LOCUS       sequence-431105-       12052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human L-MYC and LIN28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hUL
FEATURES             Location/Qualifiers
     source          1..12052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2860..2925
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    3630..4218
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3683..3703)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4101..4112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4309..4328)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4374..4429
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4428..4447)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4790..4806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4790..4806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4803..4825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4814..4830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4838..4844,4845..4862,4863..4868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4883..4904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4993..5026
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5487..7412
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGXGGGA
                     GGXGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGXGGAGAGGGXGGAGAG
                     GGAGGAGAGGAGGXGXGGXGGXGXGGXGGAGXGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    7580..7613
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7800..9589
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10130..10147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10301..10889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10381..10400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11060..11851,11852..11920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11683..11702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11921..12025)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatggact acgactcgta ccagcactat ttctacgact atgactgcgg ggaggatttc
     1801 taccgctcca cggcgcccag cgaggacatc tggaagaaat tcgagctggt gccatcgccc
     1861 cccacgtcgc cgccctgggg cttgggtccc ggcgcagggg acccggcccc cgggattggt
     1921 cccccggagc cgtggcccgg agggtgcacc ggagacgaag cggaatcccg gggccactcg
     1981 aaaggctggg gcaggaacta cgcctccatc atacgccgtg actgcatgtg gagcggcttc
     2041 tcggcccggg aacggctgga gagagctgtg agcgaccggc tcgctcctgg cgcgccccgg
     2101 gggaacccgc ccaaggcgtc cgccgccccg gactgcactc ccagcctcga agccggcaac
     2161 ccggcgcccg ccgccccctg tccgctgggc gaacccaaga cccaggcctg ctccgggtcc
     2221 gagagcccaa gcgactcgga gaatgaagaa attgatgttg tgacagtaga gaagaggcag
     2281 tctctgggta ttcggaagcc ggtcaccatc acggtgcgag cagaccccct ggatccctgc
     2341 atgaagcatt tccacatctc catccatcag caacagcaca actatgctgc ccgttttcct
     2401 ccagaaagct gctcccaaga agaggcttca gagaggggtc cccaagaaga ggttctggag
     2461 agagatgctg caggggaaaa ggaagatgag gaggatgaag agattgtgag tcccccacct
     2521 gtagaaagtg aggctgccca gtcctgccac cccaaacctg tcagttctga tactgaggat
     2581 gtgaccaaga ggaagaatca caacttcctg gagcgcaaga ggcggaatga cctgcgttcg
     2641 cgattcttgg cgctgaggga ccaggtgccc accctggcca gctgctccaa ggcccccaaa
     2701 gtagtgatcc taagcaaggc cttggaatac ttgcaagccc tggtgggggc tgagaagagg
     2761 atggctacag agaaaagaca gctccgatgc cggcagcagc agttgcagaa aagaattgca
     2821 tacctcactg gctacggaga tctcaaaatt gtcgctcctg tcaaacaaac tcttaacttt
     2881 gatttactca aactggctgg ggatgtagaa agcaatccag gtccactcat gaacaattcg
     2941 cccttcacca tgggctccgt gtccaaccag cagtttgcag gtggctgcgc caaggcggca
     3001 gaagaggcgc ccgaggaggc gccggaggac gcggcccggg cggcggacga gcctcagctg
     3061 ctgcacggtg cgggcatctg taagtggttc aacgtgcgca tggggttcgg cttcctgtcc
     3121 atgaccgccc gcgccggggt cgcgctcgac cccccagtgg atgtctttgt gcaccagagt
     3181 aagctgcaca tggaagggtt ccggagcttg aaggagggtg aggcagtgga gttcaccttt
     3241 aagaagtcag ccaagggtct ggaatccatc cgtgtcaccg gacctggtgg agtattctgt
     3301 attgggagtg agaggcggcc aaaaggaaag agcatgcaga agcgcagatc aaaaggagac
     3361 aggtgctaca actgtggagg tctagatcat catgccaagg aatgcaagct gccaccccag
     3421 cccaagaagt gccacttctg ccagagcatc agccatatgg tagcctcatg tccgctgaag
     3481 gcccagcagg gccctagtgc acagggaaag ccaacctact ttcgagagga agaagaagaa
     3541 atccacagcc ctaccctgct cccggaggca cagaattgaa agggcgaatt gcctgcagga
     3601 attcaagctt cggggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa
     3661 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3721 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3781 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3841 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3901 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3961 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4021 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4081 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4141 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4201 tttgggccgc ctccccgcat cggtacaatt cactcctcag gtgcaggctg cctatcagaa
     4261 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     4321 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     4381 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     4441 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     4501 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     4561 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     4621 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     4681 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     4741 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     4801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4921 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctcaattccg atcatattca
     4981 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tctgaagagg
     5041 agtttacgtc cagccaagct taggatcaat tctcatgttt gacagcttat catcgataag
     5101 ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg
     5161 tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa
     5221 gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca
     5281 gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg
     5341 ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc
     5401 tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg
     5461 tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
     5521 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
     5581 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
     5641 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
     5701 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
     5761 gagcaggagc gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag
     5821 gaggaggggc aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag
     5881 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
     5941 gaggggcagg aggggcagga gsaggaggag gggcaggagg ggsaggaggg gcaggagcag
     6001 gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag
     6061 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag
     6121 gggcaggagc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     6181 caggaggggc aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
     6241 gggcaggagc aggaggggca ggaggggcag gagcaggagg ggsaggaggg gcaggagcag
     6301 gaggaggggs aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg
     6361 caggaggggs aggagsagga ggggsaggag gggsaggags aggaggggsa ggaggggcag
     6421 gagsaggagg aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag
     6481 gaggcagtgg aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag
     6541 gccgccgggg tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga
     6601 gaggtcgtgg acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg
     6661 ggtctccacc gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag
     6721 ccgattattt tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg
     6781 gagcgataga gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg
     6841 gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag
     6901 gaggttccaa cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga
     6961 gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag
     7021 gtagtaagac ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc
     7081 gtcttacacc attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg
     7141 gcccgctaag ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg
     7201 ctgaggtttt gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca
     7261 atatcagggt gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac
     7321 ctatggtgga aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg
     7381 atggagatga gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc
     7441 gtattaaaag ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt
     7501 gctgttggtg tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc
     7561 aattgccgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
     7621 ctcgaagagg ttcactagcg gatctcaatt gggcataccc atgttgtcac gtcactcagc
     7681 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
     7741 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
     7801 aggaaaagga caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg
     7861 atagcactcc cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca
     7921 tatgctaccc ggatacagat taggatagca tatactaccc agatatagat taggatagca
     7981 tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat taggatagca
     8041 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     8101 tatgctaccc agatatagat taggatagca tatgctaccc agatatagat taggatagca
     8161 tatgctatcc agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata
     8221 ctaccctaat ctctattagg atagcatatg ctacccggat acagattagg atagcatata
     8281 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     8341 ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
     8401 ctacccagat atagattagg atagcctatg ctacccagat atagattagg atagcatatg
     8461 ctatccagat atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca
     8521 gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg
     8581 taatttgtcc tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg
     8641 caggtattcc ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt
     8701 agcggggtta caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc
     8761 ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt
     8821 gtctttgttt atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga
     8881 caaccagtgg agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt
     8941 gtaacaacat ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta
     9001 tcatattgca ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc
     9061 agtctttacg gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt
     9121 cattcctaca ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc
     9181 acaatgccac cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt
     9241 gttttcgagc acctcacata caccttactg ttcacaactc agcagttatt ctattagcta
     9301 aacgaaggag aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat
     9361 cttcagccac tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat
     9421 gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt
     9481 actaacccta attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg
     9541 ttagtctgga tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca
     9601 agggggcctt ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac
     9661 acaggcccct ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg
     9721 tacatgtccc ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt
     9781 tgtgttgcag tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca
     9841 aatgtgcaca tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt
     9901 ccccccccgt gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag
     9961 ggattacatg cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt
    10021 ccggggcggg gatcgatcct ctagagtcga cgctagcgga tccgctgcat taatgaatcg
    10081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gg
//
