LOCUS       sequence-431676-       10012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human P53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI TP53 S46D
FEATURES             Location/Qualifiers
     source          1..10012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        176..374
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             392..572
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    619..744
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1237..1470
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1655..1699
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1951..2067
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2090..2393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2394..2597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2594..2618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2705..3886
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     DPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     intron          3973..4202
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4185..4204
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4379..4967
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4432..4452)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4850..4861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5374..5554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5580..5597)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5586..5810
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5856..6284
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5943..5962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6153..6174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6293..6313)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6298..6627
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6478..6613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6540..6559
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6675..6722
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6741..7115
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7245..7366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7282..7301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7336..7355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7415..7431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7415..7431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7428..7450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7439..7455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7463..7469,7470..7487,7488..7493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7508..7529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7646..7663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7817..8405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7897..7916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8576..9367,9368..9436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9199..9218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9437..9541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9616..9635)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9807..10012,1..174)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
       61 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      121 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      181 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      241 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      301 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      361 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      421 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      481 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      541 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      601 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      661 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      721 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      781 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      841 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
      901 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
      961 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1021 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1081 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1141 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1201 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1261 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1321 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1381 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1441 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1501 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1561 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1621 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1681 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1741 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1801 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1861 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1921 cagatccatt cgattagtga acggatcaac ttttaaaaga aaagggggga ttggggggta
     1981 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2041 aaaacaaatt acaaaattca aaattttatc gataagcttg ggagttccgc gttacataac
     2101 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2161 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2221 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2281 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2341 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2401 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2461 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2521 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2581 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     2641 gttttgacct ccatagaaga caccgactga gctccttaag gttaactcta gagaattcgg
     2701 atccatggag gagccgcagt cagatcctag cgtcgagccc cctctgagtc aggaaacatt
     2761 ttcagaccta tggaaactac ttcctgaaaa caacgttctg tcccccttgc cgtcccaagc
     2821 aatggatgat ttgatgctgg acccggacga tattgaacaa tggttcactg aagacccagg
     2881 tccagatgaa gctcccagaa tgccagaggc tgctcccccc gtggcccctg caccagcagc
     2941 tcctacaccg gcggcccctg caccagcccc ctcctggccc ctgtcatctt ctgtcccttc
     3001 ccagaaaacc taccagggca gctacggttt ccgtctgggc ttcttgcatt ctgggacagc
     3061 caagtctgtg acttgcacgt actcccctgc cctcaacaag atgttttgcc aactggccaa
     3121 gacctgccct gtgcagctgt gggttgattc cacacccccg cccggcaccc gcgtccgcgc
     3181 catggccatc tacaagcagt cacagcacat gacggaggtt gtgaggcgct gcccccacca
     3241 tgagcgctgc tcagatagcg atggtctggc ccctcctcag catcttatcc gagtggaagg
     3301 aaatttgcgt gtggagtatt tggatgacag aaacactttt cgacatagtg tggtggtgcc
     3361 ctatgagccg cctgaggttg gctctgactg taccaccatc cactacaact acatgtgtaa
     3421 cagttcctgc atgggcggca tgaaccggag gcccatcctc accatcatca cactggaaga
     3481 ctccagtggt aatctactgg gacggaacag ctttgaggtg cgtgtttgtg cctgtcctgg
     3541 gagagaccgg cgcacagagg aggagaatct ccgcaagaaa ggggagcctc accacgagct
     3601 gcccccaggg agcactaagc gagcactgcc caacaacacc agctcctctc cccagccaaa
     3661 gaagaaacca ctggatggag aatatttcac ccttcagatc cgtgggcgtg agcgcttcga
     3721 gatgttccga gagctgaatg aggccttgga actcaaggat gcccaggctg ggaaggagcc
     3781 aggggggagc agggctcact ccagccacct gaagtccaaa aagggtcagt ctacctcccg
     3841 ccataaaaaa ctcatgttca agacagaagg gcctgactca gactgactcg agccatggaa
     3901 gcttgatatc taactgactg aaccggtggt accgaggaat taattcgctg tctgcgaggg
     3961 ccagctgttg gggtgagtac tccctctcaa aagcgggcat gacttctgcg ctaagattgt
     4021 cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg cctttgaggg
     4081 tggccgcgtc catctggtca gaaaagacaa tctttttgtt gtcaagcttg aggtgtggca
     4141 ggcttgagat ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac
     4201 aggtgtccac tcccaggtcc aactgcaggt cgagcatgca tctagggcgg ccaattccgc
     4261 cgatcctgga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     4321 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcagacaa
     4381 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4441 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4501 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4561 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4621 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4681 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4741 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     4801 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4861 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4921 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc
     4981 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5041 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5101 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5161 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     5221 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     5281 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     5341 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     5401 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5461 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5521 agaccctttt agtcagtgtg gaaaatctct agcagggccg ctttaaaccc gctgatcagc
     5581 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5641 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5701 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5761 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5821 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5881 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5941 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6001 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6061 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6121 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6181 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6241 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6301 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6361 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6421 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6481 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6541 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6601 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6661 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     6721 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     6781 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     6841 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     6901 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     6961 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7021 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7081 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7141 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7201 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7261 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9541 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     9601 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9661 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9721 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9781 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     9841 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     9901 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     9961 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg ag
//
