LOCUS       sequence-431260-       11848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of human 20S CP beta-subunits with a 
            c-terminal twin-strep-tag on beta2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: 
            pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
FEATURES             Location/Qualifiers
     source          1..11848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3184..3318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4404..4538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4694..4785
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5636..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5926..6017
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    7045..7179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7382..7473
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8498..8632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8788..8879
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9832..9966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10122..10213
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             11109..11129
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             join(11136..11141,11142..11165,11166..11201,11202..11225)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    11397..11531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  11683..11848
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tgttgtcctc tacagccatg tattcggctc ctggcagaga cttggggatg
     2341 gaaccgcaca gagccgcggg ccctttgcag ctgcgatttt cgccctacgt tttcaacgga
     2401 ggtactatac tggcaattgc tggagaagat tttgcaattg ttgcttctga tactcgattg
     2461 agtgaagggt tttcaattca tacgcgggat agccccaaat gttacaaatt aacagacaaa
     2521 acagtcattg gatgcagcgg ttttcatgga gactgtctta cgctgacaaa gattattgaa
     2581 gcaagactaa agatgtataa gcattccaat aataaggcca tgactacggg ggcaattgct
     2641 gcaatgctgt ctacaatcct gtattcaagg cgcttctttc catactatgt ttacaacatc
     2701 atcggtggac ttgatgaaga aggaaagggg gctgtataca gctttgatcc agtagggtct
     2761 taccagagag actccttcaa ggctggaggc tcagcaagtg ccatgctaca gcccctgctt
     2821 gacaaccagg ttggttttaa gaacatgcag aatgtggagc atgttccgct gtccttggac
     2881 agagcgatgc ggctggtgaa agatgtcttc atttctgcgg ctgagagaga tgtgtacact
     2941 ggggacgcac tccggatctg catagtgacc aaagagggca tcagggagga aactgtttcc
     3001 ttaaggaagg actgataagc ggccgctttc gaatctagag cctgcagtct cgacaagctt
     3061 gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
     3121 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3181 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3301 tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc
     3361 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat aaccatttaa
     3421 ataaactgga tactattgca cgtttaaatc gacctactcc ggaatattaa tagatcatgg
     3481 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtwmcrr
     3541 wkykskmrka awwmaamskm ywamkwykmc grrytmwcyw gtcgcggccg ctttcgaatc
     3601 tagatcccgg tccgaagcgc gcggaattca tggagtacct catcggtatc caaggccccg
     3661 actatgttct tgtcgcctcc gaccgggtgg ccgccagcaa tattgtccag atgaaggacg
     3721 atcatgacaa gatgtttaag atgagtgaaa agatattact cctgtgtgtt ggagaggctg
     3781 gagacactgt acagtttgca gaatatattc agaaaaacgt gcaactttat aagatgcgaa
     3841 atggatatga attgtctccc acggcagcag ctaacttcac acgccgaaac ctggctgact
     3901 gtcttcggag tcggacccca tatcatgtga acctcctcct ggctggctat gatgagcatg
     3961 aagggccagc gctgtattac atggactacc tggcagcctt ggccaaggcc ccttttgcag
     4021 cgcacggcta tggtgccttc ctgactctca gtatcctcga ccgatactac acaccgacta
     4081 tctcacgtga gagggcagtg gaactcctta ggaaatgtct ggaggagctc cagaaacgct
     4141 tcatcctgaa tctgccaacc ttcagtgttc gaatcattga caaaaatggc atccatgacc
     4201 tggataacat ttccttcccc aaacagggct cctaatgagc ggccgctttc gaatctagag
     4261 cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     4321 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     4381 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     4441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4501 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa
     4561 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     4621 taactatcat aaccatttaa ataaacctaa tgatgcctga tgtttaaatc gacctactcc
     4681 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     4741 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     4801 taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca tgtctattat
     4861 gtcctataac ggaggggccg tcatggccat gaaggggaag aactgtgtgg ccatcgctgc
     4921 agacaggcgc ttcgggatcc aggcccagat ggtgaccacg gacttccaga agatctttcc
     4981 catgggtgac cggctgtaca tcggtctggc cgggctcgcc actgacgtcc agacagttgc
     5041 ccagcgcctc aagttccggc tgaacctgta tgagttgaag gaaggtcggc agatcaaacc
     5101 ttataccctc atgagcatgg tggccaacct cttgtatgag aaacggtttg gcccttacta
     5161 cactgagcca gtcattgccg ggttggaccc gaagaccttt aagcccttca tttgctctct
     5221 agacctcatc ggctgcccca tggtgactga tgactttgtg gtcagtggca cctgcgccga
     5281 acaaatgtac ggaatgtgtg agtccctctg ggagcccaac atggatccgg atcacctgtt
     5341 tgaaaccatc tcccaagcca tgctgaatgc tgtggaccgg gatgcagtgt caggcatggg
     5401 agtcattgtc cacatcatcg agaaggacaa aatcaccacc aggacactga aggcccgaat
     5461 ggactaatga gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcgagaa
     5521 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5581 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5761 tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg
     5821 aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccattt aaataaacgg
     5881 ttcacatagc ttagtttaaa tcgacctact ccggaatatt aatagatcat ggagataatt
     5941 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
     6001 taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatccc
     6061 ggtccgaagc gcgcggaatt catggaagcg tttttggggt cgcggtccgg actttgggcg
     6121 gggggtccgg ccccaggaca gttttaccgc attccgtcca ctcccgattc cttcatggat
     6181 ccggcgtctg cactttacag aggtccaatc acgcggaccc agaaccccat ggtgaccggg
     6241 acctcagtcc tcggcgttaa gttcgagggc ggagtggtga ttgccgcaga catgctggga
     6301 tcctacggct ccttggctcg tttccgcaac atctctcgca ttatgcgagt caacaacagt
     6361 accatgctgg gtgcctctgg cgactacgct gatttccagt atttgaagca agttctcggc
     6421 cagatggtga ttgatgagga gcttctggga gatggacaca gctatagtcc tagagctatt
     6481 cattcatggc tgaccagggc catgtacagc cggcgctcga agatgaaccc tttgtggaac
     6541 accatggtca tcggaggcta tgctgatgga gagagcttcc tcggttatgt ggacatgctt
     6601 ggtgtagcct atgaagcccc ttcgctggcc actggttatg gtgcatactt ggctcagcct
     6661 ctgctgcgag aagttctgga gaagcagcca gtgctaagcc agaccgaggc ccgcgactta
     6721 gtagaacgct gcatgcgagt gctgtactac cgagatgccc gttcttacaa ccggtttcaa
     6781 accgccactg tcaccgaaaa aggtgttgaa atagagggac cattgtctac agagaccaac
     6841 tgggatattg cccacatgat cagtggcttt gaatgataag cggccgcttt cgaatctaga
     6901 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     6961 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     7021 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7081 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7141 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctaga
     7201 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     7261 ataactatca taaccattta aataaacact gacattgact tggtttccta gggtataccc
     7321 atctaaggta gcgagtttaa acactagtat cgattcgcga cctactccgg aatattaata
     7381 gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     7441 cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc
     7501 atcgggcgcg gatcccggtc cgaagcgcgc ggaattcatg gcgcttgcga gcgtgttgga
     7561 gagaccgcta ccggtgaacc agcgcgggtt tttcggactt gggggtcgtg cagatctgct
     7621 ggatctaggt ccagggagtc tcagtgatgg tctgagcctg gccgcgccag gctggggtgt
     7681 cccagaagag ccaggaatcg aaatgcttca tggaacaacc accctggcct tcaagttccg
     7741 ccatggagtc atagttgcag ctgactccag ggctacagcg ggtgcttaca ttgcctccca
     7801 gacggtgaag aaggtgatag agatcaaccc atacctgcta ggcaccatgg ctgggggcgc
     7861 agcggattgc agcttctggg aacggctgtt ggctcggcaa tgtcgaatct atgagcttcg
     7921 aaataaggaa cgcatctctg tagcagctgc ctccaaactg cttgccaaca tggtgtatca
     7981 gtacaaaggc atggggctgt ccatgggcac catgatctgt ggctgggata agagaggccc
     8041 tggcctctac tacgtggaca gtgaagggaa ccggatttca ggggccacct tctctgtagg
     8101 ttctggctct gtgtatgcat atggggtcat ggatcggggc tattcctatg acctggaagt
     8161 ggagcaggcc tatgatctgg cccgtcgagc catctaccaa gccacctaca gagatgccta
     8221 ctcaggaggt gcagtcaacc tctaccacgt gcgggaggat ggctggatcc gagtctccag
     8281 tgacaatgtg gctgatctac atgagaagta tagtggctct accccctgat aagcggccgc
     8341 tttcgaatct agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat
     8401 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8461 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8521 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     8641 gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     8701 ccaccgctga gcaataacta tcataaccat ttaaataaac tggatactat tgcacgttta
     8761 aatcgaccta ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     8821 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     8881 ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa
     8941 ttcatggcgg ctaccttact agctgctcgg ggagccgggc cagcaccggc ttgggggccg
     9001 gaggcattca ctccagactg ggaaagccga gaagtttcca ctgggacgac tatcatggcc
     9061 gtgcagtttg acgggggcgt ggttctgggg gcggactcca gaacaaccac tgggtcctac
     9121 atcgccaatc gagtgactga caagctgaca cctattcacg accgcatttt ctgctgtcgc
     9181 tcaggctcag ctgctgatac ccaggcagta gctgatgctg tcacctacca gctcggtttc
     9241 cacagcattg aactgaatga gcctccactg gtccacacag cagccagcct ctttaaggag
     9301 atgtgttacc gataccggga agacctgatg gcgggaatca tcatcgcagg ctgggaccct
     9361 caagaaggag ggcaggggta ctcagtgcct atggggggta tgatggtaag gcagtccttt
     9421 gccattggag gctccgggag ctcctacatc tatggctatg ttgatgctac ctaccgggaa
     9481 ggcatgacca aggaagagtg tctgcaattc actgccaatg ctctcgcttt ggccatggag
     9541 cgggatggct ccagtggagg agtgatccgc ctggcagcca ttgcagagtc aggggtagag
     9601 cggcaagtac ttttgggaga ccagataccc aaattcgccg ttgccacttt accacccgcc
     9661 tgataagcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt cgagaagtac
     9721 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     9781 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     9841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9961 tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc gaaaggaagc
    10021 tgagttggct gctgccaccg ctgagcaata actatcataa ccatttaaat aaacctaatg
    10081 atgcctgatg tttaaatcga cctactccgg aatattaata gatcatggag ataattaaaa
    10141 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
    10201 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatcccggtc
    10261 cgaagcgcgc ggaattcatg gcggctgtgt cggtgtatgc tccaccagtt ggaggcttct
    10321 cttttgataa ctgccgcagg aatgccgtct tggaagccga ttttgcaaag aggggataca
    10381 agcttccaaa ggtccggaaa actggcacga ccatcgctgg ggtggtctat aaggatggca
    10441 tagttcttgg agcagataca agagcaactg aagggatggt tgttgctgac aagaactgtt
    10501 caaaaataca cttcatatct cctaatattt attgttgtgg tgctgggaca gctgcagaca
    10561 cagacatgac aacccagctc atttcttcca acctggagct ccactccctc tccactggcc
    10621 gtcttcccag agttgtgaca gccaatcgga tgctgaagca gatgcttttc aggtatcaag
    10681 gttacattgg tgcagcccta gttttagggg gagtagatgt tactggacct cacctctaca
    10741 gcatctatcc tcatggatca actgataagt tgccttatgt caccatgggt tctggctcct
    10801 tggcagcaat ggctgtattt gaagataagt ttaggccaga catggaggag gaggaagcga
    10861 agaatctggt gagcgaagcc atcgcagctg gcatcttcaa cgacctgggc tccggaagca
    10921 acattgacct ctgcgtcatc agcaagaaca agctggattt tctccgccca tacacagtgc
    10981 ccaacaagaa ggggaccagg cttggccggt acaggtgtga gaaagggact actgcagtcc
    11041 tcactgagaa aatcactcct ctggagattg aggtgctgga agaaacagtc caaacaatgg
    11101 acacttccga ggatctgtac tttcagtctg tcgacagtgc ttggagccat ccacagtttg
    11161 agaaaggtgg aggctctggc gggggtagcg ggggcagtgc ctggtctcac ccacaattcg
    11221 aaaagtgata agcggccgct ttcgaatcta gagcctgcag tctcgacaag cttgtcgaga
    11281 agtactagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
    11341 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    11401 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11461 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11521 atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa agcccgaaag
    11581 gaagctgagt tggctgctgc caccgctgag caataactat cataaccatt taaataaacg
    11641 gttcacatag cttagtttcc tagggtatac ccatctaatt ggaaccagat aagtgaaatc
    11701 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
    11761 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
    11821 cagttcccaa ctattttgtc cgcccaca
//
