LOCUS       sequence-431040-       10258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human KU70-eGFP-SUN1-Delta-N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Shokrollahi M, Stanic M, Hundal A, Chan JNY, Urman D, Hakem A, 
            Garcia RE, Hao J, Maass PG, Dickson BC, Hande MP, Pujana MA, Hakem 
            R, Mekhail K
  TITLE     DNA double-strand break-capturing nuclear envelope tubules drive DNA
            repair
  JOURNAL   bioRxiv 2023.05.07.539750
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.07.539750"
COMMENT     Sequence Label: KU70-eGFP-SUN1-Delta-N
FEATURES             Location/Qualifiers
     source          1..10258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2791..2811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2827..2829,2830..2832,2833..3543)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2827..2829,2830..2832,2833..3543)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2872..2893)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3133..3152)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3480..3501
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5849..5866)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5855..6079
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6125..6553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6212..6231)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6422..6443
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6562..6582)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6567..6896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6747..6882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6809..6828
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6963..7757
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7017..7036)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7627..7646
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7931..8052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7968..7987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8022..8041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8101..8117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8101..8117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8114..8136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8125..8141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8149..8155,8156..8173,8174..8179))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8194..8215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8332..8349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8503..9091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8583..8602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9262..10053,10054..10122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9885..9904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10123..10227)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgt cagggtggga gtcatattac aaaaccgagg gcgatgaaga agcagaggaa
      961 gaacaagaag agaaccttga agcaagtgga gactataaat attcaggaag agatagtttg
     1021 atttttttgg ttgatgcctc caaggctatg tttgaatctc agagtgaaga tgagttgaca
     1081 ccttttgaca tgagcatcca gtgtatccaa agtgtgtaca tcagtaagat cataagcagt
     1141 gatcgagatc tcttggctgt ggtgttctat ggtaccgaga aagacaaaaa ttcagtgaat
     1201 tttaaaaata tttacgtctt acaggagctg gataatccag gtgcaaaacg aattctagag
     1261 cttgaccagt ttaaggggca gcagggacaa aaacgtttcc aagacatgat gggccacgga
     1321 tctgactact cactcagtga agtgctgtgg gtctgtgcca acctctttag tgatgtccaa
     1381 ttcaagatga gtcataagag gatcatgctg ttcaccaatg aagacaaccc ccatggcaat
     1441 gacagtgcca aagccagccg ggccaggacc aaagccggtg atctccgaga tacaggcatc
     1501 ttccttgact tgatgcacct gaagaaacct gggggctttg acatatcctt gttctacaga
     1561 gatatcatca gcatagcaga ggatgaggac ctcagggttc actttgagga atccagcaag
     1621 ctagaagacc tgttgcggaa ggttcgcgcc aaggagacca ggaagcgagc actcagcagg
     1681 ttaaagctga agctcaacaa agatatagtg atctctgtgg gcatttataa tctggtccag
     1741 aaggctctca agcctcctcc aataaagctc tatcgggaaa caaatgaacc agtgaaaacc
     1801 aagacccgga cctttaatac aagtacaggc ggtttgcttc tgcctagcga taccaagagg
     1861 tctcagatct atgggagtcg tcagattata ctggagaaag aggaaacaga agagctaaaa
     1921 cggtttgatg atccaggttt gatgctcatg ggtttcaagc cgttggtact gctgaagaaa
     1981 caccattacc tgaggccctc cctgttcgtg tacccagagg agtcgctggt gattgggagc
     2041 tcaaccctgt tcagtgctct gctcatcaag tgtctggaga aggaggttgc agcattgtgc
     2101 agatacacac cccgcaggaa catccctcct tattttgtgg ctttggtgcc acaggaagaa
     2161 gagttggatg accagaaaat tcaggtgact cctccaggct tccagctggt ctttttaccc
     2221 tttgctgatg ataaaaggaa gatgcccttt actgaaaaaa tcatggcaac tccagagcag
     2281 gtgggcaaga tgaaggctat cgttgagaag cttcgcttca catacagaag tgacagcttt
     2341 gagaaccccg tgctgcagca gcacttcagg aacctggagg ccttggcctt ggatttgatg
     2401 gagccggaac aagcagtgga cctgacattg cccaaggttg aagcaatgaa taaaagactg
     2461 ggctccttgg tggatgagtt taaggagctt gtttacccac cagattacaa tcctgaaggg
     2521 aaagttacca agagaaaaca cgataatgaa ggttctggaa gcaaaaggcc caaggtggag
     2581 tattcagaag aggagctgaa gacccacatc agcaagggta cgctgggcaa gttcactgtg
     2641 cccatgctga aagaggcctg ccgggcttac gggctgaaga gtgggctgaa gaagcaggag
     2701 ctgctggaag ccctcaccaa gcacttccag gacatgctta agggaggtgg atctggaggt
     2761 ggatctggag gtggatctgg aggtggatct cccaagaaga agaggaaggt gggaggttct
     2821 accggtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3001 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3241 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3301 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3541 aagggaggtt ctgttaactg gattcgtgaa cagaccacag tggaccactt ctggggtctt
     3601 gatgatgatg gtgatcttaa aggtggaaat aaagctgcca ttcagggaaa cggggatgtg
     3661 ggagccgccg ccgccaccgc gcacaacggc ttctcctgca gcaactgcag catgctgtcc
     3721 gagcgcaagg acgtgctcac ggcgcacccc gcggcccccg ggcccgtgtc gagagtttat
     3781 tctagggaca ggaatcaaaa atgcggtgcg tctttctacg tgaataggat tttgtggctg
     3841 gccagataca ctgcatcatc tttttcatca tttttagttc aactttttca agtggtttta
     3901 atgaagctca gttatgaatc agaaaattac aaattgaaaa ctcatgaatc aaaagattgt
     3961 gaatccgaaa gctataagtc aaaaagccat gaatcaaaag gcgacgactg taagggcaag
     4021 aggcacctcg acgcgcacac agccgcccac tcgcagtcgc cacggctgcc cggtcgggca
     4081 gggaccctct ggcacatctg ggcatgtgca ggttacttct tgctgcagat tctgcgcagg
     4141 atcggagctg tgggccaggc tgtgtccagg acggcgtggt cggccctttg gctggccgtg
     4201 gttgctccag ggaaggcagc ctctggagtg ttctggtggc tggggattgg atggtaccag
     4261 tttgttactt tgatttcttg gctgaatgtg tttcttctta ccaggtgcct tcgaaacatc
     4321 tgcaagtttt tagtcttgct catcccactc ttccttttac tagcaggtct ctccttacgg
     4381 ggccagggca atttcttttc gttcttgccc gtgttgaatt gggcaagcat gcatagaaca
     4441 cagcgggtgg atgaccccca ggacgtgttt aaacccacga cttctcgcct gaagcagcct
     4501 ctgcagggtg acagtgaggc ttttccgtgg cattggatga gtggcgtgga gcagcaggtg
     4561 gcctctctgt ctggacagtg ccaccaccat ggtgagaatc tccgagagct gaccactttg
     4621 ctacagaagc tgcaggctcg ggtggaccag atggaaggcg gcgctgccgg gccgtcagct
     4681 tcggtcagag acgctgtggg acagcccccg agggagactg actttatggc ctttcaccaa
     4741 gaacatgaag tgcgtatgtc acacttggaa gatattctgg gaaaactgag agaaaaatct
     4801 gaggccatcc agaaggaact agaacagacc aagcaaaaaa caatcagtgc ggttggtgag
     4861 cagctcctgc ccacggtcga gcacctccag ctggagctgg atcagctaaa gtcagagctg
     4921 tccagctggc gacacgtgaa gaccggctgt gagacagtgg atgccgtaca agaaagagtg
     4981 gacgtgcaag tcagagaaat ggtgaaactc ctgttttccg aagatcagca aggcggttct
     5041 ctggaacagc tgctgcagag gttctcatca cagtttgtga gcaaaggcga cttgcagacg
     5101 atgctgcgag acctgcagct gcagatcctg cggaacgtca cccaccacgt ttccgtgacc
     5161 aagcagctcc caacctcaga agccgtggtg tctgctgtga gcgaggcggg ggcgtctgga
     5221 ataacagagg cgcaagcacg tgccatcgtg aacagcgcct tgaagctgta ttcccaagat
     5281 aagaccggga tggtggactt tgctctggaa tctggtggtg gcagcatctt gagtactcgc
     5341 tgttctgaaa cttacgaaac caaaacggcg ctgatgagtc tgtttgggat cccgctgtgg
     5401 tacttctcgc agtccccgcg cgtggtcatc cagcctgaca tttaccccgg taactgctgg
     5461 gcatttaaag gctcccaggg gtacctggtg gtgaggctct ccatgatgat ccacccagcc
     5521 gccttcactc tggagcacat ccctaagacg ctgtcgccaa caggcaacat cagcagcgcc
     5581 cccaaggact tcgccgtcta tggattagaa aatgagtatc aggaagaagg gcagcttctg
     5641 ggacagttca cgtatgatca ggatggggag tcgctccaga tgttccaggc cctgaaaaga
     5701 cccgacgaca cagctttcca aatagtggaa cttcggattt tttctaactg gggccatcct
     5761 gagtatacct gtctgtatcg gttcagagtt catggcgaac ctgtcaagtg actcgagtct
     5821 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5881 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5941 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6001 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6061 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6121 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6181 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6241 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6301 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6361 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6421 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6481 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6541 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6601 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6721 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6781 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6841 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6901 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6961 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7021 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7081 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7141 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7201 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7261 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7321 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7381 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7441 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7501 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7561 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7621 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7681 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7741 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7801 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7861 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7921 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7981 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8041 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8101 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8161 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8221 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8281 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8941 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9241 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9301 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9361 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9421 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9481 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9541 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9601 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9661 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9721 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9781 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9841 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9901 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9961 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10021 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10081 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
