LOCUS       sequence-431582-       10450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike for HV.1 pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 HV.1
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3827..4415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3880..3900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4298..4309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4468..4589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4479..4498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4533..4552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4704..4750
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4780..4916
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4961..5032
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5077..5273
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5124..5259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5287..6081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5341..5360)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5951..5970
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6092..6316
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6512..7100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6592..6611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7846..7914,7915..8706)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8064..8083)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8719..8855
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9007..9078
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9083..9129)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9147..9195
                     /note="synthetic polyadenylation signal"
     enhancer        9276..9579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9581..9783
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9734..9754
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9917..10392
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcataact
       61 agaacgcagt cttacacgaa ctcttttact cgtggagtat actatcccga taaagtcttt
      121 aggtccagtg tccttcattc tactcatgac cttttcctgc cattcttctc aaatgtcacc
      181 tggttccatg ctatccacgt ttctggcacc aacggaacta aacgatttga taatcccgcc
      241 ttgccattta acgatggcgt atactttgcc tcaacggaga agtccaacat tatccgggga
      301 tggatatttg gcaccaccct ggactccaag acacagtctc tcctcattgt gaacaacgcc
      361 accaatgtcg tcatcaaagt ctgtgaattt caattttgta acgatccctt tctcgatgtt
      421 tatcagaaga ataacaagtc ttggatggag tcagagttac gcgtttacag ttccgctaac
      481 aactgtactt ttgagtacgt ttcccaacca tttctcatgg atcttgaagg gaaggaaggc
      541 aacttcaaga atctccgcga atttgtgttt aagaatatag acggctactt caaaatttat
      601 agcaagcata ccccgataaa tctggagcgg gatctccctc aaggcttctc cgcgttggaa
      661 cccctcgtgg acttgccgat tggcatcaac atcacccggt tccaaaccct tcttgccctt
      721 catcgaagct acctcacgcc tgtcgacagt agtagtggtt ggacagcggg cgcagcagcc
      781 tactatgtgg gatatttgca accacgcacc tttcttctca aatataacga aaacggaaca
      841 attaccgatg ccgtagactg tgccctcgat cccctgtccg aaactaaatg cacactcaag
      901 tccttcacag ttgagaaagg catataccaa accagtaact tccgcgtcca acccaccgaa
      961 tccattgttc gattccctaa catcaccaat ttgtgtcctt tccatgaagt atttaatgcc
     1021 actacatttg catcagttta tgcctggaat cgcaagcgaa tatcaaattg tgttgcagat
     1081 tatagtgtca tctataattt cgcaccattc ttcgctttta agtgttatgg ggtcagccct
     1141 acgaaattga atgacttgtg ttttaccaac gtttacgcag attctttcgt catacgcggc
     1201 aacgaagtca gccagattgc accggggcaa accggaaata tagcagacta taattataag
     1261 ttgccggatg acttcaccgg ctgcgttata gcatggaact ccaataagct cgattcaaaa
     1321 cccagcggca actataatta tcggtaccgc ctgttacgca agtcaaagct gaagcccttt
     1381 gaacgtgaca tctccaccga aatttatcaa gctggcaaca aaccatgtaa tggagtggca
     1441 gggcctaact gttattctcc ccttcaatca tacggatttc gaccaacata tggggtgggc
     1501 catcaaccat atcgggtggt agtcttgagc tttgagctgc tccacgcacc ggctacagtt
     1561 tgcggaccga agaaaagcac taacctggtg aagaataaat gtgtgaactt caactttaat
     1621 gggctcaccg gaacaggggt gctgacagaa tcaaacaaga aatttctccc ttttcaacaa
     1681 tttggtagag acattgccga taccacagat gccgtccgcg atccgcaaac actggaaatc
     1741 ctcgatataa caccctgtag cttcggcggg gtcagcgtga taactcccgg aacgaacaca
     1801 tccaaccaag tggctgtcct gtatcaaggt gtcaactgta ccgaagtccc tgtggcgata
     1861 cacgccgacc aactgacacc cacctggaga gtctactcaa caggttccaa tgtctttcaa
     1921 actagagcgg gttgtctcat tggcgctgag tatgtcaata attcatacga atgtgatatt
     1981 cctattgggg caggaatctg tgcgtcttat caaactcaaa cgaaatcaca ccggcgcgct
     2041 cgctcagtag ccagccagtc cataatcgcc tataccatga gtctcggggc tgagaactcc
     2101 gtggcttatt caaacaattc catcgcaatc ccaactaact ttaccattag cgttaccacg
     2161 gaaatcttgc ccgtcagcat gactaagacg agcgtagact gtacgatgta tatctgtggg
     2221 gattccaccg agtgtagcaa cctcttgctt caatacgggt ctttctgtac tcaactcaaa
     2281 cgagcattga ccggaattgc ggtcgaacaa gacaagaata cccaagaggt gttcgcacaa
     2341 gtaaagcaaa tatacaaaac gccgccaatc aaatattttg gcgggtttaa ttttagccaa
     2401 atactgccag acccatctaa gccttcaaaa cgcagcttta tcgaggatct gctgttcaac
     2461 aaagttacgt tggctgacgc gggtttcatt aagcagtatg gggattgttt gggagacatt
     2521 gcggcccgcg accttatctg tgctcaaaaa ttcaatgggt tgacagttct gccccctctg
     2581 ctcactgatg agatgattgc acagtataca tcagcattgt tggcaggaac cattacatcc
     2641 ggctggactt ttggcgcggg tgcggccctt caaatacctt tcgctatgca aatggcttac
     2701 agattcaatg ggattggtgt cacgcaaaat gtattgtatg aaaatcagaa gctgatcgct
     2761 aatcaattta acagcgcgat tggcaagatc caagattcac tgtcaagcac agcgtccgcc
     2821 ctcggcaaac tccaagatgt tgtgaatcat aacgcccagg ccctgaatac cctcgtgaag
     2881 caactgagtt ctaaattcgg cgcaatctcc agcgtcctca acgacatatt gtccagactt
     2941 gataaggtgg aagccgaggt gcaaattgat cggctcatca caggcaggct ccagtcactc
     3001 caaacttatg tgacacaaca gttgatccgg gctgctgaga tacgagcaag cgccaacctg
     3061 gccgccacta aaatgtcaga gtgcgtgctc gggcagtcca aacgggttga cttttgtggc
     3121 aagggctatc acttgatgtc cttcccccaa tccgccccac atggagtggt ctttctccac
     3181 gtcacttatg tccctgctca agagaagaac tttacgacag ctccagcaat ctgtcacgat
     3241 ggcaaagcgc actttcccag ggaaggcgtt ttcgtcagta atggcacgca ttggtttgtg
     3301 acccaaagga atttctatga accccaaatc ataaccactg acaatacatt tgtctcaggc
     3361 aactgtgatg tggtcattgg gatcgtgaac aacacggtat atgatcccct gcaacccgag
     3421 ttggatagct tcaaagagga attggataaa tactttaaga atcatacttc acccgatgtt
     3481 gatctcgggg acatatcagg aataaatgca agtgtcgtca acattcagaa agagattgat
     3541 cgccttaatg aggtcgccaa gaacttgaat gaatctttga tcgacttgca agaactgggc
     3601 aaatacgaac aatatattaa atggccctgg tacatttggt tgggctttat cgccggactt
     3661 atagctatcg ttatggtcac aattatgttg tgctgtatga ctagctgttg ttcctgcttg
     3721 aaaggatgtt gcagttgcgg atcttgttgt aaataaagga tcctctagaa gttgtctcct
     3781 cctgcactga ctgactgata caatcgattt ctggatccgc aggcctaatc aacctctgga
     3841 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3901 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3961 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4021 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4081 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4141 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4201 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4261 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4321 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4381 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc tagcttgact gactgagata
     4441 cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa
     4501 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4561 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4621 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4681 ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac gggtcttgag
     4741 gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca gaaaaaaatg
     4801 cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg
     4861 cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaaggtc
     4921 gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta atggtctttt
     4981 ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc taatctcatt
     5041 ggttaccttg ggctatcgaa acttaattaa gcgatctgca tctcaattag tcagcaacca
     5101 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5161 cgccccatcg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5221 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaggaggta
     5281 gccaacatga ttgaacaaga tggattgcac gcaggttctc ccgccgcttg ggtggagagg
     5341 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5401 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5461 gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5521 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5581 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5641 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5701 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5761 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg
     5821 cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa tatcatggtg
     5881 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5941 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6001 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6061 cttcttgacg agttcttcta gtatgtaagc cctgtgcctt ctagttgcca gccatctgtt
     6121 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6181 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6241 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6301 gcggtgggct ctatggttaa ttaaccagtc aagtcagcta cttggcgaga tcgacttgtc
     6361 tgggtttcga ctacgctcag aattgcgtca gtcaagttcg atctggtcct tgctattgca
     6421 cccgttctcc gattacgagt ttcatttaaa tcatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7321 gttcatccat agttgcattt aaatttccga actctccaag gccctcgtcg gaaaatcttc
     7381 aaacctttcg tccgatccat cttgcaggct acctctcgaa cgaactatcg caagtctctt
     7441 ggccggcctt gcgccttggc tattgcttgg cagcgcctat cgccaggtat tactccaatc
     7501 ccgaatatcc gagatcggga tcacccgaga gaagttcaac ctacatcctc aatcccgatc
     7561 tatccgagat ccgaggaata tcgaaatcgg ggcgcgcctg gtgtaccgag aacgatcctc
     7621 tcagtgcgag tctcgacgat ccatatcgtt gcttggcagt cagccagtcg gaatccagct
     7681 tgggacccag gaagtccaat cgtcagatat tgtactcaag cctggtcacg gcagcgtacc
     7741 gatctgttta aacctagata ttgatagtct gatcggtcaa cgtataatcg agtcctagct
     7801 tttgcaaaca tctatcaaga gacaggatca gcaggaggct ttcgcatgag tattcaacat
     7861 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7921 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcgcgagt gggttacatc
     7981 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgctttcca
     8041 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8101 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtattcacca
     8161 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8221 accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg accgaaggag
     8281 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8341 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8401 acaaccttgc gtaaactatt aactggcgaa ctacttactc tagcttcccg gcaacagttg
     8461 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8521 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8581 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8641 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8701 tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc
     8761 ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc
     8821 catacgtgtc ctccttacca gaaatttatc cttaagatcc cgaatcgttt aaactcgact
     8881 ctggctctat cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc
     8941 tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc tttttggcag cgatcgcggc
     9001 tcccgacatc ttggaccatt agctccacag gtatcttctt ccctctagtg gtcataacag
     9061 cagcttcagc tacctctcaa ttcaaaaaac ccctcaagac ccgtttagag gccccaaggg
     9121 gttatgctat caatcgttgc gttacacaca caaaaaacca acacacatcc atcttcgatg
     9181 gatagcgatt ttattatcta actgctgatc gagtgtagcc agatctagta atcaattacg
     9241 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9301 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9361 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9421 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9481 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9541 tggcagtaca tctacgtatt agtcatcgct attaccatgc tgatgcggtt ttggcagtac
     9601 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9661 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9721 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     9781 gctggtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     9841 agaagacacc gggaccgatc cagcctcccc tcgaagctta catgtggtac cgagctcgga
     9901 tcctgagaac ttcagggtga gtctatggga cccttgatgt tttctttccc cttcttttct
     9961 atggttaagt tcatgtcata ggaaggggag aagtaacagg gtacacatat tgaccaaatc
    10021 agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat acttttttgt
    10081 ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat gatacaatgt
    10141 atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt taaggcaata
    10201 gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta agaggtttca
    10261 tattgctaat agcagctaca atccagctac cattctgctt ttattttatg gttgggataa
    10321 ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat acctcttatc
    10381 ttcctcccac agctcctggg caacgtgctg gtctgtgtgc tggcccatca ctttggcaaa
    10441 ggcggccgcc
//
