LOCUS       sequence-431654-        8824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for the production of lentiviral vectors that can be
            used to identify and live sort pancreatic progenitor expresssing the
            Keratin7 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  TITLE     Lentiviral plasmid for expression of GFP under the control of the 
            Keratin7 (KRT7) promoter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCLX-KRT7-GFP
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2520..2540
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4047..4071
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4075..4084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4081..4083,4084..4086,4087..4800)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4081..4083,4084..4086,4087..4797)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4126..4147)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4387..4406)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4734..4755
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4801..4825)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4843..5431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4896..4916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5314..5325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5574..5607)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5627..5807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5845..5864)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5910..5965
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5964..5983)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6326..6342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6326..6342)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6339..6361)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6350..6366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6374..6380,6381..6398,6399..6404))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6419..6440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6499..6694
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6545..6680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6700..6834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6737..6756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6791..6810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6902..6919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7073..7661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7153..7172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7832..8623,8624..8692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8455..8474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8693..8797)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat actggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatctagtga ttatcggatc
     2521 aactttgtat agaaaagttg gctagcactg gagttttaaa gaatgttcca gggaggggta
     2581 agaggcccag ggcagatgtg gggtaggctc aggaatgagg aagtatttct caagcctgtg
     2641 tgtggtggtt atagggggcc gaggcctgga ctgaagtgcc caggtacttt tgtctgtgtg
     2701 cttcccagct cttgttagac cctcatcctg aaggagctgt ggctgagggg gccaggaccc
     2761 gtccaggtca gcgcactagc tgtgccctgc tggggctcag cttgagaaag agaaggagct
     2821 cttggtgtgg gtgagaaaca acctccaaag tgggggatgg gtggcagggg ctgtagcagg
     2881 atggaaagtg gggtgcagag ggggacaaag gactgaggaa gcaccttgag gggtagggag
     2941 tctccttgag agcttagggt tgtctacctg aaaaggcaaa gcaggaagaa tatgacattc
     3001 ccatcctccc cacctaggac gcccctaaag gaccagtctc actgcatgga gccagaggtg
     3061 ggctaaaggc tgtccaagtg agtctgggaa aactggaagt ccaggagcac tccgaggagc
     3121 actgagcaca ccaccacggg ttaccacaga ggccagtttt agttgattcc cttgtcaggc
     3181 atagcctagt gggtttgtaa gcaggaaaaa tgaggcagag cagggaggaa cgtctggatg
     3241 tgaacgggag acaggacaca cacatgtgca tacaaatgtg ttcagttgtg aatccagacc
     3301 ccccatgcac agactgcact catatacact cacactagtg tgttaggggt atgagggcac
     3361 agaagggtac gcacagccat cccaggaacc catacagtag tttgaggagg gggtgaggca
     3421 agccctctga acttggatgg tggggagggc gcagttactg aaacagctct tcccagtaag
     3481 gatcgtcaca gggccaggtt actcaagaac tgagctgagc tctccagtag cagctgggcc
     3541 aggaaggaga gtcctaggaa gcctagggct tccccagcca gtgggtgagg ggtttcaact
     3601 ttcagcagga gctaaagttt gtcaggcact tccttttgta gcacttggct ttcctactgg
     3661 cctcaggaat ctaggtattc actcctcata cctgctctag gaagccttct cctgccaatt
     3721 gtaaggtggt ctctttgcgg tccagagatt tctgggctgt gggtatcttg gacaacttgc
     3781 ctgaggtgtg ggtgtctcac gacggcgtct cggcctcctg cagacacttc ccgagtggac
     3841 gaagagcctt gggcggcggc tgggggcggt gcgagggcag aaggcaggcg gacaggaagg
     3901 ccctggctgc agagaaaggt ggccagcggc cagccccgcc ctgtccccac ctgtgtgcgc
     3961 tcagcctccg ccccgcatgg aggaataaaa ggtcgcagac tgcctgaggt cagggagttc
     4021 gcgctgctcc tcgcctcgtc cagccagcaa gtttgtacaa aaaagcaggc tcccgccacc
     4081 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4141 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4201 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4261 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4321 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4381 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4441 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4501 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4561 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4621 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4681 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4741 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4801 gacccagttt cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac
     4861 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4921 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4981 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5041 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5101 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5161 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5221 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5281 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5341 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5401 agtcggatct ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgctttaaga
     5461 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5521 gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt
     5581 cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt
     5641 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5701 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5761 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagttgaa
     5821 tcgccagtgt gtcgagctag cagatctttt tccctctgcc aaaaattatg gggacatcat
     5881 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     5941 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     6001 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     6061 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     6121 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     6181 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     6241 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     6301 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6361 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6421 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6481 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6541 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6601 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6661 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     6721 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6781 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     6841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8821 ctgg
//
