LOCUS       sequence-43125-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Ngo HB, Kaiser JT, Chan DC
  TITLE     The mitochondrial transcription and packaging factor Tfam imposes a 
            U-turn on mitochondrial DNA.
  JOURNAL   Nat Struct Mol Biol. 2011 Oct 30;18(11):1290-6. doi: 
            10.1038/nsmb.2159.
  PUBMED    22037171
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-10-30- 30"; volume: 
            "18"; issue: "11"; pages: "1290-6"
COMMENT     Sequence Label: Human_TFAM_NoMTS_pET28
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             175..192
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             196..228
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gccacgatgc gtccggcgta gaggancgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
      181 ccgcgcggca gccatatggc tagcatgact ggtggacagc aaatgggtcg cggatccatg
      241 tcatctgtct tggcaagttg tccaaagaaa cctgtaagtt cttaccttcg attttctaaa
      301 gaacaactac ccatatttaa agctcagaac ccagatgcaa aaactacaga actaattaga
      361 agaattgccc agcgttggag ggaacttcct gattcaaaga aaaaaatata tcaagatgct
      421 tatagggcgg agtggcaggt atataaagaa gagataagca gatttaaaga acagctaact
      481 ccaagtcaga ttatgtcttt ggaaaaagaa atcatggaca aacatttaaa aaggaaagct
      541 atgacaaaaa aaaaagagtt aacactgctt ggaaaaccaa aaagacctcg ttcagcttat
      601 aacgtttatg tagctgaaag attccaagaa gctaagggtg attcaccgca ggaaaagctg
      661 aagactgtaa aggaaaactg gaaaaatctg tctgactctg aaaaggaatt atatattcag
      721 catgctaaag aggacgaaac tcgttatcat aatgaaatga agtcttggga agaacaaatg
      781 attgaagttg gacgaaagga tcttctacgt cgcacaataa agaaacaacg aaaatatggt
      841 gctgaggagt gt
//
