LOCUS       sequence-431441-        4728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fragmid fragment: (N' terminus) catalytic core of human 
            acetyltransferase p300.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller 
            NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, 
            Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG
  TITLE     Modular vector assembly enables rapid assessment of emerging CRISPR 
            technologies.
  JOURNAL   Cell Genom. 2024 Mar 13;4(3):100519. doi: 
            10.1016/j.xgen.2024.100519.
  PUBMED    38484704
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-03-13"; volume: "4(3):100519. doi"; pages: " 
            10.1016/j.xgen.2024.100519"
COMMENT     Sequence Label: AA124
FEATURES             Location/Qualifiers
     source          1..4728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2547..2567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2638..2654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2638..2654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2651..2673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2662..2678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2686..2692,2693..2710,2711..2716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2731..2752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2869..2886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3120..3139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3806..4615)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4529..4548
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4607..4626
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggccatttt caagcctgaa gagctgagac
      481 aagccctgat gcccaccctg gaggccctgt atcggcagga ccctgagagc ctgcccttca
      541 gacagcctgt ggatcctcag ctgctgggaa tccctgatta cttcgacatc gttaagtctc
      601 ctatggatct gtctaccatc aaacgcaagc tggacaccgg ccagtaccag gagccttggc
      661 agtacgtgga cgacatctgg ctgatgttca acaacgcctg gctgtacaac agaaagacca
      721 gcagagtgta caagtactgc agcaagctga gcgaggtctt tgagcaagag atcgaccctg
      781 tgatgcagtc tctgggctat tgttgcggca gaaagctgga attcagccct caaacactgt
      841 gctgctacgg caagcagctg tgcaccatcc cacgggatgc tacatactac agctaccaga
      901 atagatacca cttctgcgag aagtgcttta acgagattca gggcgagtcc gtgtccctgg
      961 gcgacgaccc cagccagccc cagaccacca tcaacaaaga gcagttcagc aagcgcaaga
     1021 acgacaccct ggatcccgag ctttttgtgg aatgtaccga gtgcggcagg aagatgcacc
     1081 agatctgcgt gctgcaccac gagatcatct ggcccgccgg cttcgtgtgc gacggctgtc
     1141 tgaagaagag cgccagaacc cggaaagaga acaagttcag tgctaagaga ctgcctagca
     1201 ccagactggg aacattcctg gaaaataggg tgaacgactt cctgcggcgg caaaaccacc
     1261 cggagtctgg agaagtcacc gtgcgggtgg tgcacgccag cgacaagacc gtggaagtga
     1321 aacctggcat gaaggccaga ttcgtggaca gcggagagat ggccgagagc ttcccctaca
     1381 gaacaaaggc cctgttcgcc ttcgaggaaa tcgacggtgt tgatctgtgt tttttcggca
     1441 tgcatgtgca ggagtacggc tctgactgcc ctccaccaaa ccagagaaga gtctatatca
     1501 gctacctgga ctctgtgcat ttcttccggc ccaaatgcct gagaacagcc gtgtaccacg
     1561 agatcctcat cggctacctc gagtacgtga agaaactggg ctacaccaca ggccacatct
     1621 gggcctgtcc tccatctgaa ggcgacgact acatcttcca ctgccaccct cctgaccaga
     1681 aaatccctaa gcccaagaga ctgcaggagt ggtacaaaaa aatgctggac aaggctgtga
     1741 gcgaaagaat cgtgcacgac tacaaggaca tctttaagca ggccactgaa gatagactga
     1801 cctccgccaa ggagctgcct tacttcgagg gcgatttctg gcctaatgtg ctggaggaat
     1861 ctatcaagga actggagcag gaggaggaag agcggaagcg ggaagaaaat acctccaacg
     1921 agtctacaga cgtgaccaag ggcgacagca agaacgctaa gaagaaaaac aacaagaaaa
     1981 ccagcaaaaa caagagcagc ctgtccagag gcaacaaaaa aaagcctgga atgcctaacg
     2041 tcagcaacga cctgagccag aagctgtacg ccacaatgga aaagcacaag gaagtgttct
     2101 ttgtgatcag actgattgcc ggacctgccg ccaatagcct gcctcccatc gtcgatccag
     2161 accccctgat cccctgtgat ctgatggacg gcagagatgc tttcctgacc ctggccagag
     2221 ataagcacct ggaattcagc agcctgagaa gagcccagtg gagcaccatg tgcatgctgg
     2281 tggaactgca cacacagagc caggacggag ggccgagctc tggcgcaccc ccaccaagtg
     2341 gagggtctcc tgccgggtcc ccaacatcta ctgaagaagg caccagcgaa tccgcaacgc
     2401 ccgagtcagg ccctggtacc tccacagaac catctgaagg tagtgcgcct ggttccccag
     2461 ctggaagccc tacttccacc gaagaaggca cgtcaaccga accaagtgaa ggatctgccc
     2521 ctgggaccag cactgaacca tctgagccaa aaaagaagag aaaggtaggc agcagtcttc
     2581 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggtgt aatcatggtc
     2641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaagcccaa tctgaataat
     3781 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     3841 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     3901 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     3961 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     4021 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     4081 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     4141 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     4201 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     4261 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga
     4321 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     4381 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     4441 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat
     4501 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     4561 catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac
     4621 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     4681 cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgca
//
