LOCUS       sequence-431419-        9652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for transgenic flies; gypsy insulator sequences; white+ selectable 
            marker; attB site for genome integration by phiC31, destination 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller 
            NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, 
            Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG
  TITLE     Modular vector assembly enables rapid assessment of emerging CRISPR 
            technologies.
  JOURNAL   Cell Genom. 2024 Mar 13;4(3):100519. doi: 
            10.1016/j.xgen.2024.100519.
  PUBMED    38484704
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-03-13"; volume: "4(3):100519. doi"; pages: " 
            10.1016/j.xgen.2024.100519"
COMMENT     Sequence Label: pRDB_053
FEATURES             Location/Qualifiers
     source          1..9652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    229..659
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     intron          1395..1460
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1590..1610
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1882..2003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1919..1938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1973..1992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2025..2455
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(2474..2494)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2474..2492)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2513..2529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2513..2529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2526..2548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2537..2553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2561..2567,2568..2585,2586..2591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2606..2627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2744..2761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2915..3503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2995..3014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3674..4465,4466..4534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4297..4316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4535..4639)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4666..5122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4753..4772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4963..4984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5248..5270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5262..5279
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5263..5279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5289..5308
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5289..5307
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(6111..6725,6796..6927,7131..7446,7508..8162,
                     8237..8510,8912..8983))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9433..9451
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9574..9643
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
       61 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
      121 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
      181 tcgacaagcc gaattgatcc actagaaggc ctaattcggt acactagttg gccacgtaat
      241 aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt aaaattttgt
      301 tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa taatttttta
      361 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
      421 actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacccg
      481 tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata cgttgacgaa
      541 acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt tcttgccata
      601 ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca accaacaacc
      661 agaacgtctt caccagtaat ttacgtggga aaggtgcttg tgtcccaaca ggctaggata
      721 taatgctgaa accgtccccc aagcgttcag ggtggggttt gctacaactt ccgagtccaa
      781 tgtgtccgtg ttcatgatat atatgctcaa gggcgagaat tggacctagc tttcgtgtta
      841 gtacgtagca tggtgacaca agcacagtag atcctgcccg ccgatcgtat atattaagtt
      901 aattcttatg gaatataata acatgtggat ggccagtggt cggttgttac acgcctaccg
      961 cgatgctgaa tgacccggac tagagtggcg aaatttatgg cgtgtgaccc gttatgctcc
     1021 atttcggtca gtgggtcatt gctagtagtc gattgcattg ccattctccg agtgatttat
     1081 ggaccccagt gaagacgcat tctctagagc aaactagttc tgatctgcta gacaattgtt
     1141 ggcatcaggt aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg
     1201 aaaatatgtt tttgtataca tttttgatat tttcaaataa tacgcagtta taaaactcat
     1261 tagctaaccc attttttctt tgcttatgct tacagattgc aaagaactag agccgcggga
     1321 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     1381 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     1441 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     1501 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     1561 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gtagacccca
     1621 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     1681 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     1741 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1801 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1861 atgaatggaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1981 tccaaactca tcaatgtatc ttatcatgtc tggttccaga gctctggcca cgtaataagt
     2041 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     2101 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     2161 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     2221 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     2281 taataaaata cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa
     2341 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
     2401 ttagccgatc aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacgagct
     2461 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     2521 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2581 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2701 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3541 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3601 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     4681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4801 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5041 tttcggccta ttggttaaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5101 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     5161 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     5221 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     5281 agcgcgcgta atacgactca ctatagggcg aattgggtac aagcttcacg acctaggcgc
     5341 gccctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta ttattatagt
     5401 tgttattttt gatatatata aacaacacta ttatgcccac catttttttg agatgcatct
     5461 acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta atcactccga
     5521 acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa atatagctgt
     5581 gagggaaata tatacaaata tattggagca aataaattgt acatacaaat atttattact
     5641 aatttctatt gagtcgaaat gaaccactcg gaaccatttg agcgaaccga atcgcgcgga
     5701 actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt
     5761 gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg
     5821 cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg tagcgataaa
     5881 cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaaa caaatccaaa
     5941 gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca tatcttgttt
     6001 ttattggcac aaatataatt acattagctt tttgaggggg caataaacag taaacacgat
     6061 ggtaataatg gtaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     6121 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     6181 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ttccaggatg
     6241 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     6301 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     6361 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     6421 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     6481 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     6541 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     6601 aaaaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     6661 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     6721 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
     6781 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
     6841 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
     6901 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
     6961 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
     7021 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
     7081 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
     7141 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
     7201 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
     7261 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
     7321 atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc ccgtccgggc
     7381 acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg acactgggca
     7441 cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat aaacacatcg
     7501 aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag ctaccctgcc
     7561 ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaggacg gctgatgaat
     7621 ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga cgacgctgtg
     7681 ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg gcggatcggt
     7741 tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac ctttcaccct
     7801 gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct ggatcacctg
     7861 atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca ccatggcttg
     7921 gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct gctggacata
     7981 ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca gtcgcatccc
     8041 ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca gcagggtcgt
     8101 ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg ccacgccgca
     8161 aactgagttt caaattggta attggaccct ttattaagat ttcacacaga tcagccgact
     8221 gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg
     8281 ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc cggctgattg
     8341 accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc tagttggccg
     8401 ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta gtttttggcc
     8461 tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat
     8521 aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac aaaaagtacc
     8581 ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat ctaggatcag
     8641 cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa atgaatgcct
     8701 ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag aacagctacc
     8761 agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga tttttgtggg
     8821 tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat agatcaagtg
     8881 aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca gatgggtgtt
     8941 tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg atttttcaca
     9001 ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa aaacaaacaa
     9061 aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg gcgtaaaccg
     9121 cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca ttgacgctac
     9181 gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga gtcggcgata
     9241 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
     9301 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
     9361 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaatgttttt tttatctact
     9421 ttccgcaaaa atgggtttta ttaacttaca tacatactag gcgcgccaga tcgcaagaag
     9481 cttgatatca tcgatctcga ggctgcatcc aacgcgttgg gagctctccg gatcaattcg
     9541 gcttcaggta ccgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg
     9601 cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca tc
//
