LOCUS       sequence-43100-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Zhao Z, Zuber J, Diaz-Flores E, Lintault L, Kogan SC, Shannon K, 
            Lowe SW
  TITLE     p53 loss promotes acute myeloid leukemia by enabling aberrant 
            self-renewal.
  JOURNAL   Genes Dev. 2010 Jul 1;24(13):1389-402.
  PUBMED    20595231
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2010-07-1"; volume: "24(13)"; pages: "1389-402"
COMMENT     Sequence Label: LGshp53CreER (self deleting)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           75..196
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           224..238
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           266..392
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(418..451)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(497..520)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctgtacaagt aatgaattaa ttaagaatta tcaagcttat cgataccgtc gactagggat
       61 aacagggtaa ttgtttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt
      121 ggaaacactt gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata
      181 ttgctgttga cagtgagcgc ccactacaag tacatgtgta atagtgaagc cacagatgta
      241 ttacacatgt acttgtagtg gatgcctact gcctcggaat tcaaggggct actttaggag
      301 caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag
      361 ctgaattaaa atggtataaa ttaaatcact tttttcaatt ggaagactaa tgcattgata
      421 acttcgtata gtatacatta tacgaagtta tcaattctac cgggtagggg aggcgctttt
      481 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
      541 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
      601 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
      661 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
      721 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
      781 gctttgctcc ttcgctttct gggctcanag gctgggaagg ggtgggtccg ggggcgggct
      841 caggggcgg
//
