaa cattcacaat ggattataga
    13441 aacatttgtt tattcattat aatgtgatct tacattcatt taatattaga aaaagccaca
    13501 aattcataac acaacaagcc aagaaaaaaa cacaaactta agcacacaag ctttttattt
    13561 gacacaccaa atatttcatc ttcatcttca tatactcgag aagctcacac ttttcttttc
    13621 tttttgggct caaactctga accatcagca gtacgctttg aacctcccga cagcatcttg
    13681 attttattct cgccgtttga atcttgtatt actaacgccc atggcttgta ttctggagca
    13741 tcggaggtca ataacatcac attctcgtcg gtagactcgt cataagcagt atgcactaga
    13801 atgtccgatt cgggcttgtt gccgatcacc tcctccactt cttcagggag catcaggatg
    13861 ctctcttgga tgaccaactg cttcccagtc tctttctcaa taatatcact caggttcgtc
    13921 gaaccgcctg acccccccga cccgccggat agcattttga ttttgttctc accgttagag
    13981 tcctggatga ccaacgccca cggcttgtac tcaggtgcat cagaagtcaa aagcatgaca
    14041 ttctcatccg tgctttcgtc gtacgcggta tgaacaagaa tgtcagattc aggcttgttg
    14101 ccgatgacct cctccacctc ctccggaagc atgagaatgc tctcttgtat aaccagctgt
    14161 ttcccagttt ccttctcgat tatatctgag agattagtgc ttcctccgga gccgccagag
    14221 ccaccagatc ctgccttctt cttcttcgcc tgccccgcct tcttcgtcgc cgctggccgc
    14281 ttgtcgcccc cgagctgaga caggtcgatg cgcgtctcgt agaggccggt aatcgactgg
    14341 tggatgaggg tcgcgtccag gacctcctta gtgcttgtgt acctcttgcg atcgatagtt
    14401 gtgtcgaagt acttgaaagc agcaggggcg ccgaggttcg tcagggtgaa gagatgaatg
    14461 atattctcag cctgctccct gattggcttg tcgcggtgct tgttgtacgc ggagaggacc
    14521 ttatccagat tcgcgtcggc caggatcacg cgcttggaga actcggaaat ctgctcaatg
    14581 atctcgtcga ggtaatgctt gtgctgctcg acgaacagct gcttctgctc gttgtcctcg
    14641 gggctgccct tgagcttctc gtagtgggag gccaggtaga ggaagttcac atacttggac
    14701 ggcagagcca gctcgttccc cttctgcagc tcgccagcgg aagccagcat ccgcttcctg
    14761 ccgttctcca gctcgaagag tgagtacttg gggagcttaa tgatcaggtc cttcttcacc
    14821 tccttgtagc ccttcgcctc caggaaatcg atcgggttct tctcgaagct ggagcgctcc
    14881 ataatcgtga tccccagcag ctccttcacg ctcttgagct tcttggactt gcccttctca
    14941 accttcgcca caaccaggac cgagtaggcc acagtggggc tgtcgaaccc gccgtacttc
    15001 ttcggatccc agtccttctt gcgggcgatg agcttgtcgc tgttccgctt aggcagaatt
    15061 gactccttag agaacccgcc agtctggacc tctgtcttct tgacgatatt cacttgtggc
    15121 atggagagaa ccttcctgac ggtcgcgaaa tccctgccct tgtcccacac gatctccccc
    15181 gtctcgccgt tcgtctcgat gagggggcgc ttccggatct cgccattggc cagagtgatc
    15241 tctgtcttga agaaattcat aatgttagag tagaagaagt acttggcggt agccttgcca
    15301 atctcctgct ccgacttggc gatcatcttc ctcacatcgt aaaccttgta gtccccgtac
    15361 acgaactcgc tctcgagctt tgggtacttc ttgatcagag ctgtgccgac caccgcgttc
    15421 aggtacgcgt catgggcatg gtggtaattg ttgatctccc gaaccttgta gaactggaaa
    15481 tccttcctga agtcggagac gagctttgac ttcagggtga tgaccttcac ctcgcggatc
    15541 agcttgtcat tctcatcgta cttagtgttc atccgtgagt cgagaatctg cgcaacgtgc
    15601 ttagtgatct gccgtgtctc gaccagctgc ctcttgatga agcccgcctt gtccagctca
    15661 gagagcccgc ccctctcagc ctttgtgagg ttatcgaact tccgctgcgt gatcagcttg
    15721 gcattcagga gctggcgcca gtagttcttc atcttcttaa cgacctcctc tgaaggaaca
    15781 ttatcagact tgccccggtt cttgtccgac ctggtgagga ccttgttgtc aatggagtca
    15841 tccttcagga atgactgtgg aacgatatga tcgacgtcgt aatcgctgag cctgttaata
    15901 tccagctcct ggtccacata catatcgcgg ccattctgga ggtagtacag gtagagcttc
    15961 tcattctgca gctgcgtgtt ctccaccggg tgctccttga ggatctggga ccccagctcc
    16021 ttaatgccct cctcgatcct cttcatcctc tcgcgtgagt tcttctggcc cttctgcgtg
    16081 gtctgattct cccgggccat ctcaatgacg atgttctcag gcttgtgcct gcccatgacc
    16141 ttcaccagct cgtccacaac cttcacggtc tgcagaatcc ccttcttgat agctggcgag
    16201 ccagcgaggt tcgcgatatg ctcgtgcagc gagtccccct ggccgctcac ctgagccttc
    16261 tggatatcct ccttgaatgt gaggctgtca tcgtgaatca gctgcatgaa attgcggttc
    16321 gcgaagccat cgctcttcag gaagtcgagg atcgtcttcc cggactgctt gtcccgaatg
    16381 ccgttgatga gcttcctgct cagcctcccc cagccggtgt acctcctcct cttgagctgc
    16441 ttcatgacct tgtcatcgaa gagatgggcg taagtcttca ggcgctcctc gatcatctcc
    16501 cggtcctcga acagagtgag tgtcagcaca atgtcctcga ggatatcctc attctcctcg
    16561 ttgtccagga agtccttatc cttaatgatc ttcaggagat cgtggtaggt ccccagggag
    16621 gcgttgaagc ggtcctcaac gccagagatc tcgaccgaat cgaagcactc aatcttcttg
    16681 aagtagtcct ccttgagctg cttaaccgtg accttccggt tggtcttgaa caggaggtcc
    16741 acgatggcct tcttctgctc cccagacagg aaagccggct tcctcatgcc ctcggtcaca
    16801 tacttcacct tagtcagctc gttgtagact gtgaagtact cgtacaggag cgagtgctta
    16861 gggagcacct tctcatttgg caggttcttg tcgaaattcg tcatcctctc gatgaacgac
    16921 tgagcgctag cgcccttgtc gaccacctcc tcgaagttcc acggcgtgat cgtctcctct
    16981 gacttgcggg tcatccaagc gaagcgggag ttgcccctag cgagtgggcc gacgtagtac
    17041 gggatcctga aagtcagaat cttctcgatc ttctcgcggt tatccttgag gaaagggtag
    17101 aagtcctcct gcctcctcag gatagcgtgc agctccccga gatgaatctg gtgtgggatg
    17161 ctgccgttat cgaatgtccg ctgcttcctc aggaggtcct cgcgattgag cttcaccagc
    17221 agctcctccg tgccgtccat cttctccaga atcggcttga tgaacttgta gaactcctcc
    17281 tgagaggccc cgccgtcaat gtacccagcg tagccgttct tcgactgatc gaagaagatc
    17341 tccttgtact tctcggggag ctgctgcctg accagcgcct tcaggagggt cagatcctga
    17401 tggtgctcgt cgtagcgctt gatcatggag gctgagagcg gagccttcgt aatctcggtg
    17461 ttcaccctga gaatatcaga caggaggatg gcgtccgaca gattcttggc agcgaggaac
    17521 aggtccgcgt actgatcgcc gatctgggcc aggaggttat ccaggtcatc gtcgtatgtg
    17581 tccttggaga gctgcagctt ggcgtcctca gcgagatcga aattcgactt gaagttgggc
    17641 gtgagcccca ggctgagcgc aatgagattc ccgaacaggc cgttcttctt ctcgcccggc
    17701 agctgggcga tcaggttctc gaggcgccga gacttcgaga gcctagcgga caggatagcc
    17761 ttcgcgtcga cgcctgacgc attaatgggg ttctcctcga agagctggtt gtacgtctgc
    17821 acgagctgga tgaacagctt gtcaacatcg ctattgtccg ggttgagatc cccctcgatc
    17881 aggaaatggc ccctgaactt aatcatgtgg gccagagcga ggtagatcag gcggaggtcc
    17941 gccttatctg tggagtccac gagcttcttc cgcagatggt agatcgtagg gtacttctcg
    18001 tggtaggcaa cctcgtcgac aatgttgccg aagattggat gccgctcgtg cttcttatcc
    18061 tcctccacga ggaatgactc ctccagcctg tggaagaaag aatcgtcaac cttcgccatc
    18121 tcgttggaga aaatctcctg caggtagcag atgcgattct tcctgcgcgt gtaccgcctg
    18181 cgggcggtgc gcttgagccg cgtagcctca gccgtctcgc cgctgtcgaa caggagagcg
    18241 ccaatgagat tcttcttgat ggaatgccga tcggtgttgc ccaggacctt gaacttcttt
    18301 gagggcacct tgtactcgtc ggtgatcacg gcccagccaa cagagttagt cccaatagcg
    18361 aggccgatcg agtacttctt gtcagcagct ggcaccccgt ggatgccaac cttcctcttc
    18421 ttcttcggag ccatcgaacc cccgctgctg cccccgctgc tttcaggtgt tgcgctctcg
    18481 cttgtgccag gtgtctcaga gccagaggag cctccgctag atcctccgga gttgatggag
    18541 ctctgggcct tcttctggct gttgaacacc tgtctaggca tccgatagaa atcgcacagc
    18601 agggcggcac attcatctgc caggattccc tcggtaattt cgacgcggtg attcatgccg
    18661 gggtagttca gcacgttcat cagggagcct gcggcgcctc tttttgagtt cctcacgcca
    18721 aacaccacgc ggccgatcct agagttgatc atggcgccgg cgcacatcac gcaaggctcg
    18781 aatgtcacgt acagggtggc gtcaaagagt ctgtagttct gcatgaccag gccgccctgt
    18841 ctcagggcca taatttcggc atgggctgtt gggtcgtgca ggccgatggc tctgttccag
    18901 ccctcgccga tcactctatt gttcagcacc agcacggctc ccacaggccc cgcgccttca
    18961 tcccgtgccc tcttggccag ggtcagggca tgtctcatcc agtactcgtg ggaaaactcc
    19021 acctcagaga ctttccgctt cttctttggt gactcgaact cgcttccgtc ggctgtccgt
    19081 ttcattcctg caggctgcag aagtaacacc aaacaacagg gtgagcatcg acaaaagaaa
    19141 cagtaccaag caaataaata gcgtatgaag gcagggctaa aaaaatccac atatagctgc
    19201 tgcatatgcc atcatccaag tatatcaaga tcaaaataat tataaaacat acttgtttat
    19261 tataatagat aggtactcaa ggttagagca tatgaataga tgctgcatat gccatcatgt
    19321 atatgcatca gtaaaaccca catcaacatg tatacctatc ctagatcgat atttccatcc
    19381 atcttaaact cgtaactatg aagatgtatg acacacacat acagttccaa aattaataaa
    19441 tacaccaggt agtttgaaac agtattctac tccgatcttg aacgaatgaa cgaccgccca
    19501 accacaccac atcatcacaa ccaagcgaac aaaaagcatc tctgtatatg catcagtaaa
    19561 acccgcatca acatgtatac ctatcctaga tcgatatttc catccatcat cttcaattcg
    19621 taactatgaa tatgtatggc acacacatac agatccaaaa ttaataaatc caccaggtag
    19681 tttgaaacag aattaattct actccgatct tgaacgaccg cccaaccaga ccacatcatc
    19741 acaaccaaga caaaaaaaag catgaaaaga tgacccgaca aacaagtgca cggcatatat
    19801 tgaaataaag gaaaagggca aaccaaaccc tatgcaacga aacaaaaaaa atcatgaaat
    19861 cgatcccgtc tgcggaacgg ctagagccat cccaggattc cccaaagaga aacactggca
    19921 agttagcaat cagaacgtgt ctgacgtaca ggtcgcatcc gtgtacgaac gctagtagca
    19981 cggatctaac acaaacacgg atctaacaca aacatgaaca gaagtagaac taccgggccc
    20041 taaccatgga ccggaacgcc gatcttgaga aggtagagag gggggggggg gaggacgagc
    20101 ggcgtacctt gaagcggagg tgccgacggg tggatttggg ggagttctgg ttgtgtgtgt
    20161 gtgcgctccg aacaacacga ggttggggaa agagggtgtg gagggggtgt ctatttatta
    20221 cggcgggcga ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg tagctgccgg
    20281 tgccgtgaga ggaggaggag gccgcctgcc gtgccggctc acgtctgccg ctccgccacg
    20341 caatttctgg atgccgacag cggagcaagt ccaacggtgg agcggaactc tcgagagggg
    20401 tccagaggca gcgacagaga tgccgtgccg tctgcttcgc ttggcccgac gcgacgctgc
    20461 tggttcgctg gttggtgtcc gttagactcg tcgacggcgt ttaacaggct ggcattatct
    20521 actcgaaaca agaaaaatgt ttccttagtt tttttaattt cttaaagggt atttgtttaa
    20581 tttttagtca ctttatttta ttctatttta tatctaaatt attaaataaa aaaactaaaa
    20641 tagagtttta gttttcttaa tttagaggct aaaatagaat aaaatagatg tactaaaaaa
    20701 attagtctat aaaaaccatt aaccctaaac cctaaatgga tgtactaata aaatggatga
    20761 agtattatat aggtgaagct atttgcaaaa aaaaaggaga acacatgcac actaaaaaga
    20821 taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc tttagaccat gtctaactgt
    20881 tcatttatat gattctctaa aacactgata ttattgtagt actatagatt atattattcg
    20941 tagagtaaag tttaaatata tgtataaaga tagataaact gcacttcaaa caagtgtgac
    21001 aaaaaaaata tgtggtaatt ttttataact tagacatgca atgctcacct tatctcaaga
    21061 gaggggcacg accgggtcac gctgcactgc agctccggcg cgccgatccc cgggtaccga
    21121 gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    21181 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    21241 ctaactcaca ttaattgcgt tgcgct
//
