LOCUS       sequence-431658-       11784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for the production of lentiviral vectors that can be
            used to track pluripotent stem cells, such as ES cells, iPS and 
            tissue-resident pluripotent cells. GFP expression is lost upon diff.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11784)
  TITLE     Lentiviral plasmid for expression of GFP under the control of the 
            Oct4 promoter and enhancer. Live visualization and sorting of ES 
            cells and totipotent cells in tissues.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RIX-F2Oct4F3-GFP
FEATURES             Location/Qualifiers
     source          1..11784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5135..5252
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     5272..5288
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5273..5289
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6620..6629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6626..6628,6629..6631,6632..7345)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6626..6628,6629..6631,6632..7342)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6671..6692)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6932..6951)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7279..7300
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7361..7949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7414..7434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7832..7843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8330..8363)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8383..8563
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8615..8736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8652..8671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8706..8725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8776..8911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8931..8950)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8932..8950)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8960..8977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8960..8976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8969..8991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9118..9573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9205..9224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9415..9436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9599..9703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9704..9772,9773..10564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9922..9941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10735..11323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11224..11243
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11477..11494
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11611..11632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11647..11652,11653..11670,11671..11677)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11685..11701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11690..11712
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11709..11725
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11709..11725
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11744..11764
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11746..11764
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttgatcc cattactggc ctggtgctta gttatctttg caagagaata gccctcagag
     1861 tgcctgtctg caagggatgg tgtttgtctg tctgctccta caccatgctc tgaacgaagg
     1921 ccccagggtg aatcacacag cctcaaaaag cccagagcac cccacagtgt acatagccag
     1981 gacagagttc tgagcctggg cccagcccgc ctgagctgtt ggctagggtc agtggctcta
     2041 ggtgccctgg ggggcggggg tgtctcttga tggtagtctt catcaggtag agatggaacc
     2101 tttctctata aaacaagctg cccttgaact cctgataatt ctcctgcctc agcttcatcg
     2161 acttcaccaa attcaccctg agactttgaa aaaaaaattg cgttttattt taagggtgtg
     2221 tatgtaccta cacacagcta cacatagcac atgtgtggaa gtcccctcct tctaccacat
     2281 gggtccaagt catcccccag ggtggctgag gggccatctc tctggccctc tccatgaatc
     2341 ttccgtttcc tccactctgt catgctcacc tcccaatttc tatacattca ttgtggaaca
     2401 gtgccatagg ttagttggaa gacctaaata aaggcagcga cttggaagcc gccaagttca
     2461 caaagcttcc tcaatagcag attaaggaag ggctaggacg agagggaccc ctccccaacc
     2521 atcttctctg cccccagggc agagctatca tgcacctttg ttatgcatct gccgtctgcc
     2581 cagaactctc aacctccctg gggggagggg cagggtcggg ctacaacctc cccacaccca
     2641 gttcctccca gggccagcct ctaaggccta gacagcactc tccaccacag ttcaagtatg
     2701 cctgcagccc agcagtcctg tctgtattca ataccaacct tgtcttatgg attgtgattt
     2761 ctcttttggt gactcactgg ccaggacaag agacatattc tgagtccttt aactgcctag
     2821 tgactggctt tgcaaccagg ttagccctaa gcgtgcctag agtataatac agtccttaac
     2881 agcaactttg tctgaagtcc caagtcttct tagaactagc ttgaaacaga tgtgccctag
     2941 cctggtcccg agctgtgagc ctggtggccc tggagatggg acagcagacc tgttggctca
     3001 tctgatccag tttcttgcct cctgggtctt agaaaatcat cagactaact tttgggtttg
     3061 attcaagggt tccctacata gccttagatg gcttggaact cactctgtag accaggctgg
     3121 ccttgaactc acagagatcc accagccttt gcttcctgag tgctaggatt gaaaatatgt
     3181 gccactctgc cccctttaag agtcactttg ggccgggtgc ggtcccaact catggcttta
     3241 atcccagcac ttgggaggca gaggcaggtg gatttctgag ttccaggcca gcctggtcta
     3301 cagagtgagt tccaggacag ccagggcttc acagagaaat cctgtctcga aaaaaaaaaa
     3361 aaatctgtca ccctcttctg gtgtctgaag tacttacgta caataataaa taaatctttt
     3421 taaaaaaaaa aaagaaagaa agaaaaacaa acaaaaaaga gtcaccatgg tatcttaaat
     3481 accccaaatt tgagctaaaa ttcgttaact tgccacaaac cacctgtatt ttagaaccaa
     3541 gtcaagatct gtctcggagt tctgtctgga gaaccaaaga cagcagagaa gcccacacgt
     3601 ccccagccag agatggtcca ccagcatgaa aaggtatcct gggaagccag atgcggaatg
     3661 ccacacctat aatgcctgcc cttgggcgtt ggaagtgata agaacaaagg tccaaggcta
     3721 ggggctggga aagaactcag catttgagtg tacattgctc tttcagagga cccttttaaa
     3781 attctgttcc tagcacccat attgggtggt ttacagctag gagaacagaa atctcttggc
     3841 tgctgtggtc atctgcagtt acatggacac acatatatac acatgatttt cttttaaaga
     3901 gaatttaagg ccagccttaa acacaagagt cctccaggcc agcttgagcc agcctcagca
     3961 ggagaccttg tctaaaacaa aacaaacaaa caataggcgt tgacccccac agctttctta
     4021 ctgtgagttc agccacctcc tcacagactg ggtgatcagt taaatggact cacggaggac
     4081 actagactag agcagggaaa tcactcgtgt tagcatccag gctgggtctc cctatgaagc
     4141 catagggaga aatgaggagt ttcagcgaca tgagctcagt caggcctgcc ccagccagga
     4201 acagcttcct tgctggttag catccccctc ccccaacccc aacccccagc cgccctggga
     4261 ccagcaacgt ctcttcccaa atccagatgt tctggaaggg aggaggtagt ttctctggct
     4321 ggcatagatt ccttagctaa ctgcatctcc ctttccttgt cttggttttc ttgcctaatc
     4381 aaaaagactt gcagggggca ggaggggggg ggggggttgg agggggagac ttgaggggag
     4441 gcctgagagg aacctgagga cagctcagca agtaaccaca cttgctgagc ctaacgacca
     4501 tggatttgat ccctggggtc catttcaaag aagcatttga tctccagagg ttcgatattg
     4561 atattgcact cagatacaca cacacacaca atacaataaa aattaaaaga aaaaacaaag
     4621 atctagaagc tgaaggtagg aataggtcac cagcagaaca gaaggcttgc ggaatgtgcc
     4681 tgctcccttt gaaataaatg agttctattt ctcccttttt ttttttttct ttctgagaca
     4741 gggtttctct gtgtagccct ggctgtcctg gaactcactc tgtagaccag gctggccttg
     4801 agctcagaaa tcagcctgcc ctctgcttcc caagtgctgg gattataggc attcgccact
     4861 actgcccgtt tccttttttc ttctgagaca aatttacatg tagcagaggc tgcccctaaa
     4921 ctctacgtag ccaagaaggc ccttgaactt ctgatcctct tgccttccca gttctgatga
     4981 gcagtgtcca aatgcctttt agacttgcag aaatggcttc accactacac ccaaggctgc
     5041 ccctgataac cagaacccca cgacatcact cattcaagat aaccagccac cttgatctgc
     5101 cttgtaggtg gagagtctcc ccgctaggat caacttttaa aagaaaaggg gggattgggg
     5161 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     5221 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggtcg
     5281 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatcctca gactgggccc
     5341 agaaaaccac tctagggaag ttcagggtag gctctctgca ccccctcctc ctaatcccgt
     5401 ctccttagtg tctttccgcc agcacaggaa tgggggaggg gtgggtgacg aggatgaaca
     5461 ccggagtccc tggaggaagg gaagcagggt atctccatct gaggctctgt ctttgaggag
     5521 aggtggagag ctggggaagt cttgtgtgag gggattgggg ctcaggaggg ggttggggag
     5581 caggaagttg tccccagggg agccatcctg gcccattcaa gggttgagta cttgtttagg
     5641 gttagagctg ccccctctgg ggaccaggat tgtccagcca aggccattgt cctgccccct
     5701 tcccccagtc cctcccaggc ccctttgaac ctgaagtcag atatttcttc tctctaccca
     5761 cctcccaccc gttgggtttc tccacccagg aactaggctg gaagcctggg atgaggaggt
     5821 ggggggaggg agaactgaga atcttgagga aagaggcccc ggccttaact gtgaggggat
     5881 ggagcctggg tgcaggtctt atgggggttg gggggtggtt agtgtctaat ctaccaacct
     5941 ggacaacaca agatggaata ctgtgctctg aaaacgcaga gccagcactt ctctggggtc
     6001 tctggggaca tatctggttg gggctcgggg tcccatggtg tagagcctct aaactctgga
     6061 ggactggagg tgcaatggct gtcttgtcct ggccttggac atgggctgaa atactgggtt
     6121 cacccatatc taggactcta gacgggtggg taagcaagaa ctgaggagtg gccccagaaa
     6181 taattggcac acgaacattc aatggatgtt ttaggctctc cagaggatgg ctgagtgggc
     6241 tgtaaggaca ggccgagagg gtgcagtgcc aacaggcttt gtggtgcgat ggggcatccg
     6301 agcaactggt ttgtgaggtg tccggtgacc caaggcaggg gtgagaggac cttgaaggtt
     6361 gaaaatgaag gcctcctggg gtcccgtcct aagggttgtc ctgtccagac gtccccaacc
     6421 tccgtctgga agacacaggc agatagcgct cgcctcagtt tctcccaccc ccacagctct
     6481 gctcctccac ccacccaggg ggcggggcca gaggtcaagg ctagagggtg ggattgggga
     6541 gggagaggtg aaaccgtccc taggtgagcc gtctttccac caggcccccg gctcggggtg
     6601 cccaccttcc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     6661 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     6721 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     6781 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     6841 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6901 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     6961 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7021 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     7081 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     7141 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7201 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     7261 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     7321 cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc tggattacaa
     7381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7741 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7921 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
     7981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8041 gggctaattc actcccaacg aagacaagat gtgatcctcc tagagggaca gccccccccc
     8101 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     8161 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     8221 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     8281 gagcctgcag acacctgggg ggatacgggg aaaaagcttt tctagtggaa taacttcgta
     8341 tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac
     8401 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8461 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8521 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gagcggccaa
     8581 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8701 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8761 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8821 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8881 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     8941 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     9001 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     9061 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     9121 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9181 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9241 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9301 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9361 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9421 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9481 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9541 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     9601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10381 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10861 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    11161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    11521 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11581 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11641 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    11701 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    11761 aagggaacaa aagctggagc tgca
//
