LOCUS       sequence-431238-        8807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homologous recombination donor for insertion of a HaloTag at the 
            N-terminus of the endogenous 53BP1 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Heyza JR, Mikhova M, Bahl A, Broadbent DG, Schmidt JC
  TITLE     Systematic analysis of the molecular and biophysical properties of 
            key DNA damage response factors.
  JOURNAL   Elife. 2023 Jun 21;12:e87086. doi: 10.7554/eLife.87086.
  PUBMED    37341699
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-21"; volume: "12:e87086. doi"; pages: " 
            10.7554/eLife.87086"
COMMENT     Sequence Label: Halo-53BP1 HRD
FEATURES             Location/Qualifiers
     source          1..8807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4060..4108
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4133..4152
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(4244..4353)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4377..4468
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4393..4410
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5471..5536
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5537..5570)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5577..5625
                     /note="synthetic polyadenylation signal"
     CDS             complement(5626..6225)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5709..5729)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6206..6225
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6232..6548)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6246..6381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    6555..6588
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6597..7487
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             7500..7520
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     polyA_signal    8297..8431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8334..8353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8388..8407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8460..8625
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtacccgta gtggctatgg
     3901 cagggcttgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttgggggtt
     3961 ggggtgggga aaaggaagaa acgcgggcgt attggtccca atggggtctc ggtggggtat
     4021 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg
     4081 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     4141 ttccgtgttt cagttagcct cccccatctc ccggtaccgc atgctatgca tcagctgcta
     4201 gcaccatggc tcgagatccc gggtgatcaa gtcttcgtcg agtgattgta aataaaatgt
     4261 aatttacagt atagtatttt aattaatata caaatgattt gataataatt cttatttaac
     4321 tataatatat tgtgttgggt tgaattaaag gtccgtatac tccggaatat taatagatca
     4381 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     4441 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
     4501 gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct acgtcgacga gctcactagt
     4561 cgcggccgct ttcgaatcta gagcctgcag tctcgacaag cttgtcgaga agtactagag
     4621 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     4681 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttcagc gaatggacga
     4741 tccgatattg tgtgacgtga cgggaaaggg ggagttcgcg gccggtggcg gcggtggcga
     4801 cagcggcgac ctagggatcg atctggaggg acttggggag cgtgcagaga cctctagctc
     4861 gagcgcgagg gacctcccgc cgggatgcct ggtactgttt ggagagaaat gggagtgcag
     4921 tgggctctga aggcctggat ttcgggtctg aagggatggc tgaccggagg gggcggggtg
     4981 gcaagcatgg cggcggtgac ggggaagggg ttggagggcc ctaaggaggg ggtggggaaa
     5041 cgttagctaa gaaggcttgg tggtcgtgag gaaagctgaa aggctgtggg gcctctggcg
     5101 aacagatttt agaaaacatt cttttggggg gaggtagagg cgccagaagg acgtggcggg
     5161 ggggatggaa aaaggggcgg ggagagaaga ggttaggaag gaggggaaag ggtgtctacg
     5221 agaaggtgat gaaaaatagg gagggatagt caggcttatt gtaaataacg aggaatggtg
     5281 acttggtagg catgatagga aagtgcttgc agcaattata aatataatat tctaattgga
     5341 ggagtcctga agtggtcccg tttcccagct cttgtacttc cgccccatta tttaggttta
     5401 gactgcgtga gcaaggctag taggttacac ggaactgatc ttttgttatt ccattccagg
     5461 ggagcagatg gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
     5521 ggatgacgat gacaagataa cttcgtatag catacattat acgaagttat aagcttcaca
     5581 caaaaaacca acacacagat gtaatgaaaa taaagatatt ttatttcagg caccgggctt
     5641 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     5701 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     5761 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     5821 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     5881 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     5941 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     6001 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     6061 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     6121 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     6181 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatgagct ttttgcaaaa
     6241 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6301 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6361 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     6421 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     6481 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     6541 tccacaccgg atccataact tcgtataatg tatgctatac gaagttatcc ggtaccatgg
     6601 cagaaatcgg tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca
     6661 tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc
     6721 cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca
     6781 ttgctccaga cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg
     6841 acgaccacgt ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc
     6901 tggtcattca cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc
     6961 gcgtcaaagg tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc
     7021 cagaatttgc ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga
     7081 tcatcgatca gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga
     7141 ctgaagtcga gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac
     7201 tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg
     7261 tcgaagaata catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca
     7321 ccccaggcgt tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact
     7381 gcaaggctgt ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga
     7441 tcggcagcga gatcgcgcgc tggctgtcga cgctcgagat ttccggcgag ccaaccactg
     7501 aggatctgta ctttcagagc gataacgcga tcgcttccgg taccatggac cctactggaa
     7561 gtcagttgga ttcagatttc tctcagcaag atactccttg cctgataatt gaagattctc
     7621 agcctgaaag ccaggttcta gaggatgatt ctggttctca cttcagtatg ctatctcgac
     7681 accttcctaa tctccagacg cacaaagaaa atcctgtgtt ggtgagtgat accttgtttg
     7741 aagaagattg agcagatttt tttttttaaa ccgaaaacca gcaaacataa gtatttagaa
     7801 gatcattacc ttctcaataa agttgattgg aaaaattgtt cacaaatagt aggtttcatt
     7861 ttgaactatt tcatatataa tggggatatt ttgtatgtgt attttctagg atgttgtgtc
     7921 caatcctgaa caaacagctg gagaagaacg aggagacggt aatagtgggt tcaatgaaca
     7981 tttgaaagaa aacaaggttg caggtgaggt gtttaaagct atctgtcccc tttgcatttt
     8041 ttgggtgctc ttgttggtgc tgggtggatt taaactttat gtggttgtgt cgaatgtatg
     8101 taaggaagcc tactgtggta tctgcgtagt agtcactcaa aagttgatgt gctagtgtca
     8161 ctgattagat tggtattgtc tgtattcctg attttgtatt aaagtttgtt ggtaaatgtc
     8221 tttcatgcta atatccagtt atttttttca gaccctgtgg attcttctaa cttggacaca
     8281 tgtggttcca tcagtcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8341 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8401 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga
     8461 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     8521 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     8581 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct
     8641 tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg
     8701 ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg
     8761 taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgg
//
