LOCUS       sequence-43097-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Zhao Z, Zuber J, Diaz-Flores E, Lintault L, Kogan SC, Shannon K, 
            Lowe SW
  TITLE     p53 loss promotes acute myeloid leukemia by enabling aberrant 
            self-renewal.
  JOURNAL   Genes Dev. 2010 Jul 1;24(13):1389-402.
  PUBMED    20595231
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2010-07-1"; volume: "24(13)"; pages: "1389-402"
COMMENT     Sequence Label: LGmCreER (self deleting)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           81..174
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           188..300
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(326..359)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(405..428)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gacgagctgt acaagtaatg aattaattaa gaattatcaa gcttatcgat accgtcgact
       61 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
      121 catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagca
      181 accagaattc aaggggctac tttaggagca attatcttgt ttactaaaac tgaatacctt
      241 gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt aaatcacttt
      301 tttcaattgg aagactaatg cattgataac ttcgtatagt atacattata cgaagttatc
      361 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      421 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      481 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      541 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      601 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      661 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      721 tgggaagggg tgggtccggg ggcgggctca ngggcgggct caggggcggg gcgggcgccc
      781 ga
//
