LOCUS       sequence-431375-       12304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH1(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..12304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6143..6178
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6188..6211
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7871..7915
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7916..7942
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8082..8099)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8088..8199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8317..8337)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8322..8651
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8502..8637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8564..8583
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8699..8746
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8765..9364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8765..8784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9261..9281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9494..9615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9531..9550)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9585..9604
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9668..10459,10460..10528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10291..10310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10529..10620)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10662..10681
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10687..10706
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10792..11380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11281..11300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11534..11551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     11649..11666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11650..11666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12122..12138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgcaagagg acctagatgt tctcaacctg gcgagacatg
     1021 cctgaacggc ggaaagtgtg aagccgccaa tggaaccgaa gcctgtgtgt gtggcggagc
     1081 ctttgtgggc cctagatgtc aagaccccaa tccttgtctg agcacccctt gcaagaatgc
     1141 cggcacctgt cacgtggtgg atagaagagg cgtggccgat tacgcctgta gctgtgccct
     1201 tggctttagc ggccctctgt gtctgacacc tctggacaac gcctgcctga caaacccctg
     1261 tagaaacggc ggcacatgcg atctgctgac cctgaccgag tacaagtgca gatgtcctcc
     1321 tggatggtcc ggcaagagct gtcagcaagc cgatccttgt gccagcaatc cttgcgccaa
     1381 tggcggccag tgtctgcctt ttgaggccag ctacatctgc cactgtcctc caagctttca
     1441 cggccctacc tgcagacagg atgtgaacga gtgtggccag aaacctggcc tgtgtagaca
     1501 tggcggaacc tgccacaacg aagtgggcag ctacagatgc gtgtgcagag ccactcacac
     1561 cggacctaat tgcgagaggc cttacgtgcc atgcagccca tcaccttgtc agaacggcgg
     1621 gacttgtaga cctaccggcg acgtgacaca cgagtgtgct tgtctgcctg gcttcaccgg
     1681 ccagaactgc gaggaaaaca tcgacgactg ccccggcaac aactgcaaaa atggcggagc
     1741 atgtgtggac ggcgtgaaca cctacaactg tagatgccct ccagagtgga caggccagta
     1801 ctgcacagag gatgtggatg agtgccagct gatgcccaat gcctgccaga atggcggcac
     1861 ttgccataat acccacggcg gctacaactg cgtgtgtgtg aatggatgga ccggcgagga
     1921 ctgctccgag aacatcgatg attgtgcctc cgccgcctgt ttccacggcg ccacatgtca
     1981 tgatagagtg gccagcttct actgcgagtg ccctcacgga agaacaggcc tgctgtgtca
     2041 cctgaacgac gcctgcatca gcaatccctg caacgagggc agcaactgcg acaccaatcc
     2101 tgtgaacggc aaggccatct gtacatgccc tagcggctat acaggacctg cctgtagtca
     2161 ggacgtggac gaatgttctc tgggcgccaa tccatgcgag cacgccggca aatgtatcaa
     2221 caccctgggc agcttcgagt gccagtgcct gcaaggatat acaggcccca gatgcgagat
     2281 cgatgtgaat gaatgcgtca gcaacccttg ccagaacgat gccacctgtc tggaccagat
     2341 cggcgagttc cagtgcatct gtatgcctgg ctatgagggc gtgcactgcg aagtgaatac
     2401 cgacgaatgc gccagctctc cctgcctgca caatggcaga tgcctggaca agatcaatga
     2461 gtttcagtgc gagtgtccca ccggctttac aggccacctg tgccagtatg atgtcgacga
     2521 gtgcgcctcc acaccttgca aaaacggcgc caagtgcctg gacggcccca atacctacac
     2581 ttgtgtgtgc accgagggct acaccggaac acactgcgag gtggacattg atgagtgcga
     2641 ccccgatcca tgtcactacg gcagctgtaa agacggcgtg gcaaccttca catgcctgtg
     2701 cagacctggc tatactggcc accactgcga gactaacatc aacgagtgta gcagccagcc
     2761 atgcaggcac ggtggaacct gtcaggacag agacaatgcc tacctgtgct tctgcctgaa
     2821 gggcaccacc gggcctaact gtgaaatcaa cctggacgac tgcgcaagca gcccttgcga
     2881 ttctggcact tgtctggata agatcgacgg ctatgagtgt gcctgcgagc ctggatacac
     2941 cggcagcatg tgcaacatca atatcgatga atgcgctggc aacccatgtc acaatggcgg
     3001 gacctgtgaa gatggcatca acggcttcac ctgtcggtgc cctgagggct accatgatcc
     3061 tacctgtctg tccgaagtga acgaatgcaa tagcaacccc tgcgtgcacg gcgcctgcag
     3121 agatagcctg aatggctaca agtgcgactg cgatccaggc tggtccggga ccaactgcga
     3181 tatcaacaac aacgaatgcg agtctaaccc ttgcgtcaac ggtggcactt gcaaggacat
     3241 gaccagcggc tacgtgtgca cctgtagaga gggcttctct ggccccaact gccagaccaa
     3301 tattaacgaa tgtgcttcta acccctgcct gaaccagggc acctgtatcg atgatgtggc
     3361 cggctataag tgcaactgtc tgctgcctta caccggggcc acatgtgaag tggttctggc
     3421 cccttgtgca ccctctccat gtcgtaatgg cggcgaatgc agacagagcg aggactacga
     3481 gagcttcagc tgtgtgtgcc caacaggctg gcagggacag acatgcgaag tggatatcaa
     3541 tgaatgtgtg ctgagcccct gcaggcatgg cgccagctgt cagaatactc atggcggcta
     3601 tagatgccac tgccaggccg gctacagcgg cagaaattgc gaaaccgata tcgacgattg
     3661 ccggcctaat ccatgccata acggcggctc ttgcaccgac ggcatcaaca ccgccttttg
     3721 cgattgcctg cctggattca gaggcacctt ctgcgaagag gacattaacg agtgtgcctc
     3781 cgatccttgc cggaatggcg ccaattgcac cgattgcgtg gacagctaca cctgtacctg
     3841 tcctgccggc ttttccggca tccactgcga aaacaacacc cctgactgca ccgagtccag
     3901 ctgcttcaat ggtggcacct gtgtggatgg gattaactcc tttacatgtc tgtgcccacc
     3961 tgggtttacc ggcagctact gtcagcacga tgtcaatgag tgtgactccc agccttgtct
     4021 gcacggcggt acatgtcaag atggctgcgg ctcctaccgg tgtacatgtc ctcaaggcta
     4081 cacagggccc aactgtcaga acctggtgca ttggtgcgac agcagcccat gcaagaatgg
     4141 cggaaagtgc tggcagaccc acacacagta tagatgcgaa tgcccttctg gctggaccgg
     4201 cctgtattgt gatgtgcctt ctgtgtcttg cgaggtcgcc gctcaaagac agggtgtcga
     4261 tgtggctaga ctgtgtcaac acggtggact gtgcgtggac gctggcaata cccatcattg
     4321 cagatgccag gctgggtaca caggcagcta ttgcgaggat ctggtggacg agtgctcccc
     4381 atctccatgc caaaatggcg ctacctgtac cgactacctc ggcggataca gctgcaaatg
     4441 tgtggccgga taccatggcg tgaactgcag cgaagagatt gacgagtgcc tgtctcaccc
     4501 ctgccaaaac ggtggtacat gcctggatct gcccaacaca tacaagtgct cttgccctag
     4561 aggcacccag ggtgtccatt gcgaaatcaa tgttgacgac tgcaaccctc ctgtggaccc
     4621 cgtgtccaga tctcccaagt gcttcaacaa tgggacctgc gtggaccaag tcggcggcta
     4681 ttcttgtact tgccctcctg gcttcgtggg cgagagatgt gaaggcgacg tcaacgaatg
     4741 tttgtctaat ccctgtgacg ccaggggcac ccagaattgc gtgcagagag tgaacgactt
     4801 ccactgtgaa tgcagagccg gacacactgg gagaagatgc gagtccgtga tcaatggctg
     4861 caagggcaaa ccatgtaaaa acggcggaac ttgcgccgtg gcctctaata ccgctagagg
     4921 cttcatctgc aagtgtcccg ccggatttga gggcgccact tgcgaaaatg atgccagaac
     4981 ctgcggcagc ctgagatgcc ttaatggtgg aacatgcatc agcggcccta gaagccctac
     5041 ttgcctgtgt ctgggccctt tcacaggccc cgagtgtcag tttcctgcca gttctccttg
     5101 tctcggcgga aacccctgct acaatcaggg aacatgcgag cctaccagcg agagcccctt
     5161 ctacagatgt ctgtgtcctg ccaagttcaa cggactgctg tgccacatcc tggattacag
     5221 ctttggaggc ggcgctggca gagacatccc tccacctctt attgaggaag cctgcgaact
     5281 gcccgagtgc caagaggatg ctggcaacaa agtgtgcagc ctgcagtgca acaatcacgc
     5341 ctgtggctgg gatggcggag actgctccct gaacttcaac gacccctgga agaactgcac
     5401 ccagtctctg cagtgctgga agtactttag cgacggccac tgcgacagcc agtgtaattc
     5461 tgccggctgc ctgttcgacg gcttcgattg tcagcgagct gagggccagt gcaatcccct
     5521 gtacgaccag tattgcaagg atcacttctc cgatggccac tgtgaccagg gctgtaatag
     5581 cgccgaatgc gaatgggatg gcctggattg tgccgagcac gtgccagaaa gactggctgc
     5641 tggaacactg gtggtggtcg tgctgatgcc tcctgagcag ctgagaaaca gcagcttcca
     5701 cttcctgcgc gagctgtcca gagtgctgca caccaatgtg gtgttcaaga gagatgccca
     5761 cggccagcag atgatcttcc catattacgg cagagaagag gaactgcgga agcaccctat
     5821 caagagagct gctgaaggct gggctgctcc tgatgctctg ctgggacaag tgaaagcctc
     5881 tctgcttcct ggcggaagcg aaggcggaag gcggagaaga gaactcgatc ctatggatgt
     5941 gcggggcagc atcgtgtacc tggaaatcga caacagacag tgcgtgcagg cctccagcca
     6001 atgttttcag tctgctaccg acgtggccgc ctttctggga gcacttgctt ccctgggatc
     6061 tctgaacatc ccctacaaga tcgaggccgt gcagtccgag acagtggaac ctcctcctcc
     6121 tgctcaactg catggcgcgc cggaagatca agttgatcca agattgattg atggcaaggg
     6181 cggatctctg gaggtgctgt tccagggacc aggcggaagc ggcaatcccg gcggcgatct
     6241 ggccccccag atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga
     6301 gctgctgcgc caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga
     6361 cgcctgcggt atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa
     6421 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     6481 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     6541 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     6601 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     6661 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     6721 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     6781 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     6841 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     6901 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     6961 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     7021 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     7081 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     7141 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     7201 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     7261 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     7321 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     7381 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     7441 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     7501 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     7561 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     7621 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     7681 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     7741 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     7801 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     7861 gggtggcgga ggactgaacg acatcttcga ggctcagaaa atcgaatggc acgaacatca
     7921 ccatcaccat caccatcacc attaaggccg gcctgtagtc actggcacaa cgcgttaccc
     7981 ggtaagccaa tcgggtatac acggtcgtca tactgcagac agggttcttc tactttgcaa
     8041 gatagtcttg agtagtaaaa taaatagata gagaaaatca gcctcgactg tgccttctag
     8101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8161 tcccactgtc ctttcctaat aaaatgagga aattgcatca caacactcaa ccctatctcg
     8221 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8281 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8401 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8461 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8581 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8641 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     8701 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8761 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8821 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8881 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8941 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     9001 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9061 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9121 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9181 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9241 cctggagaca tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9301 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9361 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     9421 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     9481 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9541 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9601 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     9661 atggtcatta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9781 ctggccccag cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag
     9841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9961 tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10501 gggcgacacg gaaatgttga atactcatat tcttcctttt tcaatattat tgaagcattt
    10561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10621 taggggtcag tgttacaacc aattaaccaa ttctgaacat tatcgcgagc ccatttatac
    10681 ctgaatatgg ctcataacac cccttgctca tgaccaaaat cccttaacgt gagttacgcg
    10741 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10801 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10861 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10921 gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact tcaagaactc
    10981 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    11041 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11101 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11161 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11221 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11281 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11341 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11401 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11461 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    11521 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta gggaactgcc
    11581 aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc tacaaactct
    11641 ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg ttctgataac
    11701 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatggggg
    11761 gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt tgcttgatat
    11821 atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat aagatacgtt
    11881 gctttttcga ttgatgaaca cctataatta aactattcat ctattattta tgattttttg
    11941 tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg aaaataataa
    12001 agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca aaatataata
    12061 caaactataa gatgttatca gtatttatta tcatttagaa taaattttgt gtcgccctta
    12121 attgtgagcg gataacaatt acgagcttca tgcacagtgg cgttgactca atattggcca
    12181 ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat
    12241 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
    12301 tgtt
//
