LOCUS       sequence-431628-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike D80A,D215G,L242-L244 
            deletion,E484K,N501Y,D614G,A701V for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 SA190 VOC-501...
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3830..4418
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3883..3903)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4301..4312)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4471..4592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4482..4501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4536..4555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4707..4753
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4783..4919
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4964..5035
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5080..5276
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5127..5262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5290..6084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5344..5363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5954..5973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6095..6319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6515..7103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6595..6614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7849..7917,7918..8709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8067..8086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8722..8858
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9010..9081
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9086..9132)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9150..9198
                     /note="synthetic polyadenylation signal"
     enhancer        9279..9582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9584..9786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9737..9757
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9920..10395
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atgtttgtgt tcctcgttct cctgcccctc gtttcttccc aatgcgtgaa cctcacaact
       61 agaacgcagc tcccacccgc ttacacgaac tcttttactc gtggagtata ctatcccgat
      121 aaagtcttta ggtccagtgt ccttcattct actcaagacc ttttcctgcc attcttctca
      181 aatgtcacct ggttccatgc tatccacgtt tctggcacca acggaactaa acgatttgct
      241 aatcccgtct tgccatttaa cgatggcgta tactttgcct caacggagaa gtccaacatt
      301 atccggggat ggatatttgg caccaccctg gactccaaga cacagtctct cctcattgtg
      361 aacaacgcca ccaatgtcgt catcaaagtc tgtgaatttc aattttgtaa cgatcccttt
      421 ctcggggttt attatcataa gaataacaag tcttggatgg agtcagagtt ccgcgtttac
      481 agttccgcta acaactgtac ttttgagtac gtttcccaac catttctcat ggatcttgaa
      541 gggaagcaag gcaacttcaa gaatctccgc gaatttgtgt ttaagaatat agacggctac
      601 ttcaaaattt atagcaagca taccccgata aatctggttc ggggtctccc tcaaggcttc
      661 tccgcgttgg aacccctcgt ggacttgccg attggcatca acatcacccg gttccaaacc
      721 cttcatcgaa gctacctcac gcctggcgac agtagtagtg gttggacagc gggcgcagca
      781 gcctactatg tgggatattt gcaaccacgc acctttcttc tcaaatataa cgaaaacgga
      841 acaattaccg atgccgtaga ctgtgccctc gatcccctgt ccgaaactaa atgcacactc
      901 aagtccttca cagttgagaa aggcatatac caaaccagta acttccgcgt ccaacccacc
      961 gaatccattg ttcgattccc taacatcacc aatttgtgtc ctttcggtga agtatttaat
     1021 gccactcgat ttgcatcagt ttatgcctgg aatcgcaagc gaatatcaaa ttgtgttgca
     1081 gattatagtg tcctgtataa tagcgcatca ttcagcactt ttaagtgtta tggggtcagc
     1141 cctacgaaat tgaatgactt gtgttttacc aacgtttacg cagattcttt cgtcatacgc
     1201 ggcgacgaag tccgacagat tgcaccgggg caaaccggaa agatagcaga ctataattat
     1261 aagttgccgg atgacttcac cggctgcgtt atagcatgga actccaataa tctcgattca
     1321 aaagtcggcg gcaactataa ttatctttac cgcctgtttc gcaagtcaaa tctgaagccc
     1381 tttgaacgtg acatctccac cgaaatttat caagctggca gcaccccatg taatggagtg
     1441 aaagggttta actgttattt tccccttcaa tcatacggat ttcaaccaac atatggggtg
     1501 ggctatcaac catatcgggt ggtagtcttg agctttgagc tgctccacgc accggctaca
     1561 gtttgcggac cgaagaaaag cactaacctg gtgaagaata aatgtgtgaa cttcaacttt
     1621 aatgggctca ccggaacagg ggtgctgaca gaatcaaaca agaaatttct cccttttcaa
     1681 caatttggta gagacattgc cgataccaca gatgccgtcc gcgatccgca aacactggaa
     1741 atcctcgata taacaccctg tagcttcggc ggggtcagcg tgataactcc cggaacgaac
     1801 acatccaacc aagtggctgt cctgtatcaa ggtgtcaact gtaccgaagt ccctgtggcg
     1861 atacacgccg accaactgac acccacctgg agagtctact caacaggttc caatgtcttt
     1921 caaactagag cgggttgtct cattggcgct gagcatgtca ataattcata cgaatgtgat
     1981 attcctattg gggcaggaat ctgtgcgtct tatcaaactc aaacgaactc accacggcgc
     2041 gctcgctcag tagccagcca gtccataatc gcctatacca tgagtctcgg ggttgagaac
     2101 tccgtggctt attcaaacaa ttccatcgca atcccaacta actttaccat tagcgttacc
     2161 acggaaatct tgcccgtcag catgactaag acgagcgtag actgtacgat gtatatctgt
     2221 ggggattcca ccgagtgtag caacctcttg cttcaatacg ggtctttctg tactcaactc
     2281 aaccgagcat tgaccggaat tgcggtcgaa caagacaaga atacccaaga ggtgttcgca
     2341 caagtaaagc aaatatacaa aacgccgcca atcaaagatt ttggcgggtt taattttagc
     2401 caaatactgc cagacccatc taagccttca aaacgcagct ttatcgagga tctgctgttc
     2461 aacaaagtta cgttggctga cgcgggtttc attaagcagt atggggattg tttgggagac
     2521 attgcggccc gcgaccttat ctgtgctcaa aaattcaatg ggttgacagt tctgccccct
     2581 ctgctcactg atgagatgat tgcacagtat acatcagcat tgttggcagg aaccattaca
     2641 tccggctgga cttttggcgc gggtgcggcc cttcaaatac ctttcgctat gcaaatggct
     2701 tacagattca atgggattgg tgtcacgcaa aatgtattgt atgaaaatca gaagctgatc
     2761 gctaatcaat ttaacagcgc gattggcaag atccaagatt cactgtcaag cacagcgtcc
     2821 gccctcggca aactccaaga tgttgtgaat caaaacgccc aggccctgaa taccctcgtg
     2881 aagcaactga gttctaactt cggcgcaatc tccagcgtcc tcaacgacat attgtccaga
     2941 cttgataagg tggaagccga ggtgcaaatt gatcggctca tcacaggcag gctccagtca
     3001 ctccaaactt atgtgacaca acagttgatc cgggctgctg agatacgagc aagcgccaac
     3061 ctggccgcca ctaaaatgtc agagtgcgtg ctcgggcagt ccaaacgggt tgacttttgt
     3121 ggcaagggct atcacttgat gtccttcccc caatccgccc cacatggagt ggtctttctc
     3181 cacgtcactt atgtccctgc tcaagagaag aactttacga cagctccagc aatctgtcac
     3241 gatggcaaag cgcactttcc cagggaaggc gttttcgtca gtaatggcac gcattggttt
     3301 gtgacccaaa ggaatttcta tgaaccccaa atcataacca ctgacaatac atttgtctca
     3361 ggcaactgtg atgtggtcat tgggatcgtg aacaacacgg tatatgatcc cctgcaaccc
     3421 gagttggata gcttcaaaga ggaattggat aaatacttta agaatcatac ttcacccgat
     3481 gttgatctcg gggacatatc aggaataaat gcaagtgtcg tcaacattca gaaagagatt
     3541 gatcgcctta atgaggtcgc caagaacttg aatgaatctt tgatcgactt gcaagaactg
     3601 ggcaaatacg aacaatatat taaatggccc tggtacattt ggttgggctt tatcgccgga
     3661 cttatagcta tcgttatggt cacaattatg ttgtgctgta tgactagctg ttgttcctgc
     3721 ttgaaaggat gttgcagttg cggatcttgt tgtaaataaa ggatcctcta gaagttgtct
     3781 cctcctgcac tgactgactg atacaatcga tttctggatc cgcaggccta atcaacctct
     3841 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3901 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3961 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4021 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4081 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4141 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4201 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4261 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4321 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4381 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtctagcttg actgactgag
     4441 atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca
     4501 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4561 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4621 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4681 gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt
     4741 gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa
     4801 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     4861 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     4921 gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct
     4981 tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc
     5041 attggttacc ttgggctatc gaaacttaat taagcgatct gcatctcaat tagtcagcaa
     5101 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5161 ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5221 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaggag
     5281 gtagccaaca tgattgaaca agatggattg cacgcaggtt ctcccgccgc ttgggtggag
     5341 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5401 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5461 aatgaactcc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5521 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5581 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5641 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5701 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5761 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg
     5821 atgcccgacg gcgaggatct cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg
     5881 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5941 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6001 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6061 cgccttcttg acgagttctt ctagtatgta agccctgtgc cttctagttg ccagccatct
     6121 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6181 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6241 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6301 gatgcggtgg gctctatggt taattaacca gtcaagtcag ctacttggcg agatcgactt
     6361 gtctgggttt cgactacgct cagaattgcg tcagtcaagt tcgatctggt ccttgctatt
     6421 gcacccgttc tccgattacg agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg
     6481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7321 ttcgttcatc catagttgca tttaaatttc cgaactctcc aaggccctcg tcggaaaatc
     7381 ttcaaacctt tcgtccgatc catcttgcag gctacctctc gaacgaacta tcgcaagtct
     7441 cttggccggc cttgcgcctt ggctattgct tggcagcgcc tatcgccagg tattactcca
     7501 atcccgaata tccgagatcg ggatcacccg agagaagttc aacctacatc ctcaatcccg
     7561 atctatccga gatccgagga atatcgaaat cggggcgcgc ctggtgtacc gagaacgatc
     7621 ctctcagtgc gagtctcgac gatccatatc gttgcttggc agtcagccag tcggaatcca
     7681 gcttgggacc caggaagtcc aatcgtcaga tattgtactc aagcctggtc acggcagcgt
     7741 accgatctgt ttaaacctag atattgatag tctgatcggt caacgtataa tcgagtccta
     7801 gcttttgcaa acatctatca agagacagga tcagcaggag gctttcgcat gagtattcaa
     7861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcgcg agtgggttac
     7981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgcttt
     8041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca
     8161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag
     8281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8401 gcaacaacct tgcgtaaact attaactggc gaactactta ctctagcttc ccggcaacag
     8461 ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8701 cattggtaac cgattctagg tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc
     8761 gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac
     8821 ttccatacgt gtcctcctta ccagaaattt atccttaaga tcccgaatcg tttaaactcg
     8881 actctggctc tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt
     8941 tgctcgtcgg gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc
     9001 ggctcccgac atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa
     9061 cagcagcttc agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa
     9121 ggggttatgc tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg
     9181 atggatagcg attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt
     9241 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     9301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     9361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     9421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     9481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     9541 acttggcagt acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag
     9601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     9781 agagctggtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     9841 catagaagac accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc
     9901 ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt
     9961 tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa
    10021 atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt
    10081 tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa
    10141 tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca
    10201 atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt
    10261 tcatattgct aatagcagct acaatccagc taccattctg cttttatttt atggttggga
    10321 taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt
    10381 atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc
    10441 aaaggcggcc gcc
//
