LOCUS       sequence-431589-       10459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike Y145H for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 deltaY145
FEATURES             Location/Qualifiers
     source          1..10459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3836..4424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3889..3909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4307..4318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4477..4598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4488..4507)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4542..4561
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4713..4759
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4789..4925
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4970..5041
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5086..5282
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5133..5268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5296..6090
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5350..5369)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5960..5979
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6101..6325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6521..7109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6601..6620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7855..7923,7924..8715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8073..8092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8728..8864
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9016..9087
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9092..9138)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9156..9204
                     /note="synthetic polyadenylation signal"
     enhancer        9285..9588
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9590..9792
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9743..9763
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9926..10401
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgat
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt atcataagaa taacaagtct tggatggagt cagagttccg cgtttacagt
      481 tccgctaaca actgtacttt tgagtacgtt tcccaaccat ttctcatgga tcttgaaggg
      541 aagcaaggca acttcaagaa tctccgcgaa tttgtgttta agaatataga cggctacttc
      601 aaaatttata gcaagcatac cccgataaat ctggttcggg atctccctca aggcttctcc
      661 gcgttggaac ccctcgtgga cttgccgatt ggcatcaaca tcacccggtt ccaaaccctt
      721 cttgcccttc atcgaagcta cctcacgcct ggcgacagta gtagtggttg gacagcgggc
      781 gcagcagcct actatgtggg atatttgcaa ccacgcacct ttcttctcaa atataacgaa
      841 aacggaacaa ttaccgatgc cgtagactgt gccctcgatc ccctgtccga aactaaatgc
      901 acactcaagt ccttcacagt tgagaaaggc atataccaaa ccagtaactt ccgcgtccaa
      961 cccaccgaat ccattgttcg attccctaac atcaccaatt tgtgtccttt cggtgaagta
     1021 tttaatgcca ctcgatttgc atcagtttat gcctggaatc gcaagcgaat atcaaattgt
     1081 gttgcagatt atagtgtcct gtataatagc gcatcattca gcacttttaa gtgttatggg
     1141 gtcagcccta cgaaattgaa tgacttgtgt tttaccaacg tttacgcaga ttctttcgtc
     1201 atacgcggcg acgaagtccg acagattgca ccggggcaaa ccggaaagat agcagactat
     1261 aattataagt tgccggatga cttcaccggc tgcgttatag catggaactc caataatctc
     1321 gattcaaaag tcggcggcaa ctataattat ctttaccgcc tgtttcgcaa gtcaaatctg
     1381 aagccctttg aacgtgacat ctccaccgaa atttatcaag ctggcagcac cccatgtaat
     1441 ggagtggaag ggtttaactg ttattttccc cttcaatcat acggatttca accaacaaat
     1501 ggggtgggct atcaaccata tcgggtggta gtcttgagct ttgagctgct ccacgcaccg
     1561 gctacagttt gcggaccgaa gaaaagcact aacctggtga agaataaatg tgtgaacttc
     1621 aactttaatg ggctcaccgg aacaggggtg ctgacagaat caaacaagaa atttctccct
     1681 tttcaacaat ttggtagaga cattgccgat accacagatg ccgtccgcga tccgcaaaca
     1741 ctggaaatcc tcgatataac accctgtagc ttcggcgggg tcagcgtgat aactcccgga
     1801 acgaacacat ccaaccaagt ggctgtcctg tatcaagatg tcaactgtac cgaagtccct
     1861 gtggcgatac acgccgacca actgacaccc acctggagag tctactcaac aggttccaat
     1921 gtctttcaaa ctagagcggg ttgtctcatt ggcgctgagc atgtcaataa ttcatacgaa
     1981 tgtgatattc ctattggggc aggaatctgt gcgtcttatc aaactcaaac gaactcacca
     2041 cggcgcgctc gctcagtagc cagccagtcc ataatcgcct ataccatgag tctcggggct
     2101 gagaactccg tggcttattc aaacaattcc atcgcaatcc caactaactt taccattagc
     2161 gttaccacgg aaatcttgcc cgtcagcatg actaagacga gcgtagactg tacgatgtat
     2221 atctgtgggg attccaccga gtgtagcaac ctcttgcttc aatacgggtc tttctgtact
     2281 caactcaacc gagcattgac cggaattgcg gtcgaacaag acaagaatac ccaagaggtg
     2341 ttcgcacaag taaagcaaat atacaaaacg ccgccaatca aagattttgg cgggtttaat
     2401 tttagccaaa tactgccaga cccatctaag ccttcaaaac gcagctttat cgaggatctg
     2461 ctgttcaaca aagttacgtt ggctgacgcg ggtttcatta agcagtatgg ggattgtttg
     2521 ggagacattg cggcccgcga ccttatctgt gctcaaaaat tcaatgggtt gacagttctg
     2581 ccccctctgc tcactgatga gatgattgca cagtatacat cagcattgtt ggcaggaacc
     2641 attacatccg gctggacttt tggcgcgggt gcggcccttc aaataccttt cgctatgcaa
     2701 atggcttaca gattcaatgg gattggtgtc acgcaaaatg tattgtatga aaatcagaag
     2761 ctgatcgcta atcaatttaa cagcgcgatt ggcaagatcc aagattcact gtcaagcaca
     2821 gcgtccgccc tcggcaaact ccaagatgtt gtgaatcaaa acgcccaggc cctgaatacc
     2881 ctcgtgaagc aactgagttc taacttcggc gcaatctcca gcgtcctcaa cgacatattg
     2941 tccagacttg ataaggtgga agccgaggtg caaattgatc ggctcatcac aggcaggctc
     3001 cagtcactcc aaacttatgt gacacaacag ttgatccggg ctgctgagat acgagcaagc
     3061 gccaacctgg ccgccactaa aatgtcagag tgcgtgctcg ggcagtccaa acgggttgac
     3121 ttttgtggca agggctatca cttgatgtcc ttcccccaat ccgccccaca tggagtggtc
     3181 tttctccacg tcacttatgt ccctgctcaa gagaagaact ttacgacagc tccagcaatc
     3241 tgtcacgatg gcaaagcgca ctttcccagg gaaggcgttt tcgtcagtaa tggcacgcat
     3301 tggtttgtga cccaaaggaa tttctatgaa ccccaaatca taaccactga caatacattt
     3361 gtctcaggca actgtgatgt ggtcattggg atcgtgaaca acacggtata tgatcccctg
     3421 caacccgagt tggatagctt caaagaggaa ttggataaat actttaagaa tcatacttca
     3481 cccgatgttg atctcgggga catatcagga ataaatgcaa gtgtcgtcaa cattcagaaa
     3541 gagattgatc gccttaatga ggtcgccaag aacttgaatg aatctttgat cgacttgcaa
     3601 gaactgggca aatacgaaca atatattaaa tggccctggt acatttggtt gggctttatc
     3661 gccggactta tagctatcgt tatggtcaca attatgttgt gctgtatgac tagctgttgt
     3721 tcctgcttga aaggatgttg cagttgcgga tcttgttgta aataaaggat cctctagaag
     3781 ttgtctcctc ctgcactgac tgactgatac aatcgatttc tggatccgca ggcctaatca
     3841 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3901 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3961 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4021 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4081 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4141 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4201 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     4261 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4321 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4381 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtct agcttgactg
     4441 actgagatac agcgtacctt cagctcacag acatgataag atacattgat gagtttggac
     4501 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4561 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4621 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     4681 aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg cctctaaacg
     4741 ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc attggcgcag
     4801 aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa
     4861 cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc
     4921 cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac attcctttaa
     4981 tggtcttttc tggacaccac taggggtcag aagtagttca tcaaactttc ttccctccct
     5041 aatctcattg gttaccttgg gctatcgaaa cttaattaag cgatctgcat ctcaattagt
     5101 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5161 cccattctcc gccccatcgc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5221 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5281 aaggaggtag ccaacatgat tgaacaagat ggattgcacg caggttctcc cgccgcttgg
     5341 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5401 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5461 gccctgaatg aactccagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5521 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5581 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5641 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5701 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5761 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5821 gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg gcgatgcctg cttgccgaat
     5881 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5941 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6001 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6061 ttctatcgcc ttcttgacga gttcttctag tatgtaagcc ctgtgccttc tagttgccag
     6121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6301 gctggggatg cggtgggctc tatggttaat taaccagtca agtcagctac ttggcgagat
     6361 cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt
     6421 gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc
     6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7321 gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg
     7381 aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc
     7441 aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt
     7501 actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca
     7561 atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcctgg tgtaccgaga
     7621 acgatcctct cagtgcgagt ctcgacgatc catatcgttg cttggcagtc agccagtcgg
     7681 aatccagctt gggacccagg aagtccaatc gtcagatatt gtactcaagc ctggtcacgg
     7741 cagcgtaccg atctgtttaa acctagatat tgatagtctg atcggtcaac gtataatcga
     7801 gtcctagctt ttgcaaacat ctatcaagag acaggatcag caggaggctt tcgcatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcgcgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caaccttgcg taaactatta actggcgaac tacttactct agcttcccgg
     8461 caacagttga tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaaccgat tctaggtgca ttggcgcaga aaaaaatgcc tgatgcgacg
     8761 ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct tccccaactt
     8821 gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaagatccc gaatcgttta
     8881 aactcgactc tggctctatc gaatctccgt cgtttcgagc ttacgcgaac agccgtggcg
     8941 ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt cagcttacct ttttggcagc
     9001 gatcgcggct cccgacatct tggaccatta gctccacagg tatcttcttc cctctagtgg
     9061 tcataacagc agcttcagct acctctcaat tcaaaaaacc cctcaagacc cgtttagagg
     9121 ccccaagggg ttatgctatc aatcgttgcg ttacacacac aaaaaaccaa cacacatcca
     9181 tcttcgatgg atagcgattt tattatctaa ctgctgatcg agtgtagcca gatctagtaa
     9241 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9301 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9361 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9421 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9481 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9541 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatgct gatgcggttt
     9601 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     9661 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     9721 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     9781 ataagcagag ctggtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     9841 gacctccata gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc
     9901 gagctcggat cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc
     9961 ttcttttcta tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt
    10021 gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata
    10081 cttttttgtt tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg
    10141 atacaatgta tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt
    10201 aaggcaatag caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa
    10261 gaggtttcat attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg
    10321 ttgggataag gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata
    10381 cctcttatct tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac
    10441 tttggcaaag gcggccgcc
//
