LOCUS       sequence-431287-        8304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to make a lenti virus for stable expression of the G protein 
            beta 5 subunit in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE,
            Riggi M, Iwasa JH, Willardson BM, Shen PS
  TITLE     Visualizing the chaperone-mediated folding trajectory of the G 
            protein beta5 beta-propeller.
  JOURNAL   Mol Cell. 2023 Nov 2;83(21):3852-3868.e6. doi: 
            10.1016/j.molcel.2023.09.032. Epub 2023 Oct 17.
  PUBMED    37852256
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.09.032"; journalName: "Mol Cell"; date: 
            "2023-11-2- 2"; volume: "83"; issue: "21"; pages: "3852-3868.e6"
COMMENT     Sequence Label: pLenti-Puro 2xStrep-HPC4-Gb5
FEATURES             Location/Qualifiers
     source          1..8304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2119..2322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2273..2293
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2324..2342
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             2431..2454
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2494..2517
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3715..3750
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     primer_bind     complement(3781..3798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(3810..3830)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3815..4144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3995..4130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4154..4753
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4154..4173)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4650..4670
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4825..5058
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5130..5251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5167..5186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5221..5240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5291..5426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5446..5465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5447..5465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5475..5492)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5475..5491)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5484..5506)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5633..6088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5720..5739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5930..5951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6114..6218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6219..6287,6288..7079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6437..6456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7739..7758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7992..8009
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8126..8147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8162..8167,8168..8185,8186..8192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8200..8216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8205..8227
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8224..8240
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8224..8240
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8259..8279
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8261..8279
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1861 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1921 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1981 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2041 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2101 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2161 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2221 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2281 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2341 gatctcccta tcagtgatag agatcgtcga ctagtggatc cagtgtggtg gaattctgca
     2401 gatatccagc acagtggtgc aggatccatg tggagccacc cgcagttcga aaaaggaggt
     2461 ggttctggtg gaggttctgg tggttcatca gcttggagcc acccgcagtt cgaaaaagct
     2521 gctatgtgtg atcagacctt tctcgttaat gtatttggct catgtgacaa atgtttcaaa
     2581 caacgagctc tgagaccagt tttcaagaag tctcaacaac tcagctactg ttcaacatgt
     2641 gcagaaatta tggcaaccga ggggctgcac gagaacgaga cgctggcgtc gctgaagagc
     2701 gaggccgaga gcctcaaggg caagctggag gaggagcgag ccaagctgca cgatgtggag
     2761 ctgcaccagg tggcggagcg ggtggaggcc ctggggcagt ttgtcatgaa gaccagaagg
     2821 accctcaaag gccacgggaa caaagtcctg tgcatggact ggtgcaaaga taagaggagg
     2881 atcgtgagct cgtcacagga tgggaaggtg atcgtgtggg attccttcac cacaaacaag
     2941 gagcacgcgg tcaccatgcc ctgcacgtgg gtgatggcat gtgcttatgc cccatcggga
     3001 tgtgccattg cttgtggtgg tttggataat aagtgttctg tgtacccctt gacgtttgac
     3061 aaaaatgaaa acatggctgc caaaaagaag tctgttgcta tgcacaccaa ctacctgtcg
     3121 gcctgcagct tcaccaactc tgacatgcag atcctgacag cgagcggcga tggcacatgt
     3181 gccctgtggg acgtggagag cgggcagctg ctgcagagct tccacggaca tggggctgac
     3241 gtcctctgct tggacctggc cccctcagaa actggaaaca ccttcgtgtc tgggggatgt
     3301 gacaagaaag ccatggtgtg ggacatgcgc tccggccagt gcgtgcaggc ctttgaaaca
     3361 catgaatctg acatcaacag tgtccggtac taccccagtg gagatgcctt tgcttcaggg
     3421 tcagatgacg ctacgtgtcg cctctatgac ctgcgggcag atagggaggt tgccatctat
     3481 tccaaagaaa gcatcatatt tggagcatcc agcgtggact tctccctcag tggtcgcctg
     3541 ctgtttgctg gatacaatga ttacactatc aacgtctggg atgttctcaa agggtcccgg
     3601 gtctccatcc tgtttggaca tgaaaaccgc gttagcactc tacgagtttc ccccgatggg
     3661 actgctttct gctctggatc atgggatcat accctcagag tctgggccgg tggagaggac
     3721 caggtggacc ccaggctgat cgacggcaag taagaattca aggttaaacc tgctgatcag
     3781 cctcgactgt gccttctagt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3961 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4021 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4081 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4141 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4201 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4261 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4321 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4381 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4441 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4501 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4561 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4621 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4681 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4741 gcccggtgcc tgaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4801 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4861 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4921 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4981 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5041 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5101 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5161 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5221 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5341 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5401 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5461 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5521 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5581 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5641 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5701 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5761 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5821 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5881 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5941 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6001 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6061 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6181 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6241 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6301 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6361 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6421 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6481 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6541 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6601 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6661 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6721 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6781 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6841 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6901 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6961 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7021 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7081 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7921 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7981 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8041 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8101 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8161 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8221 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8281 aacaaaagct ggagctgcaa gctt
//
