LOCUS       sequence-431553-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike Q183E for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 Q183E
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3840..4428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3893..3913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4311..4322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4481..4602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4492..4511)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4546..4565
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4717..4763
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4793..4929
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4974..5045
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5090..5286
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5137..5272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5300..6094
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5354..5373)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5964..5983
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6105..6329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6525..7113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6605..6624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7859..7927,7928..8719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8077..8096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8732..8868
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9020..9091
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9096..9142)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9160..9208
                     /note="synthetic polyadenylation signal"
     enhancer        9289..9592
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9594..9796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9747..9767
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9930..10405
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 catgtttgtg ttcctcgttc tcctgcccct cgtttcttcc caatgcgtga acctcacaac
       61 tagaacgcag ctcccacccg cttacacgaa ctcttttact cgtggagtat actatcccga
      121 taaagtcttt aggtccagtg tccttcattc tactcaagac cttttcctgc cattcttctc
      181 aaatgtcacc tggttccatg ctatccacgt ttctggcacc aacggaacta aacgatttga
      241 taatcccgtc ttgccattta acgatggcgt atactttgcc tcaacggaga agtccaacat
      301 tatccgggga tggatatttg gcaccaccct ggactccaag acacagtctc tcctcattgt
      361 gaacaacgcc accaatgtcg tcatcaaagt ctgtgaattt caattttgta acgatccctt
      421 tctcggggtt tattatcata agaataacaa gtcttggatg gagtcagagt tccgcgttta
      481 cagttccgct aacaactgta cttttgagta cgtttcccaa ccatttctca tggatcttga
      541 agggaaggaa ggcaacttca agaatctccg cgaatttgtg tttaagaata tagacggcta
      601 cttcaaaatt tatagcaagc ataccccgat aaatctggtt cgggatctcc ctcaaggctt
      661 ctccgcgttg gaacccctcg tggacttgcc gattggcatc aacatcaccc ggttccaaac
      721 ccttcttgcc cttcatcgaa gctacctcac gcctggcgac agtagtagtg gttggacagc
      781 gggcgcagca gcctactatg tgggatattt gcaaccacgc acctttcttc tcaaatataa
      841 cgaaaacgga acaattaccg atgccgtaga ctgtgccctc gatcccctgt ccgaaactaa
      901 atgcacactc aagtccttca cagttgagaa aggcatatac caaaccagta acttccgcgt
      961 ccaacccacc gaatccattg ttcgattccc taacatcacc aatttgtgtc ctttcggtga
     1021 agtatttaat gccactcgat ttgcatcagt ttatgcctgg aatcgcaagc gaatatcaaa
     1081 ttgtgttgca gattatagtg tcctgtataa tagcgcatca ttcagcactt ttaagtgtta
     1141 tggggtcagc cctacgaaat tgaatgactt gtgttttacc aacgtttacg cagattcttt
     1201 cgtcatacgc ggcgacgaag tccgacagat tgcaccgggg caaaccggaa agatagcaga
     1261 ctataattat aagttgccgg atgacttcac cggctgcgtt atagcatgga actccaataa
     1321 tctcgattca aaagtcggcg gcaactataa ttatctttac cgcctgtttc gcaagtcaaa
     1381 tctgaagccc tttgaacgtg acatctccac cgaaatttat caagctggca gcaccccatg
     1441 taatggagtg gaagggttta actgttattt tccccttcaa tcatacggat ttcaaccaac
     1501 aaatggggtg ggctatcaac catatcgggt ggtagtcttg agctttgagc tgctccacgc
     1561 accggctaca gtttgcggac cgaagaaaag cactaacctg gtgaagaata aatgtgtgaa
     1621 cttcaacttt aatgggctca ccggaacagg ggtgctgaca gaatcaaaca agaaatttct
     1681 cccttttcaa caatttggta gagacattgc cgataccaca gatgccgtcc gcgatccgca
     1741 aacactggaa atcctcgata taacaccctg tagcttcggc ggggtcagcg tgataactcc
     1801 cggaacgaac acatccaacc aagtggctgt cctgtatcaa gatgtcaact gtaccgaagt
     1861 ccctgtggcg atacacgccg accaactgac acccacctgg agagtctact caacaggttc
     1921 caatgtcttt caaactagag cgggttgtct cattggcgct gagcatgtca ataattcata
     1981 cgaatgtgat attcctattg gggcaggaat ctgtgcgtct tatcaaactc aaacgaactc
     2041 accacggcgc gctcgctcag tagccagcca gtccataatc gcctatacca tgagtctcgg
     2101 ggctgagaac tccgtggctt attcaaacaa ttccatcgca atcccaacta actttaccat
     2161 tagcgttacc acggaaatct tgcccgtcag catgactaag acgagcgtag actgtacgat
     2221 gtatatctgt ggggattcca ccgagtgtag caacctcttg cttcaatacg ggtctttctg
     2281 tactcaactc aaccgagcat tgaccggaat tgcggtcgaa caagacaaga atacccaaga
     2341 ggtgttcgca caagtaaagc aaatatacaa aacgccgcca atcaaagatt ttggcgggtt
     2401 taattttagc caaatactgc cagacccatc taagccttca aaacgcagct ttatcgagga
     2461 tctgctgttc aacaaagtta cgttggctga cgcgggtttc attaagcagt atggggattg
     2521 tttgggagac attgcggccc gcgaccttat ctgtgctcaa aaattcaatg ggttgacagt
     2581 tctgccccct ctgctcactg atgagatgat tgcacagtat acatcagcat tgttggcagg
     2641 aaccattaca tccggctgga cttttggcgc gggtgcggcc cttcaaatac ctttcgctat
     2701 gcaaatggct tacagattca atgggattgg tgtcacgcaa aatgtattgt atgaaaatca
     2761 gaagctgatc gctaatcaat ttaacagcgc gattggcaag atccaagatt cactgtcaag
     2821 cacagcgtcc gccctcggca aactccaaga tgttgtgaat caaaacgccc aggccctgaa
     2881 taccctcgtg aagcaactga gttctaactt cggcgcaatc tccagcgtcc tcaacgacat
     2941 attgtccaga cttgataagg tggaagccga ggtgcaaatt gatcggctca tcacaggcag
     3001 gctccagtca ctccaaactt atgtgacaca acagttgatc cgggctgctg agatacgagc
     3061 aagcgccaac ctggccgcca ctaaaatgtc agagtgcgtg ctcgggcagt ccaaacgggt
     3121 tgacttttgt ggcaagggct atcacttgat gtccttcccc caatccgccc cacatggagt
     3181 ggtctttctc cacgtcactt atgtccctgc tcaagagaag aactttacga cagctccagc
     3241 aatctgtcac gatggcaaag cgcactttcc cagggaaggc gttttcgtca gtaatggcac
     3301 gcattggttt gtgacccaaa ggaatttcta tgaaccccaa atcataacca ctgacaatac
     3361 atttgtctca ggcaactgtg atgtggtcat tgggatcgtg aacaacacgg tatatgatcc
     3421 cctgcaaccc gagttggata gcttcaaaga ggaattggat aaatacttta agaatcatac
     3481 ttcacccgat gttgatctcg gggacatatc aggaataaat gcaagtgtcg tcaacattca
     3541 gaaagagatt gatcgcctta atgaggtcgc caagaacttg aatgaatctt tgatcgactt
     3601 gcaagaactg ggcaaatacg aacaatatat taaatggccc tggtacattt ggttgggctt
     3661 tatcgccgga cttatagcta tcgttatggt cacaattatg ttgtgctgta tgactagctg
     3721 ttgttcctgc ttgaaaggat gttgcagttg cggatcttgt tgtaaataaa ggatcctcta
     3781 gaagttgtct cctcctgcac tgactgactg atacaatcga tttctggatc cgcaggccta
     3841 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3901 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3961 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4021 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4081 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4141 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4201 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4261 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4321 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4381 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gtctagcttg
     4441 actgactgag atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt
     4501 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     4561 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4621 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     4681 tacaaatgtg gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta
     4741 aacgggtctt gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc
     4801 gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca
     4861 gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt
     4921 tatccttaag gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct
     4981 ttaatggtct tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct
     5041 ccctaatctc attggttacc ttgggctatc gaaacttaat taagcgatct gcatctcaat
     5101 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5161 tccgcccatt ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc
     5221 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5281 tgcaaaggag gtagccaaca tgattgaaca agatggattg cacgcaggtt ctcccgccgc
     5341 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5401 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5461 cggtgccctg aatgaactcc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5521 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5581 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5641 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5701 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5761 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5821 caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc
     5881 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5941 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6001 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6061 cgccttctat cgccttcttg acgagttctt ctagtatgta agccctgtgc cttctagttg
     6121 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6181 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6241 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6301 gcatgctggg gatgcggtgg gctctatggt taattaacca gtcaagtcag ctacttggcg
     6361 agatcgactt gtctgggttt cgactacgct cagaattgcg tcagtcaagt tcgatctggt
     6421 ccttgctatt gcacccgttc tccgattacg agtttcattt aaatcatgtg agcaaaaggc
     6481 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6541 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6601 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6661 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6721 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6781 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6841 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6961 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7321 atctgtctat ttcgttcatc catagttgca tttaaatttc cgaactctcc aaggccctcg
     7381 tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag gctacctctc gaacgaacta
     7441 tcgcaagtct cttggccggc cttgcgcctt ggctattgct tggcagcgcc tatcgccagg
     7501 tattactcca atcccgaata tccgagatcg ggatcacccg agagaagttc aacctacatc
     7561 ctcaatcccg atctatccga gatccgagga atatcgaaat cggggcgcgc ctggtgtacc
     7621 gagaacgatc ctctcagtgc gagtctcgac gatccatatc gttgcttggc agtcagccag
     7681 tcggaatcca gcttgggacc caggaagtcc aatcgtcaga tattgtactc aagcctggtc
     7741 acggcagcgt accgatctgt ttaaacctag atattgatag tctgatcggt caacgtataa
     7801 tcgagtccta gcttttgcaa acatctatca agagacagga tcagcaggag gctttcgcat
     7861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcgcg
     7981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8041 agaacgcttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8161 tgagtattca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgattgg
     8281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8401 tgtagcaatg gcaacaacct tgcgtaaact attaactggc gaactactta ctctagcttc
     8461 ccggcaacag ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8701 actgattaag cattggtaac cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc
     8761 gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca
     8821 acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaaga tcccgaatcg
     8881 tttaaactcg actctggctc tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt
     8941 ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg
     9001 cagcgatcgc ggctcccgac atcttggacc attagctcca caggtatctt cttccctcta
     9061 gtggtcataa cagcagcttc agctacctct caattcaaaa aacccctcaa gacccgttta
     9121 gaggccccaa ggggttatgc tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca
     9181 tccatcttcg atggatagcg attttattat ctaactgctg atcgagtgta gccagatcta
     9241 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9301 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9361 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9421 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     9481 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     9541 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tgctgatgcg
     9601 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9661 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9721 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9781 ctatataagc agagctggtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     9841 ttttgacctc catagaagac accgggaccg atccagcctc ccctcgaagc ttacatgtgg
     9901 taccgagctc ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
     9961 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
    10021 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
    10081 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
    10141 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
    10201 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
    10261 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
    10321 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
    10381 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
    10441 tcactttggc aaaggcggcc gc
//
