LOCUS       sequence-43120-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Radhakrishnan SK, Lee CS, Young P, Beskow A, Chan JY, Deshaies RJ
  TITLE     Transcription factor Nrf1 mediates the proteasome recovery pathway 
            after proteasome inhibition in mammalian cells.
  JOURNAL   Mol Cell. 2010 Apr 9;38(1):17-28.
  PUBMED    20385086
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-04-9"; volume: "38(1)"; pages: "17-28"
COMMENT     Sequence Label: Flag-NRF1-HA
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tccttctcta ggcgccggaa ttagatccgc caccatggac tacaaagacc atgacggtga
       61 ttataaagat catgatatcg attacaagga tgacgatgac aagagatctg cggccgctct
      121 ttctctgaag aaatacttaa cggaaggact tctccagttc accattctgc tgagtttgat
      181 tggggtacgg gtggacgtgg atacttacct gacctcacag cttcccccac tccgggagat
      241 catcctgggg cccagttctg cctatactca gacccagttc cacaacctga ggaatacctt
      301 ggatggctat ggtatccacc ccaagagcat agacctggac aattacttca ctgcccggcg
      361 gctcctcagt caggtgaggg ccctggacag gttccaggtg ccaaccactg aggtaaatgc
      421 ctggctggtt caccgagacc cagaggggtc tgtctctggc agtcagccca actcaggcct
      481 cgccctcgag agttccagtg gcctccaaga tgtgacaggc ccagacaacg gggtgcgaga
      541 aagcgaaacg gagcagggat tcggtgaaga tttggaggat ttgggggctg tagccccccc
      601 agtcagtgga gacttaacca aagaggacat agatctgatt gacatccttt ggcgacagga
      661 tattgatctg ggggctgggc gtgaggtttt tgactatagt caccgccaga aggagcagga
      721 tgtggag
//
