LOCUS       sequence-431374-       10255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-JAG1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4094..4129
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4139..4162
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5822..5866
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5867..5893
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6033..6050)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6039..6150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6268..6288)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6273..6602
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6453..6588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6515..6534
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6650..6697
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6716..7315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6716..6735)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7212..7232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7445..7566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7482..7501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7536..7555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7619..8410,8411..8479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8242..8261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8480..8571)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8613..8632
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8638..8657
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8743..9331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9232..9251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9485..9502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9600..9617
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9601..9617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10073..10089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg cccaatttga gctggaaatc ctgagcatgc agaacgtgaa
     1021 cggcgagctg cagaatggca attgctgtgg cggagccaga aatcccggcg acagaaagtg
     1081 caccagagat gagtgcgaca cctacttcaa agtgtgcctg aaagaatacc agagccgcgt
     1141 gacagccggc ggaccttgtt cttttggcag cggctctaca cctgtgatcg gcggcaacac
     1201 cttcaacctg aaggccagca gaggcaacga ccggaacaga atcgtgctgc ccttcagctt
     1261 cgcctggcct agaagctaca ccctgctggt ggaagcctgg gacagcagca atgataccgt
     1321 gcagcccgac agcatcatcg agaaggcctc tcacagcggc atgatcaacc ccagcagaca
     1381 gtggcagacc ctgaagcaga ataccggcgt ggcccacttc gagtaccaga tcagagtgac
     1441 ctgcgacgac tactactacg gcttcggctg caacaagttc tgcagacccc gggacgattt
     1501 cttcggccac tatgcctgcg accagaacgg caacaagacc tgcatggaag gctggatggg
     1561 ccccgagtgc aatagagcca tctgcagaca gggctgtagc cccaagcacg gcagctgtaa
     1621 actgcccggc gattgcagat gccagtatgg ctggcagggc ctgtactgcg acaagtgcat
     1681 tcctcatcct ggctgcgtgc acggaatctg caatgagcct tggcagtgcc tgtgcgagac
     1741 aaactgggga ggccagctgt gcgacaagga cctgaattac tgcggcaccc accagccttg
     1801 cctgaatggc ggcacatgta gcaacacagg ccccgacaag taccagtgct cttgccctga
     1861 gggctactct ggccccaatt gcgagattgc cgaacacgcc tgtctgagcg acccctgcca
     1921 caatagaggc agctgcaaag agacaagcct gggcttcgag tgcgagtgtt ctcctggatg
     1981 gacaggccct acctgcagca ccaacatcga cgactgcagc cctaacaact gcagccacgg
     2041 cggaacctgt caggacctgg tcaacggctt taagtgcgtg tgccctcctc agtggaccgg
     2101 caaaacatgc cagctggacg ccaacgagtg cgaagccaag ccttgtgtga acgccaagag
     2161 ctgcaagaac ctgatcgcca gctactactg cgactgcctg ccaggatgga tgggacagaa
     2221 ctgcgacatc aacatcaacg attgcctggg ccagtgccag aacgacgcca gctgtagaga
     2281 tctggtcaat ggctaccggt gcatctgccc acctggatat gcaggcgatc actgcgagcg
     2341 ggacattgac gagtgtgcca gcaatccctg tctgaacggc ggccactgtc agaacgagat
     2401 caaccggttc cagtgtctgt gccccaccgg ctttagcggc aatctgtgtc agctggacat
     2461 cgactattgc gagcccaatc catgccagaa tggcgcccag tgctacaaca gagccagcga
     2521 ctacttctgc aagtgccccg aggattacga gggcaagaac tgctcccacc tgaaggacca
     2581 ctgccggacc acaccttgcg aagtgatcga tagctgcacc gtggctatgg ccagcaacga
     2641 tacaccagaa ggcgtgcggt acatcagcag caacgtgtgt ggacctcacg gcaagtgcaa
     2701 gtctcagagc ggcggcaagt tcacctgtga ctgtaacaag ggcttcaccg gcacctactg
     2761 ccacgagaac atcaatgact gcgagagcaa cccctgcaga aacggcggca cttgtatcga
     2821 cggcgtgaac agctacaagt gtatctgcag cgacggatgg gagggcgcct actgcgagac
     2881 taatatcaac gactgctctc agaacccttg ccacaacggc gggacctgca gagatctcgt
     2941 gaacgacttc tactgcgatt gcaagaacgg ctggaagggc aagacctgtc acagcagaga
     3001 tagccagtgc gacgaggcca cctgtaacaa tggcggaact tgctacgatg agggcgacgc
     3061 cttcaagtgc atgtgtcctg gtggatggga aggcaccaca tgcaatatcg cccggaacag
     3121 cagctgtctg cctaatccat gtcataacgg tggcacctgt gtcgtgaatg gcgagagctt
     3181 tacctgcgtg tgcaaagaag gctgggaggg acctatctgc gcccagaaca ccaacgactg
     3241 tagcccacat ccttgctaca actccggcac atgcgtggac ggcgacaact ggtacagatg
     3301 cgaatgcgcc cctggcttcg ccggacctga ttgccggatc aatatcaatg agtgtcagag
     3361 cagcccttgc gccttcggcg ctacttgtgt ggatgagatc aacggctacc gctgcgtgtg
     3421 tccaccaggc catagcggag ccaagtgtca agaagtgtcc ggcagacctt gcatcaccat
     3481 gggcagcgtt atccccgatg gcgccaagtg ggacgacgat tgcaatacct gccagtgcct
     3541 caacggcaga atcgcctgct ccaaagtttg gtgcggcccc agaccttgtc tgctgcacaa
     3601 gggacacagc gagtgtccta gcggccagag ctgtatcccc atcctggacg atcagtgctt
     3661 cgtgcaccct tgtacaggcg tgggcgagtg tagaagcagc tctctgcagc ccgtgaaaac
     3721 aaagtgtacc agcgatagct actaccagga caactgcgcc aacatcacct tcacgttcaa
     3781 caaagaaatg atgagccctg gcctgaccac cgagcacatc tgtagcgagc tgagaaacct
     3841 gaacatcctg aagaacgtgt ccgccgagta cagcatctat atcgcctgcg agccttctcc
     3901 tagcgccaac aacgaaatcc acgtggccat cagcgccgag gacatcagag atgacggcaa
     3961 ccccatcaaa gagatcaccg acaagatcat cgacctggtg tccaagcgcg acggcaactc
     4021 ttctctgatt gctgccgtcg ctgaagtgcg ggtccagaga aggcccctga agaatagaac
     4081 cgatggcgcg ccggaagatc aagttgatcc aagattgatt gatggcaagg gcggatctct
     4141 ggaggtgctg ttccagggac caggcggaag cggcaatccc ggcggcgatc tggcccccca
     4201 gatgctgcgc gagctgcagg agaccaatgc cgccctgcag gacgtgcgcg agctgctgcg
     4261 ccaccgcgtg aaggagatca ccttcctgaa gaacaccgtg atggagtgcg acgcctgcgg
     4321 tatgcagccc tctagaggct ccggctccat catcccagtt gaggaggaga acccggactt
     4381 ctggaaccgc gaggcagccg aggccctggg tgccgccaag aagctgcagc ctgcacagac
     4441 agccgccaag aacctcatca tcttcctggg cgatgggatg ggggtgtcta cggtgacagc
     4501 tgccaggatc ctaaaagggc agaagaagga caaactgggg cctgagatac ccctggccat
     4561 ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat gtagacaaac atgtgccaga
     4621 cagtggagcc acagccacgg cctacctgtg cggggtcaag ggcaacttcc agaccattgg
     4681 cttgagtgca gccgcccgct ttaaccagtg caacacgaca cgcggcaacg aggtcatctc
     4741 cgtgatgaat cgggccaaga aagcagggaa gtcagtggga gtggtaacca ccacacgagt
     4801 gcagcacgcc tcgccagccg gcacctacgc ccacacggtg aaccgcaact ggtactcgga
     4861 cgccgacgtg cctgcctcgg cccgccagga ggggtgccag gacatcgcta cgcagctcat
     4921 ctccaacatg gacattgacg tgatcctagg tggaggccga aagtacatgt ttcgcatggg
     4981 aaccccagac cctgagtacc cagatgacta cagccaaggt gggaccaggc tggacgggaa
     5041 gaatctggtg caggaatggc tggcgaagcg ccagggtgcc cggtatgtgt ggaaccgcac
     5101 tgagctcatg caggcttccc tggacccgtc tgtgacccat ctcatgggtc tctttgagcc
     5161 tggagacatg aaatacgaga tccaccgaga ctccacactg gacccctccc tgatggagat
     5221 gacagaggct gccctgcgcc tgctgagcag gaacccccgc ggcttcttcc tcttcgtgga
     5281 gggtggtcgc atcgaccatg gtcatcatga aagcagggct taccgggcac tgactgagac
     5341 gatcatgttc gacgacgcca ttgagagggc gggccagctc accagcgagg aggacacgct
     5401 gagcctcgtc actgccgacc actcccacgt cttctccttc ggaggctacc ccctgcgagg
     5461 gagctccatc ttcgggctgg cccctggcaa ggcccgggac aggaaggcct acacggtcct
     5521 cctatacgga aacggtccag gctatgtgct caaggacggc gcccggccgg atgttaccga
     5581 gagcgagagc gggagccccg agtatcggca gcagtcagca gtgcccctgg acgaagagac
     5641 ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg caggcgcacc tggttcacgg
     5701 cgtgcaggag cagaccttca tagcgcacgt catggccttc gccgcctgcc tggagcccta
     5761 caccgcctgc gacctggcgc cccccgccgg caccaccgac gccgcgcacc cgggtggcgg
     5821 aggactgaac gacatcttcg aggctcagaa aatcgaatgg cacgaacatc accatcacca
     5881 tcaccatcac cattaaggcc ggcctgtagt cactggcaca acgcgttacc cggtaagcca
     5941 atcgggtata cacggtcgtc atactgcaga cagggttctt ctactttgca agatagtctt
     6001 gagtagtaaa ataaatagat agagaaaatc agcctcgact gtgccttcta gttgccagcc
     6061 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6121 cctttcctaa taaaatgagg aaattgcatc acaacactca accctatctc ggtctattct
     6181 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6241 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6361 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6421 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6481 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6541 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6601 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6661 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatgac
     6721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6901 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6961 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7141 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7201 atccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacacgt
     7321 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7381 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7441 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7561 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcatt
     7621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7741 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
     7801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7921 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8461 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
     8581 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     8641 gctcataaca ccccttgctc atgaccaaaa tcccttaacg tgagttacgc gcgcgtcgtt
     8701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8881 aaatactgtt cttctagtgt agccgtagtt agcccaccac ttcaagaact ctgtagcacc
     8941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9361 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9421 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9481 gcgcagcgag tcagtgagcg aggaagcgga aggcgagagt agggaactgc caggcatcaa
     9541 actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc tttctgtgtt
     9601 gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa cgagtaatcg
     9661 ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg ggagtttagg
     9721 gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata tatgagaatt
     9781 atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt tgctttttcg
     9841 attgatgaac acctataatt aaactattca tctattattt atgatttttt gtatatacaa
     9901 tatttctagt ttgttaaaga gaattaagaa aataaatctc gaaaataata aagggaaaat
     9961 cagtttttga tatcaaaatt atacatgtca acgataatac aaaatataat acaaactata
    10021 agatgttatc agtatttatt atcatttaga ataaattttg tgtcgccctt aattgtgagc
    10081 ggataacaat tacgagcttc atgcacagtg gcgttgactc aatattggcc attagccata
    10141 ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
    10201 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgtt
//
