LOCUS       sequence-431578-       10459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike E484 deletion for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 del E484
FEATURES             Location/Qualifiers
     source          1..10459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3837..4425
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3890..3910)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4308..4319)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4478..4599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4489..4508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4543..4562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4714..4760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4790..4926
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4971..5042
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5087..5283
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5134..5269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5297..6091
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5351..5370)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5961..5980
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6102..6326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6522..7110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6602..6621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7856..7924,7925..8716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8074..8093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8729..8865
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9017..9088
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9093..9139)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9157..9205
                     /note="synthetic polyadenylation signal"
     enhancer        9286..9589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9591..9793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9744..9764
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9927..10402
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 catgtttgtg ttcctcgttc tcctgcccct cgtttcttcc caatgcgtga acctcacaac
       61 tagaacgcag ctcccacccg cttacacgaa ctcttttact cgtggagtat actatcccga
      121 taaagtcttt aggtccagtg tccttcattc tactcaagac cttttcctgc cattcttctc
      181 aaatgtcacc tggttccatg ctatccacgt ttctggcacc aacggaacta aacgatttga
      241 taatcccgtc ttgccattta acgatggcgt atactttgcc tcaacggaga agtccaacat
      301 tatccgggga tggatatttg gcaccaccct ggactccaag acacagtctc tcctcattgt
      361 gaacaacgcc accaatgtcg tcatcaaagt ctgtgaattt caattttgta acgatccctt
      421 tctcggggtt tattatcata agaataacaa gtcttggatg gagtcagagt tccgcgttta
      481 cagttccgct aacaactgta cttttgagta cgtttcccaa ccatttctca tggatcttga
      541 agggaagcaa ggcaacttca agaatctccg cgaatttgtg tttaagaata tagacggcta
      601 cttcaaaatt tatagcaagc ataccccgat aaatctggtt cgggatctcc ctcaaggctt
      661 ctccgcgttg gaacccctcg tggacttgcc gattggcatc aacatcaccc ggttccaaac
      721 ccttcttgcc cttcatcgaa gctacctcac gcctggcgac agtagtagtg gttggacagc
      781 gggcgcagca gcctactatg tgggatattt gcaaccacgc acctttcttc tcaaatataa
      841 cgaaaacgga acaattaccg atgccgtaga ctgtgccctc gatcccctgt ccgaaactaa
      901 atgcacactc aagtccttca cagttgagaa aggcatatac caaaccagta acttccgcgt
      961 ccaacccacc gaatccattg ttcgattccc taacatcacc aatttgtgtc ctttcggtga
     1021 agtatttaat gccactcgat ttgcatcagt ttatgcctgg aatcgcaagc gaatatcaaa
     1081 ttgtgttgca gattatagtg tcctgtataa tagcgcatca ttcagcactt ttaagtgtta
     1141 tggggtcagc cctacgaaat tgaatgactt gtgttttacc aacgtttacg cagattcttt
     1201 cgtcatacgc ggcgacgaag tccgacagat tgcaccgggg caaaccggaa agatagcaga
     1261 ctataattat aagttgccgg atgacttcac cggctgcgtt atagcatgga actccaataa
     1321 tctcgattca aaagtcggcg gcaactataa ttatctttac cgcctgtttc gcaagtcaaa
     1381 tctgaagccc tttgaacgtg acatctccac cgaaatttat caagctggca gcaccccatg
     1441 taatggagtg gggtttaact gttattttcc ccttcaatca tacggatttc aaccaacaaa
     1501 tggggtgggc tatcaaccat atcgggtggt agtcttgagc tttgagctgc tccacgcacc
     1561 ggctacagtt tgcggaccga agaaaagcac taacctggtg aagaataaat gtgtgaactt
     1621 caactttaat gggctcaccg gaacaggggt gctgacagaa tcaaacaaga aatttctccc
     1681 ttttcaacaa tttggtagag acattgccga taccacagat gccgtccgcg atccgcaaac
     1741 actggaaatc ctcgatataa caccctgtag cttcggcggg gtcagcgtga taactcccgg
     1801 aacgaacaca tccaaccaag tggctgtcct gtatcaagat gtcaactgta ccgaagtccc
     1861 tgtggcgata cacgccgacc aactgacacc cacctggaga gtctactcaa caggttccaa
     1921 tgtctttcaa actagagcgg gttgtctcat tggcgctgag catgtcaata attcatacga
     1981 atgtgatatt cctattgggg caggaatctg tgcgtcttat caaactcaaa cgaactcacc
     2041 acggcgcgct cgctcagtag ccagccagtc cataatcgcc tataccatga gtctcggggc
     2101 tgagaactcc gtggcttatt caaacaattc catcgcaatc ccaactaact ttaccattag
     2161 cgttaccacg gaaatcttgc ccgtcagcat gactaagacg agcgtagact gtacgatgta
     2221 tatctgtggg gattccaccg agtgtagcaa cctcttgctt caatacgggt ctttctgtac
     2281 tcaactcaac cgagcattga ccggaattgc ggtcgaacaa gacaagaata cccaagaggt
     2341 gttcgcacaa gtaaagcaaa tatacaaaac gccgccaatc aaagattttg gcgggtttaa
     2401 ttttagccaa atactgccag acccatctaa gccttcaaaa cgcagcttta tcgaggatct
     2461 gctgttcaac aaagttacgt tggctgacgc gggtttcatt aagcagtatg gggattgttt
     2521 gggagacatt gcggcccgcg accttatctg tgctcaaaaa ttcaatgggt tgacagttct
     2581 gccccctctg ctcactgatg agatgattgc acagtataca tcagcattgt tggcaggaac
     2641 cattacatcc ggctggactt ttggcgcggg tgcggccctt caaatacctt tcgctatgca
     2701 aatggcttac agattcaatg ggattggtgt cacgcaaaat gtattgtatg aaaatcagaa
     2761 gctgatcgct aatcaattta acagcgcgat tggcaagatc caagattcac tgtcaagcac
     2821 agcgtccgcc ctcggcaaac tccaagatgt tgtgaatcaa aacgcccagg ccctgaatac
     2881 cctcgtgaag caactgagtt ctaacttcgg cgcaatctcc agcgtcctca acgacatatt
     2941 gtccagactt gataaggtgg aagccgaggt gcaaattgat cggctcatca caggcaggct
     3001 ccagtcactc caaacttatg tgacacaaca gttgatccgg gctgctgaga tacgagcaag
     3061 cgccaacctg gccgccacta aaatgtcaga gtgcgtgctc gggcagtcca aacgggttga
     3121 cttttgtggc aagggctatc acttgatgtc cttcccccaa tccgccccac atggagtggt
     3181 ctttctccac gtcacttatg tccctgctca agagaagaac tttacgacag ctccagcaat
     3241 ctgtcacgat ggcaaagcgc actttcccag ggaaggcgtt ttcgtcagta atggcacgca
     3301 ttggtttgtg acccaaagga atttctatga accccaaatc ataaccactg acaatacatt
     3361 tgtctcaggc aactgtgatg tggtcattgg gatcgtgaac aacacggtat atgatcccct
     3421 gcaacccgag ttggatagct tcaaagagga attggataaa tactttaaga atcatacttc
     3481 acccgatgtt gatctcgggg acatatcagg aataaatgca agtgtcgtca acattcagaa
     3541 agagattgat cgccttaatg aggtcgccaa gaacttgaat gaatctttga tcgacttgca
     3601 agaactgggc aaatacgaac aatatattaa atggccctgg tacatttggt tgggctttat
     3661 cgccggactt atagctatcg ttatggtcac aattatgttg tgctgtatga ctagctgttg
     3721 ttcctgcttg aaaggatgtt gcagttgcgg atcttgttgt aaataaagga tcctctagaa
     3781 gttgtctcct cctgcactga ctgactgata caatcgattt ctggatccgc aggcctaatc
     3841 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3901 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3961 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4021 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4081 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4141 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4201 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4261 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4321 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4381 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc tagcttgact
     4441 gactgagata cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga
     4501 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4561 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4621 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     4681 aaatgtggta ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac
     4741 gggtcttgag gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca
     4801 gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca
     4861 acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
     4921 ccttaaggtc gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta
     4981 atggtctttt ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc
     5041 taatctcatt ggttaccttg ggctatcgaa acttaattaa gcgatctgca tctcaattag
     5101 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5161 gcccattctc cgccccatcg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5281 aaaggaggta gccaacatga ttgaacaaga tggattgcac gcaggttctc ccgccgcttg
     5341 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5401 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5461 tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5521 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5581 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5641 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5701 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5761 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5821 ggcgcggatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa
     5881 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5941 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6001 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6061 cttctatcgc cttcttgacg agttcttcta gtatgtaagc cctgtgcctt ctagttgcca
     6121 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6181 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6241 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6301 tgctggggat gcggtgggct ctatggttaa ttaaccagtc aagtcagcta cttggcgaga
     6361 tcgacttgtc tgggtttcga ctacgctcag aattgcgtca gtcaagttcg atctggtcct
     6421 tgctattgca cccgttctcc gattacgagt ttcatttaaa tcatgtgagc aaaaggccag
     6481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6961 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7321 tgtctatttc gttcatccat agttgcattt aaatttccga actctccaag gccctcgtcg
     7381 gaaaatcttc aaacctttcg tccgatccat cttgcaggct acctctcgaa cgaactatcg
     7441 caagtctctt ggccggcctt gcgccttggc tattgcttgg cagcgcctat cgccaggtat
     7501 tactccaatc ccgaatatcc gagatcggga tcacccgaga gaagttcaac ctacatcctc
     7561 aatcccgatc tatccgagat ccgaggaata tcgaaatcgg ggcgcgcctg gtgtaccgag
     7621 aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt gcttggcagt cagccagtcg
     7681 gaatccagct tgggacccag gaagtccaat cgtcagatat tgtactcaag cctggtcacg
     7741 gcagcgtacc gatctgttta aacctagata ttgatagtct gatcggtcaa cgtataatcg
     7801 agtcctagct tttgcaaaca tctatcaaga gacaggatca gcaggaggct ttcgcatgag
     7861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcgcgagt
     7981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8041 acgctttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8161 gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg
     8281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8401 agcaatggca acaaccttgc gtaaactatt aactggcgaa ctacttactc tagcttcccg
     8461 gcaacagttg atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8701 gattaagcat tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac
     8761 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact
     8821 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagatcc cgaatcgttt
     8881 aaactcgact ctggctctat cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc
     8941 gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc tttttggcag
     9001 cgatcgcggc tcccgacatc ttggaccatt agctccacag gtatcttctt ccctctagtg
     9061 gtcataacag cagcttcagc tacctctcaa ttcaaaaaac ccctcaagac ccgtttagag
     9121 gccccaaggg gttatgctat caatcgttgc gttacacaca caaaaaacca acacacatcc
     9181 atcttcgatg gatagcgatt ttattatcta actgctgatc gagtgtagcc agatctagta
     9241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     9301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     9361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     9421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     9481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     9541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc tgatgcggtt
     9601 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     9661 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     9721 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     9781 tataagcaga gctggtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     9841 tgacctccat agaagacacc gggaccgatc cagcctcccc tcgaagctta catgtggtac
     9901 cgagctcgga tcctgagaac ttcagggtga gtctatggga cccttgatgt tttctttccc
     9961 cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg gtacacatat
    10021 tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat
    10081 acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat
    10141 gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt
    10201 taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta
    10261 agaggtttca tattgctaat agcagctaca atccagctac cattctgctt ttattttatg
    10321 gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat
    10381 acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc tggcccatca
    10441 ctttggcaaa ggcggccgc
//
