LOCUS       sequence-431010-        8737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector for expression of MEK1 E102-I103deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8737)
  TITLE     Der Lab MEK1 Plasmids 2024
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MEK1-E102-I103del-V5
FEATURES             Location/Qualifiers
     source          1..8737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..226
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        38..234
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      85..220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             266..868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(266..285)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     762..782
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    911..1028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1086..1586
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1505..1523
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2774..2815
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2840..3428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2893..2913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3311..3322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3500..3733
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3811..3932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3848..3867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3902..3921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3972..4107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4127..4146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4128..4146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4156..4173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4156..4172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4165..4187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4314..4769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4401..4420)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4611..4632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4795..4899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4900..4968,4969..5760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5118..5137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5931..6519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6420..6439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6673..6690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6843..6848,6849..6866,6867..6873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6886..6908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6905..6921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6905..6921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6940..6960
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6942..6960
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6988..7214
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7215..7395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7442..7567
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8060..8293
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8478..8522
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
       61 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      121 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
      241 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
      301 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
      361 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
      421 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
      481 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
      541 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
      601 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
      661 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
      721 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
      781 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
      841 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
      901 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      961 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1021 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     1081 gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1141 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1201 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1261 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1321 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1381 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     1441 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     1501 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     1561 accgaatcac cgacctctct ccccaggggc tagcatgccc aagaagaagc cgacgcccat
     1621 ccagctgaac ccggcccccg acggctctgc agttaacggg accagctctg cggagaccaa
     1681 cttggaggcc ttgcagaaga agctggagga gctagagctt gatgagcagc agcgaaagcg
     1741 ccttgaggcc tttcttaccc agaagcagaa ggtgggagaa ctgaaggatg acgactttga
     1801 gaagatcagt gagctggggg ctggcaatgg cggtgtggtg ttcaaggtct cccacaagcc
     1861 ttctggcctg gtcatggcca gaaagctaat tcatctgaaa cccgcaatcc ggaaccagat
     1921 cataagggag ctgcaggttc tgcatgagtg caactctccg tacatcgtgg gcttctatgg
     1981 tgcgttctac agcgatggcg agatcagtat ctgcatggag cacatggatg gaggttctct
     2041 ggatcaagtc ctgaagaaag ctggaagaat tcctgaacaa attttaggaa aagttagcat
     2101 tgctgtaata aaaggcctga catatctgag ggagaagcac aagatcatgc acagagatgt
     2161 caagccttcc aacatcctag tcaactcccg tggggagatc aagctctgtg actttggggt
     2221 cagcgggcag ctcatcgact ccatggccaa ctccttcgtg ggcacaaggt cctacatgtc
     2281 gccagaaaga ctccagggga ctcattactc tgtgcagtca gacatctgga gcatgggact
     2341 gtctctggta gagatggcgg ttgggaggta tcccatccct cctccagatg ccaaggagct
     2401 ggagctgatg tttgggtgcc aggtggaagg agatgcggct gagaccccac ccaggccaag
     2461 gacccccggg aggcccctta gctcatacgg aatggacagc cgacctccca tggcaatttt
     2521 tgagttgttg gattacatag tcaacgagcc tcctccaaaa ctgcccagtg gagtgttcag
     2581 tctggaattt caagattttg tgaataaatg cttaataaaa aaccccgcag agagagcaga
     2641 tttgaagcaa ctcatggttc atgcttttat caagagatct gatgctgagg aagtggattt
     2701 tgcaggttgg ctctgctcca ccatcggcct taaccagccc agcaccccaa cccatgctgc
     2761 tggcgtcacc ggtggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     2821 atgaccgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2881 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2941 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3001 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3061 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3121 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3181 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3241 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3301 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3361 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3421 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3481 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3541 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3601 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3661 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3721 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3781 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3841 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3901 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3961 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4021 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4081 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4141 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4201 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4261 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4321 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4381 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4441 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4501 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4561 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4621 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4681 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4741 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6901 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6961 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7021 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7081 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7141 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7201 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7381 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7441 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7501 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7561 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7621 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7681 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7741 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7801 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7861 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7921 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7981 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8041 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8101 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8161 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8221 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8281 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8341 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8401 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8461 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8521 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8581 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8641 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     8701 attgcatctc aattagtcag caaccaggtg tggaaag
//
