LOCUS       sequence-431008-        2133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GFP tagged B55 in mammalian cells; tev cleavable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, Nilsson J, 
            Kelker MS, Page R, Peti W
  TITLE     Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
  JOURNAL   Nature. 2024 Jan;625(7993):195-203. doi: 10.1038/s41586-023-06870-3.
            Epub 2023 Dec 20.
  PUBMED    38123684
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06870-3"; journalName: "Nature"; date: 
            "2024-01"; volume: "625"; issue: "7993"; pages: "195-203"
COMMENT     Sequence Label: pcDNA3.4-GFP-TEV-B55
FEATURES             Location/Qualifiers
     source          1..2133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             760..780
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtatacca tgggctctga taaaattatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagggagca ggtgctggag aaaatcttta ttttcaaggt
      781 catatgggat ccgcaggagc tggaggaggg aatgatattc agtggtgttt ttctcaggtg
      841 aaaggagcag tagatgatga tgtagcagaa gcagatataa tttctacagt agaatttaat
      901 cattctggag aattactagc aacaggagat aaaggtggta gagttgtcat ctttcaacag
      961 gagcaggaga acaaaatcca gtctcatagc agaggagaat ataatgttta cagcaccttc
     1021 cagagccatg aaccagagtt tgactacttg aaaagtttag aaatagaaga aaagatcaac
     1081 aaaattaggt ggttacccca gaaaaatgct gctcagtttt tattgtctac caatgataaa
     1141 acaataaaat tatggaaaat cagtgaaagg gacaaaagac cagaagggta taacttgaaa
     1201 gaggaggatg gaaggtatag agatcctact acagttacta cactacgagt gccagtcttt
     1261 aggcctatgg atctaatggt tgaggccagt ccacgaagaa tatttgccaa tgctcataca
     1321 tatcacatca actcaatttc tattaatagt gattatgaaa catatttatc tgcagatgat
     1381 ttgcggatta atctttggca tctggaaatt acagacagga gttttaacat tgtggatatc
     1441 aagcctgcca atatggaaga gctaacagag gtgattacag cagcagaatt tcatccaaac
     1501 agctgtaaca catttgtata cagcagcagt aaaggaacta ttcggctatg tgacatgagg
     1561 gcatctgccc tctgtgatag acattctaaa ttgtttgaag aacctgaaga tcccagtaac
     1621 aggtcatttt tttccgaaat catctcctct atttcggatg taaaattcag ccatagtggt
     1681 cgatatatga tgactagaga ctatttgtca gtcaaaattt gggacttaaa tatggaaaac
     1741 aggcctgtgg aaacatacca ggtgcatgaa tacctcagaa gtaaactctg ttcactgtat
     1801 gaaaatgact gcatatttga caaatttgaa tgttgttgga atggatctga cagtgttgtc
     1861 atgactggat cttacaataa tttcttcaga atgtttgaca gaaacacaaa gcgagacata
     1921 accctagaag catcgcggga aaacaataag cctcgcacag ttctgaagcc tcgcaaagtc
     1981 tgtgcaagtg gcaagcgaaa gaaagatgaa ataagtgttg acagcctaga cttcaataag
     2041 aaaatccttc acacagcctg gcaccccaag gaaaatatca ttgccgtagc tactacaaac
     2101 aatctgtata tatttcaaga caaagtgaat tga
//
