LOCUS       sequence-430976-        6158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of RiboL1-jGCaMP8s (soma targeted) in striatal indirect 
            pathway medium spiny neurons (D2 MSNs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP14872:
            pAAV-AiE0452h_3xC2-minBG-RiboL1-jGCa...
FEATURES             Location/Qualifiers
     source          1..6158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1333..1354)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1594..1613)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2985..3005)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    3192..3303
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3421..3561
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3421..3550
                     /label=AAV2 ITR
     rep_origin      3636..4091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3723..3742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3933..3954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4108..4127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4227..4249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4287..4305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4373..4477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4478..4546,4547..5338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4696..4715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5509..6097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5998..6017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgaaa aatacctagt cttgaacttt ggagttccaa
      181 attccacatg atcgacttcc tcaaggcttt tcacttgacg aacaaattgc atgccaagaa
      241 atcgtcaaaa ttctatcaag cttaattatg caatttctgt ctataaatga catacctcct
      301 gtaccctatg acacccacct atgcactaat aagatgtgac agctgcatca ccaacaaagc
      361 ctcccccgcc tggtgaaaaa tacctagtct tgaactttgg agttccaaat tccacatgat
      421 cgacttcctc aaggcttttc acttgacgaa caaattgcat gccaagaaat cgtcaaaatt
      481 ctatcaagct taattatgca atttctgtct ataaatgaca tacctcctgt accctatgac
      541 acccacctat gcactaataa gatgtgacag ctgcatcacc aacaaagcct cccccgcctg
      601 gtgaaaaata cctagtcttg aactttggag ttccaaattc cacatgatcg acttcctcaa
      661 ggcttttcac ttgacgaaca aattgcatgc caagaaatcg tcaaaattct atcaagctta
      721 attatgcaat ttctgtctat aaatgacata cctcctgtac cctatgacac ccacctatgc
      781 actaataaga tgtgacagct gcatcaccaa caaagcctcc cccgcctgga gctcgggctg
      841 ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga tccacccgcc
      901 accatgcatc atcaccatca tcacacgcgt cgcaagaaga ccttcaagga ggtggccacc
      961 gccgtgaaga tcatcgccat gctgatgggc ctgaagatca acgtctatat caaggccgac
     1021 aagcagaaga acggcatcaa ggcgaacttc cacatccgcc acaacatcga ggacggcggc
     1081 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cgtggagtcc aaactttcga aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
     1321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1381 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1501 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1561 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acctgccgga ccaactgact
     1741 gaagagcaga tcgcagaata caaagaggct ttctccctat ttgacaagga cggggatggg
     1801 acaataacaa ccaaggagct ggggacggtg atgcggtctc tcggacacaa ccccacagaa
     1861 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc
     1921 cctgagttcc tgacaatgat ggcaagaaaa atgaaataca gggacacgga agaagaaatt
     1981 agagaagcgt tcggtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt
     2041 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc
     2101 agggaagcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     2161 acagcgaagg ggggcagcgg tgggtctgga gggactgggg gtagcggtgg tagcggtggg
     2221 acagggggaa gcggcggtag tggtggaaca agcagcaaag tctcacgcga caccctgtac
     2281 gaggcggtgc gggaagtcct gcacgggaac cagcgcaagc gccgcaagtt tctggagacg
     2341 gtggagctgc agatcagcct gaagaactac gaccctcaga aggacaaacg tttctcgggc
     2401 accgtcaggc tcaagtccac cccacgcccc aagttctcgg tgtgcgttct gggggaccag
     2461 cagcactgtg atgaagccaa ggccgtggat atcccccaca tggacatcga ggcgctcaag
     2521 aaacttaaca aaaacaagaa gttggtcaag aagctggcta agaagtacga tgcctttttg
     2581 gcctctgagt ctctgattaa gcagatccca cgtatcctgg gcccaggcct aaacaaggct
     2641 ggcaagttcc cctccctgct gacacacaat gaaaacatgg tggccaaagt ggatgaggtg
     2701 aaatcgacaa tcaagttcca gatgaagaag gtgctgtgtt tggccgtcgc tgttggccac
     2761 gtgaagatga ccgatgatga gctagtctac aacattcatc tggctgtcaa tttcttggtg
     2821 tccttgctta agaaaaactg gcaaaacgtg cgggctctgt acatcaagag caccatgggc
     2881 aagccccagc gtctgtatta gggcgcgccg cggccgcgaa ttcgatatca taatcaacct
     2941 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3001 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3061 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
     3121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggctcga
     3181 gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3241 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3301 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3361 gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc gagcggccgc
     3421 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     3481 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     3541 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     3601 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     3661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     3721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3781 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3841 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3901 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3961 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     4021 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4081 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4141 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4201 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4261 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4321 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4381 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4441 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4501 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4561 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4621 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4681 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4741 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4801 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4861 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4921 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4981 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5041 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5101 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5161 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5221 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5281 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6121 acggttcctg gccttttgct ggccttttgc tcacatgt
//
