LOCUS       sequence-43157-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Slavoff SA, Liu DS, Cohen JD, Ting AY
  TITLE     Imaging Protein-Protein Interactions inside Living Cells via 
            Interaction-Dependent Fluorophore Ligation.
  JOURNAL   J Am Chem Soc. 2011 Dec 14;133(49):19769-76. Epub 2011 Nov 18.
  PUBMED    22098454
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2011-12-14"; volume: "133(49)"; pages: "19769-76. Epub
            2011 Nov 18"
COMMENT     Sequence Label: pcDNA4-FKBP-LAP1
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             162..194
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(198..206,207..221)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             228..257
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             258..578
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(741..758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttgcggccg
      121 ccaccatggt gcggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
      181 gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccggaa caaaaactta
      241 tttctgaaga agatctggga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct
      301 tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga
      361 aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg
      421 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga
      481 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg
      541 ccactctcgt cttcgatgtg gagcttctaa aactggaagg ctctggctcg acctcgggct
      601 cgggcaaaga cgaagtactg gttgaaatcg aaaccgacaa agcagttctg gaagtaccgt
      661 aaaggaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
      721 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      781 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctat
//
