LOCUS       sequence-43154-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Slavoff SA, Liu DS, Cohen JD, Ting AY
  TITLE     Imaging Protein-Protein Interactions inside Living Cells via 
            Interaction-Dependent Fluorophore Ligation.
  JOURNAL   J Am Chem Soc. 2011 Dec 14;133(49):19769-76. Epub 2011 Nov 18.
  PUBMED    22098454
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2011-12-14"; volume: "133(49)"; pages: "19769-76. Epub
            2011 Nov 18"
COMMENT     Sequence Label: pcDNA3-FRB-LplA(W37I)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             468..491
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgcggccg ccaccatggt gcggggttct catcatcatc
      121 atcatcatgg tatggctagc tatccgtacg acgtaccaga ctacgcaatg tggcatgaag
      181 gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg
      241 tgctggagcc cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct
      301 ttaatcaggc ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga
      361 aatcagggaa tgtcaaggac ctcacccaag cctgggacct ctattatcat gtgttccgac
      421 gaatctcaaa gggctcgggc tcgacctcgg gctcgggcaa ggatccagac tacaaggatg
      481 acgacgataa gatgagcacc ctgagactgc tgatcagcga cagctacgac ccctggttca
      541 acctggccgt ggaggagtgc atcttcagac agatgcccgc cacccagaga gtgctgttcc
      601 tgatcagaaa cgccgacacc gtggtgatcg gcagagccca gaacccctgg aaggagtgca
      661 acaccagaag aatggaggag gacaacgtga gactggccag aagaagcagc ggcggcggcg
      721 ccgtgttcca cgacctgggc aacacctgct tcaccttcat ggccggcaag cccgagtacg
      781 acaagaccat cagcaccagc atcgtgctga acgccctgaa cgccctgggc gtgagcgccg
      841 aggccagcgg cagaaacgac ctggtggtga agaccgtgg
//
