LOCUS       sequence-431614-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike deltaH69-H70, Y453F, D614G, I692V, M1229I 
            for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 Danish Mink
            d...
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3833..4421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3886..3906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4304..4315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4474..4595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4485..4504)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4539..4558
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4710..4756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4786..4922
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4967..5038
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5083..5279
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5130..5265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5293..6087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5347..5366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5957..5976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6098..6322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6518..7106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6598..6617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7852..7920,7921..8712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8070..8089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8725..8861
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9013..9084
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9089..9135)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9153..9201
                     /note="synthetic polyadenylation signal"
     enhancer        9282..9585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9587..9789
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9740..9760
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9923..10398
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatctctggc accaacggaa ctaaacgatt tgataatccc
      241 gtcttgccat ttaacgatgg cgtatacttt gcctcaacgg agaagtccaa cattatccgg
      301 ggatggatat ttggcaccac cctggactcc aagacacagt ctctcctcat tgtgaacaac
      361 gccaccaatg tcgtcatcaa agtctgtgaa tttcaatttt gtaacgatcc ctttctcggg
      421 gtttattatc ataagaataa caagtcttgg atggagtcag agttccgcgt ttacagttcc
      481 gctaacaact gtacttttga gtacgtttcc caaccatttc tcatggatct tgaagggaag
      541 caaggcaact tcaagaatct ccgcgaattt gtgtttaaga atatagacgg ctacttcaaa
      601 atttatagca agcatacccc gataaatctg gttcgggatc tccctcaagg cttctccgcg
      661 ttggaacccc tcgtggactt gccgattggc atcaacatca cccggttcca aacccttctt
      721 gcccttcatc gaagctacct cacgcctggc gacagtagta gtggttggac agcgggcgca
      781 gcagcctact atgtgggata tttgcaacca cgcacctttc ttctcaaata taacgaaaac
      841 ggaacaatta ccgatgccgt agactgtgcc ctcgatcccc tgtccgaaac taaatgcaca
      901 ctcaagtcct tcacagttga gaaaggcata taccaaacca gtaacttccg cgtccaaccc
      961 accgaatcca ttgttcgatt ccctaacatc accaatttgt gtcctttcgg tgaagtattt
     1021 aatgccactc gatttgcatc agtttatgcc tggaatcgca agcgaatatc aaattgtgtt
     1081 gcagattata gtgtcctgta taatagcgca tcattcagca cttttaagtg ttatggggtc
     1141 agccctacga aattgaatga cttgtgtttt accaacgttt acgcagattc tttcgtcata
     1201 cgcggcgacg aagtccgaca gattgcaccg gggcaaaccg gaaagatagc agactataat
     1261 tataagttgc cggatgactt caccggctgc gttatagcat ggaactccaa taatctcgat
     1321 tcaaaagtcg gcggcaacta taattatctt ttccgcctgt ttcgcaagtc aaatctgaag
     1381 ccctttgaac gtgacatctc caccgaaatt tatcaagctg gcagcacccc atgtaatgga
     1441 gtggaagggt ttaactgtta ttttcccctt caatcatacg gatttcaacc aacaaatggg
     1501 gtgggctatc aaccatatcg ggtggtagtc ttgagctttg agctgctcca cgcaccggct
     1561 acagtttgcg gaccgaagaa aagcactaac ctggtgaaga ataaatgtgt gaacttcaac
     1621 tttaatgggc tcaccggaac aggggtgctg acagaatcaa acaagaaatt tctccctttt
     1681 caacaatttg gtagagacat tgccgatacc acagatgccg tccgcgatcc gcaaacactg
     1741 gaaatcctcg atataacacc ctgtagcttc ggcggggtca gcgtgataac tcccggaacg
     1801 aacacatcca accaagtggc tgtcctgtat caaggtgtca actgtaccga agtccctgtg
     1861 gcgatacacg ccgaccaact gacacccacc tggagagtct actcaacagg ttccaatgtc
     1921 tttcaaacta gagcgggttg tctcattggc gctgagcatg tcaataattc atacgaatgt
     1981 gatattccta ttggggcagg aatctgtgcg tcttatcaaa ctcaaacgaa ctcaccacgg
     2041 cgcgctcgct cagtagccag ccagtccgta atcgcctata ccatgagtct cggggctgag
     2101 aactccgtgg cttattcaaa caattccatc gcaatcccaa ctaactttac cattagcgtt
     2161 accacggaaa tcttgcccgt cagcatgact aagacgagcg tagactgtac gatgtatatc
     2221 tgtggggatt ccaccgagtg tagcaacctc ttgcttcaat acgggtcttt ctgtactcaa
     2281 ctcaaccgag cattgaccgg aattgcggtc gaacaagaca agaataccca agaggtgttc
     2341 gcacaagtaa agcaaatata caaaacgccg ccaatcaaag attttggcgg gtttaatttt
     2401 agccaaatac tgccagaccc atctaagcct tcaaaacgca gctttatcga ggatctgctg
     2461 ttcaacaaag ttacgttggc tgacgcgggt ttcattaagc agtatgggga ttgtttggga
     2521 gacattgcgg cccgcgacct tatctgtgct caaaaattca atgggttgac agttctgccc
     2581 cctctgctca ctgatgagat gattgcacag tatacatcag cattgttggc aggaaccatt
     2641 acatccggct ggacttttgg cgcgggtgcg gcccttcaaa tacctttcgc tatgcaaatg
     2701 gcttacagat tcaatgggat tggtgtcacg caaaatgtat tgtatgaaaa tcagaagctg
     2761 atcgctaatc aatttaacag cgcgattggc aagatccaag attcactgtc aagcacagcg
     2821 tccgccctcg gcaaactcca agatgttgtg aatcaaaacg cccaggccct gaataccctc
     2881 gtgaagcaac tgagttctaa cttcggcgca atctccagcg tcctcaacga catattgtcc
     2941 agacttgata aggtggaagc cgaggtgcaa attgatcggc tcatcacagg caggctccag
     3001 tcactccaaa cttatgtgac acaacagttg atccgggctg ctgagatacg agcaagcgcc
     3061 aacctggccg ccactaaaat gtcagagtgc gtgctcgggc agtccaaacg ggttgacttt
     3121 tgtggcaagg gctatcactt gatgtccttc ccccaatccg ccccacatgg agtggtcttt
     3181 ctccacgtca cttatgtccc tgctcaagag aagaacttta cgacagctcc agcaatctgt
     3241 cacgatggca aagcgcactt tcccagggaa ggcgttttcg tcagtaatgg cacgcattgg
     3301 tttgtgaccc aaaggaattt ctatgaaccc caaatcataa ccactgacaa tacatttgtc
     3361 tcaggcaact gtgatgtggt cattgggatc gtgaacaaca cggtatatga tcccctgcaa
     3421 cccgagttgg atagcttcaa agaggaattg gataaatact ttaagaatca tacttcaccc
     3481 gatgttgatc tcggggacat atcaggaata aatgcaagtg tcgtcaacat tcagaaagag
     3541 attgatcgcc ttaatgaggt cgccaagaac ttgaatgaat ctttgatcga cttgcaagaa
     3601 ctgggcaaat acgaacaata tattaaatgg ccctggtaca tttggttggg ctttatcgcc
     3661 ggacttatag ctatcgttat cgtcacaatt atgttgtgct gtatgactag ctgttgttcc
     3721 tgcttgaaag gatgttgcag ttgcggatct tgttgtaaat aaaggatcct ctagaagttg
     3781 tctcctcctg cactgactga ctgatacaat cgatttctgg atccgcaggc ctaatcaacc
     3841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4201 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgtctagc ttgactgact
     4441 gagatacagc gtaccttcag ctcacagaca tgataagata cattgatgag tttggacaaa
     4501 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4561 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4621 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4681 gtggtattgg cccatctcta tcggtatcgt agcataaccc cttggggcct ctaaacgggt
     4741 cttgaggggt tttttgtgcc cctcgggccg gattgctatc taccggcatt ggcgcagaaa
     4801 aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg
     4861 atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt
     4921 aaggtcgtca gctatcctgc aggcgatctc tcgatttcga tcaagacatt cctttaatgg
     4981 tcttttctgg acaccactag gggtcagaag tagttcatca aactttcttc cctccctaat
     5041 ctcattggtt accttgggct atcgaaactt aattaagcga tctgcatctc aattagtcag
     5101 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5161 attctccgcc ccatcgctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     5281 gaggtagcca acatgattga acaagatgga ttgcacgcag gttctcccgc cgcttgggtg
     5341 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5401 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5461 ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5521 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5581 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5641 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5701 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5761 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5821 cggatgcccg acggcgagga tctcgtcgtg acccacggcg atgcctgctt gccgaatatc
     5881 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5941 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6001 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6061 tatcgccttc ttgacgagtt cttctagtat gtaagccctg tgccttctag ttgccagcca
     6121 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6181 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6241 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6301 ggggatgcgg tgggctctat ggttaattaa ccagtcaagt cagctacttg gcgagatcga
     6361 cttgtctggg tttcgactac gctcagaatt gcgtcagtca agttcgatct ggtccttgct
     6421 attgcacccg ttctccgatt acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa
     6481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7321 tatttcgttc atccatagtt gcatttaaat ttccgaactc tccaaggccc tcgtcggaaa
     7381 atcttcaaac ctttcgtccg atccatcttg caggctacct ctcgaacgaa ctatcgcaag
     7441 tctcttggcc ggccttgcgc cttggctatt gcttggcagc gcctatcgcc aggtattact
     7501 ccaatcccga atatccgaga tcgggatcac ccgagagaag ttcaacctac atcctcaatc
     7561 ccgatctatc cgagatccga ggaatatcga aatcggggcg cgcctggtgt accgagaacg
     7621 atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc cagtcggaat
     7681 ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg gtcacggcag
     7741 cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta taatcgagtc
     7801 ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg catgagtatt
     7861 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7921 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc gcgagtgggt
     7981 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgc
     8041 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8101 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
     8161 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8221 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
     8281 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8341 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8401 atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8461 cagttgatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8521 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8581 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8641 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8701 aagcattggt aaccgattct aggtgcattg gcgcagaaaa aaatgcctga tgcgacgctg
     8761 cgcgtcttat actcccacat atgccagatt cagcaacgga tacggcttcc ccaacttgcc
     8821 cacttccata cgtgtcctcc ttaccagaaa tttatcctta agatcccgaa tcgtttaaac
     8881 tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc cgtggcgctc
     8941 atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt tggcagcgat
     9001 cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct ctagtggtca
     9061 taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt ttagaggccc
     9121 caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac acatccatct
     9181 tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccagat ctagtaatca
     9241 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9301 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9361 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9421 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9481 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9541 cctacttggc agtacatcta cgtattagtc atcgctatta ccatgctgat gcggttttgg
     9601 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     9661 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     9721 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     9781 agcagagctg gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     9841 ctccatagaa gacaccggga ccgatccagc ctcccctcga agcttacatg tggtaccgag
     9901 ctcggatcct gagaacttca gggtgagtct atgggaccct tgatgttttc tttccccttc
     9961 ttttctatgg ttaagttcat gtcataggaa ggggagaagt aacagggtac acatattgac
    10021 caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt
    10081 ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata
    10141 caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag
    10201 gcaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag
    10261 gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg
    10321 ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct
    10381 cttatcttcc tcccacagct cctgggcaac gtgctggtct gtgtgctggc ccatcacttt
    10441 ggcaaaggcg gccgcc
//
