LOCUS       sequence-430966-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pX330 expressing Cas9 and a sgRNA targeting the LC3B locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Broadbent DG, Barnaba C, Perez GI, Schmidt JC
  TITLE     Quantitative analysis of autophagy reveals the role of ATG9 and ATG2
            in autophagosome formation.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202210078. doi: 
            10.1083/jcb.202210078. Epub 2023 Apr 28.
  PUBMED    37115157
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202210078. doi"; pages: 
            " 10.1083/jcb.202210078. Epub 2023 Apr 28"
COMMENT     Sequence Label: LC3B sgRNA
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1097..1117)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1124..1189)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      1189..1198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1210..1437
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     promoter        complement(1438..1714)
                     /label=chicken beta-actin promoter
     enhancer        1716..2001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(2098..2173)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2195..2443)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2253..2272)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2423..2443)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(2505..3093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2585..2604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3264..4055,4056..4124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3887..3906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4125..4229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4297..4315
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4353..4375)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4475..4494
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4511..4966)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4648..4669)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4860..4879
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5041..5181
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5052..5181
                     /label=AAV2 ITR
     polyA_signal    5190..5397
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5386..5403
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5431..5478)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(5479..8507,1..1072))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(5479..8507,1..1072))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 agccgttctt gctctggtcg aagaaaatct ctttgtactt ctcaggcagc tgctgccgca
       61 cgagagcttt cagcagggtc aggtcctggt ggtgctcgtc gtatctcttg atcatagagg
      121 cgctcagggg ggccttggtg atctcggtgt tcactctcag gatgtcgctc agcaggatgg
      181 cgtcggacag gttcttggcg gccagaaaca ggtcggcgta ctggtcgccg atctgggcca
      241 gcaggttgtc caggtcgtcg tcgtaggtgt ccttgctcag ctgcagtttg gcatcctcgg
      301 ccaggtcgaa gttgctcttg aagttggggg tcaggcccag gctcagggca atcaggtttc
      361 cgaacaggcc attcttcttc tcgccgggca gctgggcgat cagattttcc agccgtctgc
      421 tcttgctcag tctggcagac aggatggcct tggcgtccac gccgctggcg ttgatggggt
      481 tttcctcgaa cagctggttg taggtctgca ccagctggat gaacagcttg tccacgtcgc
      541 tgttgtcggg gttcaggtcg ccctcgatca ggaagtggcc ccggaacttg atcatgtggg
      601 ccagggccag atagatcagc cgcaggtcgg ccttgtcggt gctgtccacc agtttctttc
      661 tcaggtggta gatggtgggg tacttctcgt ggtaggccac ctcgtccacg atgttgccga
      721 agatggggtg ccgctcgtgc ttcttatcct cttccaccag gaaggactct tccagtctgt
      781 ggaagaagct gtcgtccacc ttggccatct cgttgctgaa gatctcttgc agatagcaga
      841 tccggttctt ccgtctggtg tatcttcttc tggcggttct cttcagccgg gtggcctcgg
      901 ctgtttcgcc gctgtcgaac agcagggctc cgatcaggtt cttcttgatg ctgtgccggt
      961 cggtgttgcc cagcaccttg aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg
     1021 cccagcccac agagttggtg ccgatgtcca ggccgatgct gtacttcttg tcggctgctg
     1081 ggactccgtg gataccgacc ttccgcttct tctttggggc catcttatcg tcatcgtctt
     1141 tgtaatcaat atcatgatcc ttgtagtctc cgtcgtggtc cttatagtcc atggtggcac
     1201 cggtccaacc tgaaaaaaag tgatttcagg caggtgctcc aggtaattaa acattaatac
     1261 cccaccaacc aaccatccct taaaccctta cctcttgctc agctaattac agcccggagg
     1321 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     1381 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     1441 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     1501 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg
     1561 cccctcgccc cgccccgccc cgcctggcgc gcgccccccc ccccccccgc ccccatcgct
     1621 gcacaaaata attaaaaaat aaataaatac aaaattgggg gtggggaggg gggggagatg
     1681 gggagagtga agcagaacgt ggggctcacc tcgaccatgg taatagcgat gactaatacg
     1741 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcaca atgccaggcg
     1801 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     1861 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     1921 attggcgtta ctattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     1981 tttaccgtaa gttatgtaac gggtacctct agagccattt gtctgcagaa ttggcgcacg
     2041 cgctaaaaac ggactagcct tattttaact tgctatttct agctctaaaa caaaaaagca
     2101 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
     2161 ctagctctaa aactgccgtc ggagaagacc ttccggtgtt tcgtcctttc cacaagatat
     2221 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
     2281 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
     2341 gtactaatat ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt
     2401 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcacatgtg agcaaaaggc
     2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3241 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3301 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3361 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcggcttcc acgctcaccg
     3421 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3481 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3541 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3601 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3661 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3721 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3781 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3841 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3901 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3961 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4021 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4081 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4261 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4321 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4381 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4441 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4501 gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4561 atcagctcat tttttaacca atagaccgaa atcggcaaaa tcccttataa atcaaaagaa
     4621 tagcccgaga tagagttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4681 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4741 ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4801 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4861 gggaagaaag cgaaaggagc gggcgctaag gcgctggcaa gtgtagcggt cacgctgcgc
     4921 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt
     4981 gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     5041 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
     5101 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
     5161 actccatcac taggggttcc tgcggccgct ccccagcatg cctgctattc tcttcccaat
     5221 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     5281 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     5341 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     5401 aggctgatca gcgagctcta ggaattctta ctttttcttt tttgcctggc cggccttttt
     5461 cgtggccgcc ggccttttgt cgcctcccag ctgagacagg tcgatccgtg tctcgtacag
     5521 gccggtgatg ctctggtgga tcagggtggc gtccagcacc tctttggtgc tggtgtacct
     5581 cttccggtcg atggtggtgt caaagtactt gaaggcggca ggggctccca gattggtcag
     5641 ggtaaacagg tggatgatat tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt
     5701 gtaggcggac agcactttgt ccagattagc gtcggccagg atcactctct tggagaactc
     5761 gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc tgttccacaa acagctgttt
     5821 ctgctcatta tcctcggggg agcccttcag cttctcatag tggctggcca ggtacaggaa
     5881 gttcacatat ttggagggca gggccagttc gtttcccttc tgcagttcgc cggcagaggc
     5941 cagcattctc ttccggccgt tttccagctc gaacagggag tacttaggca gcttgatgat
     6001 caggtccttt ttcacttctt tgtagccctt ggcttccaga aagtcgatgg gattcttctc
     6061 gaagctgctt ctttccatga tggtgatccc cagcagctct ttcacactct tcagtttctt
     6121 ggacttgccc ttttccactt tggccaccac cagcacagaa taggccacgg tggggctgtc
     6181 gaagccgccg tacttcttag ggtcccagtc cttctttctg gcgatcagct tatcgctgtt
     6241 cctcttgggc aggatagact ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac
     6301 gatattcact tggggcatgc tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc
     6361 ccacacgatc tccccggttt cgccgtttgt ctcgatcaga ggccgcttcc ggatctcgcc
     6421 gttggccagg gtaatctcgg tcttgaaaaa gttcatgatg ttgctgtaga agaagtactt
     6481 ggcggtagcc ttgccgattt cctgctcgct cttggcgatc atcttccgca cgtcgtacac
     6541 cttgtagtcg ccgtacacga actcgctttc cagcttaggg tactttttga tcagggcggt
     6601 tcccacgacg gcgttcaggt aggcgtcgtg ggcgtggtgg tagttgttga tctcgcgcac
     6661 tttgtaaaac tggaaatcct tccggaaatc ggacaccagc ttggacttca gggtgatcac
     6721 tttcacttcc cggatcagct tgtcattctc gtcgtactta gtgttcatcc gggagtccag
     6781 gatctgtgcc acgtgctttg tgatctgccg ggtttccacc agctgtctct tgatgaagcc
     6841 ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt cgaactttct
     6901 ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct tcttcacgac
     6961 ctcttcggag ggcacgttgt cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt
     7021 gttgtcgatg gagtcgtcct tcagaaagct ctgaggcacg atatggtcca catcgtagtc
     7081 ggacagccgg ttgatgtcca gttcctggtc cacgtacata tcccgcccat tctgcaggta
     7141 gtacaggtac agcttctcgt tctgcagctg ggtgttttcc acggggtgtt ctttcaggat
     7201 ctggctgccc agctctttga tgccctcttc gatccgcttc attctctcgc ggctgttctt
     7261 ctgtcccttc tgggtggtct ggttctctct ggccatttcg atcacgatgt tctcgggctt
     7321 gtgccggccc atcactttca cgagctcgtc caccaccttc actgtctgca ggatgccctt
     7381 cttaatggcg gggctgccgg ccagattggc aatgtgctcg tgcaggctat cgccctggcc
     7441 ggacacctgg gctttctgga tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg
     7501 catgaagttt ctgttggcga agccgtcgga cttcaggaaa tccaggattg tcttgccgga
     7561 ctgcttgtcc cggatgccgt tgatcagctt ccggctcagc ctgccccagc cggtgtatct
     7621 ccgccgcttc agctgcttca tcactttgtc gtcgaacagg tgggcatagg ttttcagccg
     7681 ttcctcgatc atctctctgt cctcaaacag tgtcagggtc agcacgatat cttccagaat
     7741 gtcctcgttt tcctcattgt ccaggaagtc cttgtccttg ataattttca gcagatcgtg
     7801 gtatgtgccc agggaggcgt tgaaccgatc ttccacgccg gagatttcca cggagtcgaa
     7861 gcactcgatt ttcttgaagt agtcctcttt cagctgcttc acggtcactt tccggttggt
     7921 cttgaacagc aggtccacga tggccttttt ctgctcgccg ctcaggaagg cgggctttct
     7981 cattccctcg gtcacgtatt tcactttggt cagctcgtta tacacggtga agtactcgta
     8041 cagcaggctg tgcttgggca gcaccttctc gttgggcagg ttcttatcga agttggtcat
     8101 ccgctcgatg aagctctggg cggaagcgcc cttgtccacc acttcctcga agttccaggg
     8161 ggtgatggtt tcctcgctct ttctggtcat ccaggcgaat ctgctgtttc ccctggccag
     8221 agggcccacg tagtagggga tgcggaaggt caggatcttc tcgatctttt cccggttgtc
     8281 cttcaggaat gggtaaaaat cttcctgccg ccgcagaatg gcgtgcagct ctcccaggtg
     8341 gatctggtgg gggatgctgc cgttgtcgaa ggtccgctgc ttccgcagca ggtcctctct
     8401 gttcagcttc acgagcagtt cctcggtgcc gtccatcttt tccaggatgg gcttgatgaa
     8461 cttgtagaac tcttcctggc tggctccgcc gtcaatgtag ccggcgt
//
