LOCUS       sequence-431240-        8065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of the human 20SCP proteasome assembly 
            chaperones.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
FEATURES             Location/Qualifiers
     source          1..8065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1906..1911,1912..1928,1929..1934))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2005..2038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2115..2206
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2843..2977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3133..3224
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      3265..3274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4302..4436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4592..4683
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5689..5823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5979..6070
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6650..6784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6940..7031
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7614..7748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  7900..8065
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     1741 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     1801 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     1861 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     1921 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     1981 accttgggca gcagcgaagt cgccataact tcgtatagca tacattatac gaagttatct
     2041 gtaactataa cggtcctaag gtagcgagtt taaacactag tatcgattcg cgacctactc
     2101 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     2161 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     2221 ataccgtccc accatcgggc gcggatccat gaatgccaga ggacttggat ctgagctaaa
     2281 ggacagtatt ccagttactg aactttcagc aagtggacct tttgaaagtc atgatcttct
     2341 tcggaaaggt ttttcttgtg tgaaaaatga acttttgcct agtcatcccc ttgaattatc
     2401 agaaaaaaat ttccagctca accaagataa aatgaatttt tccacactga gaaacattca
     2461 gggtctattt gctccgctaa aattacagat ggaattcaag gcagtgcagc aggttcagcg
     2521 tcttccattt ctttcaagct caaatctttc actggatgtt ttgaggggta atgatgagac
     2581 tattggattt gaggatattc ttaatgatcc atcacaaagc gaagtcatgg gagagccaca
     2641 cttgatggtg gaatataaac ttggtttact gtaataggcg gccgctttcg aatctagagc
     2701 ctgcagtctc gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     2761 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2821 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag
     3001 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     3061 aactatcata accatttaaa taaactggat acaattgcac gtttaaatcg acctactccg
     3121 gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat
     3181 tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat
     3241 accgtcccac catcgggcgc ggatccatgg cggccacgtt cttcggagag gtggtgaagg
     3301 cgccgtgccg agctgggact gaggacgaag aggaggagga ggaggggcgg agggagacgc
     3361 ccgaggacag ggaggtgcgt ctgcagctgg cgcggaagag ggaagtgcgg ctccttcgaa
     3421 gacaaacaaa aacatctttg gaagtttctt tgctagaaaa atatccgtgc tccaagttta
     3481 taattgctat aggaaataat gcagtagcat ttctgtcatc atttgttatg aattcaggag
     3541 tctgggagga agttggttgt gctaaactct ggaatgaatg gtgtagaaca acagacacta
     3601 cacatctgtc ctccacagag gctttttgtg tgttttatca tctaaaatcc aatccctcgg
     3661 tttttctctg tcagtgcagt tgctatgttg cagaagatca acagtatcag tggctggaaa
     3721 aggtttttgg ctcttgtcca aggaagaaca tgcagataac tattctcaca tgtcgacatg
     3781 ttaccgatta taaaacctca gaatccaccg gcagccttcc ttctcctttc ctgagagccc
     3841 taaaaacaca gaatttcaaa gactcggcgt gttgtccatt gctagaacaa ccgaatatag
     3901 tacacgacct tcctgcagca gttctaagct actgtcaagt atggaaaatc ccggcaattc
     3961 tgtacttgtg ttatactgat gtgatgaaat tagacctaat cacagtggaa gcttttaagc
     4021 ctatactttc taccagaagc ttgaagggtt tggttaagaa tattccccaa agcactgaga
     4081 tactaaagaa attgatgaca acaaatgaga ttcagagtaa catttataca tgataagcgg
     4141 ccgctttcga atctagagcc tgcagtctcg acaagcttgt cgagaagtac tagaggatca
     4201 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4261 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     4441 cactgcttga gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     4501 gctgccaccg ctgagcaata actatcataa ccatttaaat aaacctaatg atgcctgatg
     4561 tttaaatcga cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat
     4621 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
     4681 aatattccgg attattcata ccgtcccacc atcgggcgcg gatccatgtt cgttccctgc
     4741 ggggagtcgg cccccgacct tgccggcttc accctcctaa tgccagcagt atctgttgga
     4801 aatgttggcc agcttgcaat ggatctgatt atttctacac tgaatatgtc taagattggt
     4861 tacttctata ccgattgtct tgtgccaatg gttggaaaca atccatatgc gaccacagaa
     4921 ggaaattcaa cagaacttag cataaatgct gaagtgtatt cattgccttc aagaaagctg
     4981 gtggctctac agttaagatc catttttatt aagtataaat caaagccgtt ctgtgaaaaa
     5041 ctgctttcct gggtgaaaag cagtggctgt gccagagtca ttgttctttc aagcagtcat
     5101 tcatatcagc gtaatgatct gcagcttcgt agtactccct tccggtacct acttacacct
     5161 tccatgcaaa aaagtgttca aaataaaata aagagcctta actgggaaga aatggaaaaa
     5221 agccggtgca ttcctgaaat agatgattcc gagttttgta tccgcattcc gggaggaggt
     5281 atcacaaaaa cactctatga tgaaagctgt tctaaagaaa tccaaatggc agttctgctg
     5341 aaatttgttt cagaagggga caacatccca gatgcattag gtcttgttga gtatcttaat
     5401 gagtggcttc agatactcaa accacttagc gatgacccca cagtatctgc ctcacggtgg
     5461 aaaataccaa gttcttggag attactcttt ggcagtggtc ttccccctgc acttttctga
     5521 taagcggccg ctttcgaatc tagagcctgc agtctcgaca agcttgtcga gaagtactag
     5581 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     5641 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5701 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5761 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5821 atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga
     5881 gttggctgct gccaccgctg agcaataact atcataacca tttaaataaa cggttcacat
     5941 agcttagttt aaatcgacct actccggaat attaatagat catggagata attaaaatga
     6001 taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa
     6061 acctataaat attccggatt attcataccg tcccaccatc gggcgcggat ccatggaaga
     6121 cacgccgttg gtgatatcga agcagaagac ggaggtggtg tgcggggtcc ccacccaggt
     6181 ggtgtgtacg gccttcagca gtcacatcct ggtggtggtg acccagtttg ggaagatggg
     6241 caccctggtc tccctggagc ccagcagcgt ggccagtgac gtcagcaagc ctgtgctcac
     6301 cacaaaagtc cttctggggc aggatgagcc tctcatccat gtctttgcaa agaacctggt
     6361 agcgtttgtg tctcaagaag ctggaaacag agcagtcctc ctcgccgtgg ccgtgaagga
     6421 caaaagcatg gaggggctga aggcgctgag ggaggtgatc cgggtgtgcc aggtgtggtg
     6481 ataagcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta
     6541 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6601 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6781 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
     6841 agttggctgc tgccaccgct gagcaataac tatcataacc atttaaataa acactgacat
     6901 tgacttggtt taaatcgacc tactccggaa tattaataga tcatggagat aattaaaatg
     6961 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     7021 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga tccatggagg
     7081 ggctggttgt cgccgccggc ggggacgtct ccctgcacaa cttcagcgcg aggctgtggg
     7141 agcagctggt ccacttccac gtcatgcggc tgacggactc gctgttcctg tgggtggggg
     7201 ccacgccgca cctgcgcaac ctcgccgtgg ccatgtgcag ccgctacgac tccatccccg
     7261 tgtctacctc cctccttgga gacacttccg acacgacctc tactggcctt gcccagcgcc
     7321 tagccaggaa gaccaacaaa caggtgtttg tcagctataa ccttcagaac acagacagta
     7381 acttcgcatt acttgtagaa aacaggatca aggaagagat ggaggctttc cccgaaaagt
     7441 tctagtaagc ggccgctttc gaatctagag cctgcagtct cgacaagctt gtcgagaagt
     7501 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7561 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7741 tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa
     7801 gctgagttgg ctgctgccac cgctgagcaa taactatcat aaccatttaa ataaatctat
     7861 atctcaatcg gggttcctag ggtataccca tctaattgga accagataag tgaaatctag
     7921 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc
     7981 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag
     8041 ttcccaacta ttttgtccgc ccaca
//
