LOCUS       sequence-431667-        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LGP2-Myc expression vector for overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Takahashi T, Nakano Y, Onomoto K, Yoneyama M, Ui-Tei K
  TITLE     Virus Sensor RIG-I Represses RNA Interference by Interacting with 
            TRBP through LGP2 in Mammalian Cells.
  JOURNAL   Genes (Basel). 2018 Oct 19;9(10):511. doi: 10.3390/genes9100511.
  PUBMED    30347765
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2018-10-19"; volume: "9(10):511. doi"; pages: " 
            10.3390/genes9100511"
COMMENT     Sequence Label: pEF-BOS-LGP2-Myc
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2254..2464
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2276..2472
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2323..2458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2559..3737
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2790..3728
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3685..3705
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5808..5819
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5829..5852
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5853..5870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6594..6611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6594..6610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6603..6625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6823..7278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6910..6929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7120..7141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7295..7314)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7414..7436
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt aattccctcc ccagcaggca gaagtatgca aagcatgcat
     2281 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2401 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2461 ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca
     2521 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2581 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2641 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2701 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2761 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2821 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2881 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2941 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3001 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3061 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3121 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3181 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3241 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg tcgtgtatcg
     3301 ccccgccctg ggcggcaagg ctggtctggt cggcaccagt tgcgtgagcg gaaagatggc
     3361 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3421 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3481 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgtgc ttttggagta
     3541 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3601 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3661 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3721 catttcaggt gtcgtgagga attctctaga gatccctcga cctcgagatg gagcttcggt
     3781 cctaccaatg ggaggtgatc atgcctgccc tggagggcaa gaatatcatc atctggctgc
     3841 ccacgggtgc cgggaagacc cgggcggctg cttatgtggc caagcggcac ctagagactg
     3901 tggatggagc caaggtggtt gtattggtca acagggtgca cctggtgacc cagcatggtg
     3961 aagagttcag gcgcatgctg gatggacgct ggaccgtgac aaccctgagt ggggacatgg
     4021 gaccacgtgc tggctttggc cacctggccc ggtgccatga cctgctcatc tgcacagcag
     4081 agcttctgca gatggcactg accagccccg aggaggagga gcacgtggag ctcactgtct
     4141 tctccctgat cgtggtggat gagtgccacc acacgcacaa ggacaccgtc tacaacgtca
     4201 tcatgagcca gtacctagaa cttaaactcc agagggcaca gccgctaccc caggtgctgg
     4261 gtctcacagc ctccccaggc actggcgggg cctccaaact cgatggggcc atcaaccacg
     4321 tcctgcagct ctgtgccaac ttggacacgt ggtgcatcat gtcaccccag aactgctgcc
     4381 cccagctgca ggagcacagc caacagcctt gcaaacagta caacctctgc cacaggcgca
     4441 gccaggatcc gtttggggac ttgctgaaga agctcatgga ccaaatccat gaccacctgg
     4501 agatgcctga gttgagccgg aaatttggga cgcaaatgta tgagcagcag gtggtgaagc
     4561 tgagtgaggc tgcggctttg gctgggcttc aggagcaacg ggtgtatgcg cttcacctga
     4621 ggcgctacaa tgacgcgctg ctcatccatg acaccgtccg cgccgtggat gccttggctg
     4681 cgctgcagga tttctatcac agggagcacg tcactaaaac ccagatcctg tgtgccgagc
     4741 gccggctgct ggccctgttc gatgaccgca agaatgagct ggcccacttg gcaactcatg
     4801 gcccagagaa tccaaaactg gagatgctgg aaaagatcct gcaaaggcag ttcagtagct
     4861 ctaacagccc tcggggtatc atcttcaccc gcacccgcca aagcgcacac tccctcctgc
     4921 tctggctcca gcagcagcag ggcctgcaga ctgtggacat ccgggcccag ctactgattg
     4981 gggctgggaa cagcagccag agcacccaca tgacccagag ggaccagcaa gaagtgatcc
     5041 agaagttcca agatggaacc ctgaaccttc tggtggccac gagtgtggcg gaggaggggc
     5101 tggacatccc acattgcaat gtggtggtgc gttatgggct cttgaccaat gaaatctcca
     5161 tggtccaggc caggggccgt gcccgggccg atcagagtgt atacgcgttt gtagcaactg
     5221 aaggtagccg ggagctgaag cgggagctga tcaacgaggc gctggagacg ctgatggagc
     5281 aggcagtggc tgctgtgcag aaaatggacc aggccgagta ccaggccaag atccgggatc
     5341 tgcagcaggc agccttgacc aagcgggcgg cccaggcagc ccagcgggag aaccagcggc
     5401 agcagttccc agtggagcac gtgcagctac tctgcatcaa ctgcatggtg gctgtgggcc
     5461 atggcagcga cctgcggaag gtggagggca cccaccatgt caatgtgaac cccaacttct
     5521 cgaactacta taatgtctcc agggatcctg tggtcatcaa caaagtcttc aaggactgga
     5581 agcctggggg tgtcatcagc tgcaggaact gtggggaggt ctggggtctg cagatgatct
     5641 acaagtcagt gaagctgcca gtgctcaaag tccgcagcat gctgctggag acccctcagg
     5701 ggcggatcca ggccaaaaag tggtcccgcg tgcccttctc cgtgcctgac tttgacttcc
     5761 tgcagcattg tgccgagaac ttgtcggacc tctccctgga cagatccatt gaaggaagga
     5821 tcgtcaagga ctacaaggac gacgatgaca agcaccacca ccaccaccac tgagcggccg
     5881 cgactctaga gtgagggtcc ccacctggga cccttgagag tatcaggtct cccacgtggg
     5941 agacaagaaa tccctgttta atatttaaac agcagtgttc cccatctggg tccttgcacc
     6001 cctcactctg gcctcagccg actgcacagc ggcccctgca tccccttggc tgtgaggccc
     6061 ctggacaagc agaggtggcc agagctggga ggcatggccc tggggtccca cgaatttgct
     6121 ggggaatctc gtttttcttc ttaagacttt tgggacatgg tttgactccc gaacatcacc
     6181 gacgtgtctc ctgtttttct gggtggcctc gggacacctg ccctgccccc acgagggtca
     6241 ggactgtgac tctttttagg gccaggcagg tgcctggaca tttgccttgc tggacgggga
     6301 ctggggatgt gggagggagc agacaggagg aatcatgtca ggcctgtgtg tgaaaggaag
     6361 ctccactgtc accctccacc tcttcacccc ccactcacca gtgtcccctc cactgtcaca
     6421 ttgtaactga acttcaggat aataaagtgt ttgcctccaa aaaaaaaaaa aaaaaaaaaa
     6481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccgaa ttcactggcc
     6601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6721 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     6781 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     6841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     6901 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6961 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7021 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7081 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7141 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     7201 cggtctattg gttaaaaaat gagcttattt aacaaaaatt taacgcgaat tttaacaaaa
     7261 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7321 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7381 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7441 caccgtcatc accgaaacgc gcga
//
